Cheng, Wan-Hsing (鄭萬興)
Associate Research Fellow
- Ph.D. Plant Molecular and Cellular Biology Program University of Florida
- M.S. Horticulture National Taiwan University
- B.S. Horticulture National Chung-Hsing University
- Abscisic acid, abiotic stress, and their interaction
Abscisic acid, abiotic stress, and their interaction
The plant hormone abscisic acid (ABA) plays a pivotal role in regulating seed development, dormancy, stomatal closure, and stress tolerance (Fig. A). ABA also affects heterophylly, different types of leaf in a plant, in the lily (a geophytic plant) through differential expression of 9-cis-epoxycarotenoid dioxygenase 3 (NCED3), a key gene in ABA biosynthesis. Moreover, antagonism between ABA vs. ethylene and ABA vs. GA affects seed germination, seedling growth, and leaf development. These hormones may control the hormonal biosynthesis, catabolism, or signaling of each other to enhance their antagonism.
For study of abiotic stress, ten salt hypersensitive (sahy) mutants (Fig. B) were isolated by screening T-DNA tagged seeds on high salt-containing media. Of which, both sahy1 and sahy9 revealed strong salt oversensitivity and plant growth defect, including small plant size, short root, and pointed leaves. These two proteins are mainly localized to the nucleolus and have roles in ribosomal biogenesis/assembly, which might change ribosome composition and cause differential or preferential translation. They showed auxin-mediated developmental defects in leaf vascular pattern, arrangement of palisade mesophyll cells, and auxin transport and response. In addition, they also affected salt sensitivity at least through ABA-dependent, ABA-independent, and salt-responsive gene expression.
To further dissect the components involved in ABA signal pathway and crosstalk with stress signal pathways, we have isolated several aba2 suppressor (abs) mutants (Fig. C) based on their response to high glucose and salt sensitivity. These abs mutants with ABA deficiency showed different soil-grown phenotypes from aba2 and wild type. This study will provide novel mechanisms by which ABA crosstalks to nutrient and salt stress signaling.
Selected publication list
- Hsu P, Tan MC, Shen HL, Chen YH, Wang YY, Hwang SG, Chiang MH, Le QV, Kuo WS, Chou YC, Lin SY, Jauh GY, Cheng WH* (2020) The nucleolar protein SAHY1 is involved in pre-rRNA processing and normal plant growth. Plant Physiol (in press, DOI: 10.1093/plphys/kiaa085)
- Chen HC, Cheng WH, Hong CY, Chang YS, Chang MC* (2018) The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively. Rice 11: 50.
- Huang KC, Lin WC, Cheng WH* (2018) Salt hypersensitive mutant 9, a nucleolar APUM23 protein, is essential for salt sensitivity in association with the ABA signaling pathway in Arabidopsis. BMC Plant Biology 18: 40.
- Chen HC, Hsieh‑Feng V, Liao PC, Cheng WH, Liu LY, Yang YW, Lai MH, Chang MC* (2017) The function of OsbHLH068 is partially redundant with its homolog, AtbHLH112, in the regulation of the salt stress response but has opposite functions to control flowering in Arabidopsis. Plant Mol Biol 94:531–548.
- Chiang MH, Shen HL, Cheng WH* (2015) Genetic analyses of the interaction between abscisic acid and gibberellins in the control of leaf development in Arabidopsis thaliana. Plant Science 236: 260-271.
- Chen YH, Shen HL, Hsu PJ, Hwang SG, Cheng WH* (2014) N-acetylglucosamine-1-P uridylytransferase 1 and 2 are required for gametogenesis and embryo development in Arabidopsis thaliana. Plant Cell Physiol. 55:1977-1993.
- Hsu YF, Chen YC, Hsiao YC, Wang BJ, Lin SY, Cheng WH, Jauh GY, Harada JJ, Wang CS* (2014) AtRH57, a DEAD RNA helicase, is involved in feedback inhibition of glucose-mediated abscisic acid accumulation during seedling development and additively affects pre-ribosomal RNA processing with high glucose. Plant J. 77:119-135.
- Hwang SG, Lin NC, Hsiao YY, Kuo CH, Chang PF, Deng WL, Chiang MH, Shen HL, Chen CY, Cheng WH* (2012) The Arabidopsis short-chain dehydrogenase/reductase 3, an ABSCISIC ACID DEFFICIENT 2 homolog, is involved in plant defense responses but not in ABA biosynthesis. Plant Physiol. Biochem. 51:63-73.
- Chen HC, Hwang SG, Chen SM, Shii CT, Cheng WH* (2011) ABA-mediated heterophylly is regulated by differential expression of 9-cis-expoxycarotenoid dioxygenase 3 in lilies. Plant Cell Physiol. 52:1806-1821.