Targeted qualitative and Quantitative analysis

Established Methods for Metabolites-LCMS

Amino Acids

• Amino Acid：
  Tryptophan/ Phenylalanine/ Isoleucine/ Leucine/ Methionine/ Tyrosine/ Valine/ Proline/ Cysteine/ Alanine/ Glutamate/ Threonine/ Homocysteine/ Glycine/ Glutamine/ Serine/ Asparagine/ Aspartate/ Sarcosine/ Arginine/ Histidine/ Lysine/ Cystine / Homocysteine/ Sarcosine/ S-solfocysteine
• Polyamine：
  Spermidine/ Spermine/ Putrescine/ Ethanolamine/ Diaminohexane/ Cadaverine/ 1,3-Diaminopropane/ N1-Acetylspermidine/ N1,N12-Diacetylspermine/ N-Acetylputrescine/ N1,N8-Diacetylspermidine

Carbohydrates

• Glycolysis：
  Glucose/ G6P/ F6P/ F1,6BP/ GADP/DHAP/3PG/2PG/ PEP/ Pyruvate/ Lactate/ Gln6P
• TCA cycle：
  Acetyl-CoA/ Citrate/ Aconitate/ Isocitrate/ α-KG/ Succinyl-CoA/ Succinate/ Fumarate/ Malate/ OAA
• Pentose Phosphate Pathway：
  R5P/E4P/ S7P/ G3P/ Ru5P/ X5P/ RuBP/ G1P

Cofactors and Vitamins

• Vitamin：
  B1/B2/B3/B4/B5/Pyridoxal/Pyridoxine/B7/B9/B12/ Choline/ B10/ TMP/ TPP/ C/ FAD/ FMN
• Cofactor：
  AMP/NADPH/ NADP+/ CoA/ NADH/ NAD+

Energy

• ADP/ATP

Lipids

• Fatty Acids
• Phospholipids
• Steroids：
  Testosterone/1-Dehydrotestosteraone/ Androstenedione/ Boldione/ 4-BNC/ 25-Hydroxycholesterol/ Cholesterol/ Progesterone/ Lanosterol/ Cortisol/ Estradiol
• Prostaglandin：
  PGF2a/ PGE2/ 15 keto PGF2a/ 15 Keto PGE2/ 13,14-DiH 15 PGF2a/ 13,14-DiH15 ketoPGE2/ Leukotriene B4/ 15d PGJ2/ 5 HEPE/ 13 HODE/ 9 HODE/ 15S HETE/ 13 OxoODE/ 15 OxoETE/ 9 oxoODE/ 5 HETE/ 4 HDHA/ 5 oxoETE/ Linoleic acid/ NAGly
• Fatty Acyl Carnitine：
  Decanoyl carnitine/Lauroyl carnitine/Myristoyl carnitine/ Linoleoyl carnitine/ Palmitoyl carnitine/ Oleoyl carnitine/ Stearyoyl carnitine/ Octanoyl carnitine/Hexanoyl carnitine/Isovaleryl carnitine/Butyryl carnitine/Propionyl carnitine/Acetyl carnitine/ Carnitine
• Fatty Acyl CoA：
  Palmitoleoyl CoA/Palmitoyl CoA/Linoleoyl CoA/Oleoyl CoA/Stearoyl CoA/Arachidonoyl CoA
• Short Chain Fatty Acid：
  Butyric acid/Valeric acid/Phenylacetic acid/Heptanoic acid/ Sphingosine/ Ceramide/ Sphingomyelin
• Cholesterol Synthesis :
  Acetoacetyl-CoA/ Mevalonate/ HMG-CoA/ Lanosterol/ Cholesterol/Desmosterol/Squalene

Nucleotides

• Nucleotide Sugar：
  UDP-glucose/ ADP-glucose/ UDP-GlcNAc/ GDP-Glc-NAc
• Nucleotide：
  ATP/CTP/GTP/UTP/dGTP/dATP/dCTP/dUTP
• Nucleobase：
  Adenine/ Thymine/ Cycosine/ Guanine/ Methylcytosine
• Signal Molecules：
  cAMP/ cGMP/ c-di-AMP/ c-di GMP

Plant Metabolites

• Carotenes：
  Carotene/ Astaxanthin/ Canthaxannthin/ Zeaxanthin/ Lutein
• Coumarins：
  Scopoletin/ Fraxetin/ Esculetin/ Esculin/ 4-Methyl-umbelliferone
• Cytokinins：
  Zeantin/ 6-Dimethylallyamino-purine/ Zeantin riboside/ Thidiazuron/ Kinetin
• Flavonoids：
  Apigenin/ Luteolin/ Wedelolactone/ Quercetin/ Kaempferol
• Ginsenosides：
  3Rg3/ CK/ Rh2
• Glucosinolates：
  4MSOB/ 5MSOP/ 6MSOH/ 7MSOH/ 8MSOO/ 4MTB
• Nicotines：
  Nicotine/ Nornicotine/ Anabasine/ Anatabine
• Phytohormones：
  SA/ IAA/ ABA/ JA/ JA-Ile/ GA3/ MeJA
• Phytosiderophores：
  NA/ DMA/ HDMA/ HMA/ AVA/ HAVACytokinins

• N1-Acetylspermidine/ N1,N12-Diacetylspermine/ N-Acetylputrescine/ N1,N8-Diacetylspermidine