The lab of Ka Wai Ma is inviting application working on plant-
microbiota research. The lab is using different model systems and the
associated culture collection to understand the principles governing
plant-microbiota homeostasis. The lab routinely uses omics
techniques including amplicon sequencing, transcriptomics and
reconstitution system. The lab is currently looking for a motivated
candidate to work on a CRISPR editing project. The project aims to
use CRISPR technology to perform gene editing on crops with
subsequent phenotyping.
The position is opened until filled
馬家威實驗室現誠聘研究助理/博士後，從事植物微生物相研究。本實
驗室使用不同的模型系統和相關的微生物庫進行植物微生物相重組實驗
以了解植物微生物群互動平衡機理。實驗室大量使用比如包括擴增子測
序、轉錄組學技術。本實驗室目前正在尋找助理/博士後從事 CRISPR
編輯專案。該專案旨在使用 CRISPR 技術對作物進行基因編輯，並進
行表型分析。

Applicants are required to

1. hold a MS degree (RA) or a PhD (postdoc)
2. have research experience in molecular biology and CRISPR
technology
3. experience in the use of golden gate cloning system, Gibson
4. working experience on monocot/crops are preferred
5. Good command of English and preferably basic knowledge of
mandarin.
申請者要求
6. 具碩士學位 (研究助理) 或博士學位 (博士後)
7. 具份子生物學及CRISPR基因編輯經驗，無相關經驗者不作考慮
8. 曾使用golden gate 及 Gibson 系統者為佳
9. 具農作物相關操作經驗者優先考慮
10. 具英語交流書寫能力，通華語為佳 (非必要)

參與 (1)台灣新穎的海洋溫泉藍綠菌株的光合作用和適應環境的分子機制，比較基因體分析與新型的抗生素生合成基因的探討。

(2)台灣新穎的溫泉藍綠菌變異株的趨光性移動和胞外多醣生合成的分子機制探討。

The lab is using moss (Physcomitrium patens) as a model to
investigate various developmental biology-related questions
in plants. The lab routinely employs techniques such as
genetic manipulations, protein expression/purification, and
confocal microscopy observation. We encourage those
interested in plant biology to be part of the team.
實驗室網站：https://sites.google.com/view/kock-lab/home

(1) A PhD degree in Plant Biology/Genetics/Biochemistry or a
related discipline.
(2) A good command of English and capable of acquiring new
skills.
(3) Must be experienced in more than one of the following
techniques: (1) molecular biology (Golden
gate/gateway/Gibson assembly), confocal microscopy (time-
lapse imaging, image analysis), DNA sequencing analysis
(RNAseq, scRNAseq), Biomolecular interactions (in vitro pull-
down, MicroScale Thermophoresis, Isothermal titration
calorimetry).
(4) A good track record of publication that demonstrates the
above.

Two potential research areas are:

1. Giant viruses: evolution of viral gigantism, genome regulation, and virus-host interactions

Giant viruses are DNA viruses with extraordinary genomes that are associated with a wide range of eukaryotic hosts, including animals, fungi, amoebae, algae, and many other microeukaryotes.

2. Phages: evolution of phage resistance and susceptibility and dynamic phage-bacteria interactions

We focus on phages that infect antibiotic-resistant bacteria to study what determines the outcome of phage infection and to apply this knowledge to develop broad-range phages.

1. A PhD degree in biology, informatics, natural sciences or related fields
2. English writing and speaking skills
3. Ability to work both independently and as part of a team
4. Experience and knowledge in genomics, bioinformatics, evolutionary biology or microbial ecology

Our institute is part of Academia Sinica, the Taiwanese academy of sciences. The working language in our lab and institute is English. Knowledge in Mandarin or other Taiwanese languages is not required, but foreign researchers are encouraged to take Mandarin classes if they want. More details can be found on our lab and institute websites.

Our research team is seeking 1 or 2 master’s-level Research Associates to join us in exploring the evolution, ecology and biology of eukaryotic microbes with key roles on Earth. We focus on the interactions between microbes and their environment and utilize cutting-edge omics and bioinformatics tools to uncover their evolutionary trajectories and regulatory mechanisms. The position is opened until filled.

顧銓研究室誠徵1 至 2 名碩士級研究助理，一同探索在地球上扮演重要角色的真核微生物之演化、生態與生物學。我們專注於微生物與環境的互動，並運用最先進的體學與生物資訊工具，以發掘其演化軌跡與調控機制。該職缺將開放至額滿為止。

Job description (at least three of the tasks listed below):

- Culturing and maintaining microalgal and protist strains

- Nucleic acid extraction, NGS library preparation and sequencing

- Single-cell omics analysis

- Bioinformatic analyses, including genome assembly, annotation, phylogenomic analysis, etc.

- Giant virus isolation and characterization

- Other routine laboratory affairs and assigned tasks

工作內容（至少三項任務）：

- 培養與維持微藻及原生生物菌株

- 核酸萃取、NGS建庫與定序

- 單細胞體學分析

- 生物資訊分析，包括基因體組裝、註解、親緣基因體學分析等

- 巨病毒分離與特性分析

- 其他實驗室例行事務與交辦事項

1. Master’s degree in biology or a related field
2. Research experience related to topics and tasks in our lab
3. Proficient English communication skills
4. Active participation in discussion, report writing, and dissemination of research findings
5. Familiarity with Linux and programming is a plus

1. 具生物學或相關領域之碩士學歷
2. 與本實驗室研究主題相關之工作或研究經驗
3. 良好的英語溝通能力
4. 能積極參與討論、撰寫報告，並發表研究成果
5. 熟悉 Linux 與程式語言者尤佳