The goal of the Small Molecule Metabolomics core facility in the Institute of Plant and Microbial Biology is to provide cutting-edge mass spectrometry-based metabolomics services and cost-effective assays for investigators engaged in studying the metabolic perturbation of organisms. The Metabolomics Core offers a variety of services, including identification of targeted and untargeted metabolites, and global metabolites profiling. The Metabolomics Core is equipped with a liquid or gas chromatography coupled with mass spectrometry for separation and detection of various classes of metabolites. The metabolites we analyzed represent a diverse group of chemical compounds such as amino acids, lipids, carbohydrate, organic acids and environmental chemicals. We provide research consultation and technical supports like user trainings for instrument and software operations.

Services Offered

• Targeted qualitative and quantitative analysis
• Untargeted metabolomics analysis and principal component analysis
• Compound identification
• New methods development
• Instruments and software training course
• Experimental design and data consultation

Service Rates

• LC-MS: Academia Sinica Metabolomics Core Facility
• IPMB Metabolomics Core Equipment

Workflow

• LC-MS: Academia Sinica Metabolomics Core Facility
• IPMB Metabolomics Core Equipment
  1. Fill out the application form
  2. Consult with the manager
  3. Take a training course (4-8 Hours)
  4. Get an activated online account
  5. Book the instrument via the online reservation system
  6. Operate the open access instrument
  7. Analyze the data by the user

Reservation System

• LC-MS: Academia Sinica Metabolomics Core Facility
• IPMB Metabolomics Core Equipment

Training Course

• The schedule of training courses registration
• Contact manager to schedule an individual training

Instruments

Useful Links

Publications from Core users

1. C. Hsu, C. M. Chen, Y. M. Ju, Y. C. Wu, H. M. Hsieh, S. H. Yang, C. T. Su, T. C. Fang, W. Setyaningsih and S. C. Li. (2025) Effects of Consuming Pulsed UV Light-Treated Pleurotus citrinopileatus on Vitamin D Nutritional Status in Healthy Adults. Foods. 14(2)
2. W. Wang, R. H. Chen and Y. K. Chen. (2024) The lipid droplet assembly complex consists of seipin and four accessory factors in budding yeast. J Biol Chem. 300(8)107534
3. H. Ngo, Y. C. Wu and Y. Nakamura. (2024) Bidirectional movement of tunicamycin in Arabidopsis thaliana. New Phytol. 241(1)10-16
4. H. Chung, T. C. Chen, W. J. Yang, S. Z. Chen, J. M. Chang, W. Y. Hsieh and M. H. Hsieh. (2024) Ectopic expression of a bacterial thiamin monophosphate kinase enhances vitamin B1 biosynthesis in plants. Plant J. 117(5)1330-1343
5. J. Chen, C. H. Chou, T. H. Hsiao, T. Y. Wu, C. Y. Li, Y. L. Chen, K. H. Chao, T. H. Lee, R. G. Gicana, C. J. Shih, G. J. Brandon-Mong, Y. L. Lai, P. T. Li, Y. L. Tseng, P. H. Wang and Y. R. Chiang. (2024) Clostridium innocuum, an opportunistic gut pathogen, inactivates host gut progesterone and arrests ovarian follicular development. Gut Microbes. 16(1)2424911
6. Anggarani, Y. Y. Lin, S. A. Fang, H. P. Wu, C. C. Wu, W. N. Jane, T. J. Roscoe, F. Domergue and Y. C. Hsing. (2024) Morphology and chemical composition of Taiwan oil millet (Eccoilopus formosanus) epicuticular wax. Planta. 259(4)89
7. H. Lin, M. Y. Xu, C. C. Hsu, F. A. Damei, H. C. Lee, W. L. Tsai, C. V. Hoang, Y. R. Chiang and L. S. Ma. (2023) Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities. Nat Commun. 14(1)5755
8. J. Huang and R. H. Chen. (2023) Lipid saturation induces degradation of squalene epoxidase for sterol homeostasis and cell survival. Life Sci Alliance. 6(1)
9. Y. Hsieh, H. M. Wang, Y. H. Chung, K. T. Lee, H. S. Liao and M. H. Hsieh. (2022) THIAMIN REQUIRING2 is involved in thiamin diphosphate biosynthesis and homeostasis. Plant J. 111(5)1383-1396
10. J. Hsieh, W. D. Lin and W. Schmidt. (2022) Genomically Hardwired Regulation of Gene Activity Orchestrates Cellular Iron Homeostasis in Arabidopsis. RNA Biol. 19(1)143-161
11. H. Chen, H. L. Shen, S. J. Chou, Y. Sato and W. H. Cheng. (2022) Interference of Arabidopsis N-Acetylglucosamine-1-P Uridylyltransferase Expression Impairs Protein N-Glycosylation and Induces ABA-Mediated Salt Sensitivity During Seed Germination and Early Seedling Development. Front Plant Sci. 13()903272