[Agrobacterium-mediated transformation]

Research is conducted in a team-based setting. Responsibilities are adjusted according to team members’ background and experience, and relevant training is provided. Tasks involve preparation of Agrobacterium and plant materials, plant infection and transformation experiments, bioinformatics analyses of genomic and transcriptomic data to identify key genes, and molecular genetics approaches to investigate gene functions. For related research projects and recent publications, please see our lab website: https://ipmb.sinica.edu.tw/chkuo/

• Strong interest in research and motivation to learn; relevant research experience is a plus but not required.
• English proficiency; lab meetings and documents are in English.
• A degree in Life Sciences or related fields.

The lab of Ka Wai Ma is inviting application working on a NSTC-
funded project titled Transcriptome-guided combinatorial gene
editing to understand plant-microbiota homeostasis. The Ma lab
uses both gnotobiotic Arabidopsis and the associated bacterial culture
collection to understand the principles governing plant-microbiota
homeostasis. Our research employs a multitude of omics techniques
including 16S amplicon sequencing, transcriptomics and proteomics
for functional analyses. The lab is currently looking for a motivated
candidate to work on a CRISPR editing project with subsequent
phenotyping.
The position is opened until filled
馬家威實驗室現誠聘研究助理/博士後，從事由國科會資助【以轉錄組
導向進行組合基因編輯以了解植物微生物相的平衡機理】項目。本實驗
室使用阿拉伯芥模型系統和相關的微生物庫進行植物微生物相重組實驗
以了解植物微生物群互動平衡機理。實驗室使用擴增子測序、轉錄組學
及肽組學分析等技術，並提供相關技術培訓。

Applicants are required to

1. hold a MS degree (RA) or a PhD (postdoc)
2. experience handling plants
3. have good command of English and preferably basic
knowledge of mandarin.
4. basic experience in microbiology or molecular biology
5. experience using golden gate cloning system is highly preferred
申請者要求
1. 具碩士學位 (研究助理) 或博士學位 (博士後)
2. 具植物相關操作經驗
3. 具英語交流書寫能力，通華語為佳 (非必要)
4. 具基本微生物學或份子生物學
5. 曾使用golden gate 及 Gibson 系統者為佳

Our research team is seeking 1~2 Research Associates to join us in exploring the evolution, ecology, and biology of eukaryotic microbes with key ecological roles on Earth. We focus on the interactions between microbes and their environment and utilize cutting-edge omics and bioinformatics tools to uncover their evolutionary trajectories and regulatory mechanisms. Currently, we are conducting an NSTC-funded project on marine algae, population variation, and warming. This position is open until filled.

顧銓研究室現正招募 1 至 2 位研究助理，加入我們探索真核微生物之演化、生態與生物學上的關鍵生態角色。我們著重微生物與環境之間的互動，並利用尖端的多體學與生物資訊工具發掘演化軌跡與調控機制。目前我們正執行國科會(NSTC)資助的計畫，研究海藻族群變異與暖化相關議題。本職缺即日起開放申請，額滿為止。

Research Topics
1. Population genomics and evolutionary biology of marine algae.
2. Effects of oceanographic and physico-chemical factors on algal distribution.
3. Genomic variation, transcriptomic regulation and physiological properties of lipid synthesis and host-virus interactions in marine algae.

研究主題
1. 海洋微藻族群基因體學與演化生物學
2. 海洋科學及海洋物理化學因子對微藻分佈的影響
3. 海洋微藻基因體變異,、轉錄體調控、生理特性對脂質生合成機制影響 ，以及對宿主-病毒交互作用關係。

Job description (Tasks are assigned flexibly; not all are required.)
-Culture and maintain microalgal and protist strains.
-DNA/RNA extraction, NGS libraries preparation, and perform sequencing.
-Design and conduct genomic, transcriptomic, or metabolomics experiments and analyses.
-Perform bioinformatic analyses, including genome assembly, annotation, and phylogenomic analysis, etc..
-May participate in field sampling during research cruises.
-Manage routine laboratory tasks and other assigned duties.

工作內容（彈性分配，非所有項目皆為必須）
-培養與維護微藻與原生生物菌株
-DNA/RNA抽取、NGS library建置與定序
-設計並執行基因體、轉錄體或代謝體實驗與數據分析
-生物資料分析，包括基因體組裝、註解、系統基因組學分析等
-研究航次期間，可能需參與現場採樣
-實驗室例行管理與其他交辦事項

-Master’s degree in biology, bioinformatics, data science, oceanography, or a related field.
-Relevant research experience related to the described topics and tasks.
-Self-motivated, able to work independently and collaboratively, with strong communication skills.
-Advanced proficiency in English writing and communication, including report writing and presenting research findings.
-Familiarity with Linux and programming languages is a plus.

-擁有生物學、生物資訊學、數據科學、海洋學或相關領域碩士學位
-具備與上述主題/技術相關之研究經驗
-積極主動，能獨立作業並具團隊合作與溝通能力
-具備良好英文書寫與表達能力，包括撰寫報告及發表研究成果
-熟悉 Linux 系統與程式語言者尤佳

Our institute is part of Academia Sinica, the Taiwanese academy of sciences. The working language in our lab and institute is English. Knowledge in Mandarin or other Taiwanese languages is not required, but foreign researchers are encouraged to take Mandarin classes if they want. More details can be found on our lab and institute websites. Inquiries are also welcome.

The Hsieh lab at IPMB focuses on fungal-bacterial competition (www.labofpie.org). We
combine functional genomics, genetics, and experimental evolution to determine the
mechanisms and consequences of such cross-kingdom competition.

Our lab will start in October 2025. We are seeking 1-2 M.S. level research associates to help establish our new lab. Candidates will have the opportunity to lead independent projects under Phoebe’s mentorship and earn authorship on resulting publications. This position is ideal for someone with microbiology and molecular biology experience who seeks more hands-on research training.

Responsibilities
• Basic microbiology and molecular biology experiments, including:
o Routine handling of bacterial and fungal culture
o High-throughput genetic screens
o Build gene knockout strains
o Molecular cloning, DNA extractions, and sequencing
• Lab management support, including media prep, ordering, and maintaining equipment
• Candidates are encouraged to lead and execute small-scale independent research projects
• Present data at the lab meeting and provide weekly research updates
• Weekly update on the experimental progress
• 2-year minimum appointment

Requirements:
• M.S. in biology, microbiology, or a related discipline
• Experience in molecular cloning and aseptic technique is highly desirable
• Ability to perform clean, contamination-free experiments
• Strong verbal and written communication skills
• Superior organizational, prioritization, and time management skills
• Accurate record-keeping and data documentation
• Ability to work as a team member
• Positive, adaptable, and self-motivated attitude

Preferred requirements:
• Highly organized and responsible
• Patient and strong interest in learning new experimental techniques