研究助理

中央研究院植物暨微生物學研究所（IPMB）吳亭穎博士實驗室誠徵全職研究助理。

本實驗室位於臺北市中央研究院院本部。植物暨微生物學研究所匯集近 300 名來自臺灣及其他 15 個以上國家的研究人員與工作人員，並設有細胞生物學、顯微影像、高效能運算及多體學分析等先進核心設施，提供完善的跨領域研究環境。實驗室重視團隊合作與跨領域交流，並與世界各地的研究團隊維持密切合作。

錄取者將參與植物與環境交互作用相關研究，主要以地錢（Marchantia）、阿拉伯芥（Arabidopsis）及可能包含大豆等植物為研究材料，探討植物在熱逆境及全球暖化相關環境變化下的生理與分子反應，以及高海拔作物生理時鐘受環境變化影響的調控機制。主要工作內容包括分子選殖、蛋白質表現分析及突變株篩選。

本研究計畫將與比利時 Ive De Smet 博士及 Devang Mehta 博士的研究團隊合作進行。

• 具生物學或相關領域碩士學位

• 具分子生物學及植物生物學相關研究經驗

• 具良好的英文口語及書面溝通能力

• 能積極參與科學討論及研究報告撰寫

計時工讀生一名

1.實驗室例行事項準備，例如: 補充tip、滅菌、 清洗實驗器皿、實驗器皿歸位、實驗室清潔…等。

2.配合實驗室需求之支援性工作

3.工作時間彈性，以一週為六到八小時為主

工作細心有條理,無經驗可 (有實驗室經驗與願意長期配合尤佳)

The major research interests in the LAI lab are to understand the molecular mechanisms underlying how bacterial pathogens deploy protein secretion systems for survival at its ecological niche and successful infection inside the plant host. We study the mechanistic and biological insights of the two major protein secretion nanomachines, type IV and type VI secretion systems (T4SS and T6SS), in Agrobacterium tumefaciens, the causal agent of crown gall disease and powerful gene transfer tool. We also aim to identify novel agrobacterial and plant genes critical for plant transformation and gene editing. The knowledge may provide new insights to improve Agrobacterium-mediated plant transformation and translational applications in biomedicine and/or agriculture.

The postdoc will carry out a two-year research grant to study the antibacterial T6SS with focuses to investigate the T6SS evolution and assembly mechanisms.

The LAI lab provides the research environments and expertise on studies of protein secretion and pathogen-plant interactions using various tools including genetics/genomics, molecular biology, biochemistry/proteomics, and cell biology. The research may expand to study structural aspects of T6SS-assocaited protein complex using X-ray crystallography and cryoEM. The postdoc is also encouraged to develop an independent project and will be trained to serve as a mentor toward an independent investigator. The LAI lab is also active in international collaborations, which will bring opportunities for abroad training.

對微生物學研究具有高度熱忱，並至少具備下列兩項專長或研究經驗者：細菌遺傳學、蛋白質化學、電子顯微鏡技術或生物資訊分析。

研究助理

The major research interests in the LAI lab are to understand the molecular mechanisms underlying how bacterial pathogens deploy protein secretion systems for survival at its ecological niche and successful infection inside the plant host. We study the mechanistic and biological insights of the two major protein secretion nanomachines, type IV and type VI secretion systems (T4SS and T6SS), in Agrobacterium tumefaciens, the causal agent of crown gall disease and powerful gene transfer tool. We also aim to identify novel agrobacterial and plant genes critical for plant transformation and gene editing. The knowledge may provide new insights to improve Agrobacterium-mediated plant transformation and translational applications in biomedicine and/or agriculture.

The Research Assistant will work with a senior Ph.D. student or a postdoc to carry out a two-year research grant to study the antibacterial T6SS with focuses to investigate the T6SS evolution and assembly mechanisms.

The LAI lab provides the research environments and expertise on studies of protein secretion and pathogen-plant interactions using various tools including genetics/genomics, molecular biology, biochemistry/proteomics, and cell biology. The research may expand to study structural aspects of T6SS-assocaited protein complex using X-ray crystallography and cryoEM. The postdoc is also encouraged to develop an independent project and will be trained to serve as a mentor toward an independent investigator. The LAI lab is also active in international collaborations, which will bring opportunities for abroad training.

碩士或學士，對微生物學研究具有高度熱忱，具備下列專長或研究經驗者尤佳：細菌遺傳學、蛋白質化學、電子顯微鏡技術或生物資訊分析。

申請人應具有近期獲得之碩士或學士學位，並具備研究經驗。

具分子生物學、生物化學或細胞生物學經驗者優先。

植物生理學和植物照顧經驗者優先，但非強制要求。

本計畫旨在闡明轉錄因子（transcription factors）在植物多種細胞分化過程中的功能與演化。錄取者將以阿拉伯芥（Arabidopsis thaliana）與本氏菸草（Nicotiana benthamiana）為主要模式植物進行濕實驗（wet-lab experiments），工作內容包括：植物栽種、分子選殖（molecular cloning）、植物轉殖（plant transformation）、顯微鏡分析（microscopy-based analyses）以及暫時性表現分析（transient reporter assays）。此外，錄取者須確實記錄實驗數據，並能使用 Microsoft Excel 進行基本的數據整理與統計分析。

1. 學歷要求：須具備生命科學、植物生物學、分子生物學、生物技術或相關領域之碩士學位。
2. 研究經驗：須具備至少一年以上操作阿拉伯芥（Arabidopsis）及／或本氏菸草（Nicotiana benthamiana）的實際研究經驗。具備兩年以上經驗者將優先考慮。
3. 數據處理能力：須具備基本數據管理與 Excel 技能，能熟練使用 Microsoft Excel 整理實驗數據並進行基礎統計分析。
4. 英文能力：須具備基礎英文溝通能力，能於團體會議（Lab meeting）中以英文進行交流，並能流暢閱讀與理解英文實驗通則（Protocols）。

The lab is using moss (Physcomitrium patens) as a model to investigate various developmental biology-related questions in plants. The lab routinely employs techniques such as genetic manipulations, protein expression/purification, and confocal microscopy observation. We encourage those interested in plant biology to be part of the team.

• A PhD degree in Plant Biology/Genetics/Biochemistry or a related discipline.
• A good command of English and capable of acquiring new skills.
• Must be experienced in more than one of the following techniques: (1) molecular biology (Golden gate/gateway/Gibson assembly), confocal microscopy (time-lapse imaging, image analysis), DNA sequencing analysis (RNAseq, scRNAseq), Biomolecular interactions (in vitro pull-down, MicroScale Thermophoresis, Isothermal titration calorimetry).
• A good track record of publication that demonstrates the above.

本實驗室利用蘚苔為實驗材料，致力於研究陸生植物的外表形態環境適應及其訊號傳遞的演化過程機制。本計畫會用到分子生物、遺傳、基因組編輯、顯微鏡觀察及蛋白純化等技術。歡迎對植物研究工作有熱忱的你加入。The lab is using moss (Physcomitrium patens) as a model to investigate various developmental biology-related questions in plants. The lab routinely employs techniques such as genetic manipulations, protein expression/purification, and confocal microscopy observation. We encourage those interested in plant biology to be part of the team.

（1）具備並熟悉以下其中一項技術：Molecular biology (Golden gate/gateway/Gibson assembly), confocal microscopy (time-lapse imaging, image analysis), DNA sequencing analysis (RNAseq), Biomolecular interactions (in vitro pull-down, MicroScale Thermophoresis, Isothermal titration calorimetry).

（2）中英文能力佳（實驗室會議及實驗紀錄皆使用英文）A  good command of English and Mandarin；

（3）生命科學等相關學位 Possess a life science-related degree in the fields of plant and/cell biology/genetics ；

（4）外籍人士也歡迎。International applicants are encouraged to apply.

We are seeking 1–2 postdoctoral fellows to join our lab in studying polymicrobial interactions and host–microbe interactions with agricultural relevance. Our research integrates comparative genomics, ecological sampling, microbial genetics, and functional assays to understand how fungi, bacteria, and phages interact within polymicrobial communities and how these interactions influence microbial pathogenesis in plant hosts.

Our lab has strong expertise in functional genomics and experimental evolution, using bottom-up approaches to address these questions. We welcome postdoctoral researchers with complementary interests—such as plant immunity, evolutionary genomics, or microbial ecology—to join our team. This position is ideal for recently graduated PhD scientists who are passionate about microbial interactions and aspire to pursue a faculty position in academia.

The position is open until filled.
Lab website: www.labofpie.org

Research Topics

1. Genetic basis of fungal–bacterial–phage interactions and their impact on microbial community development
2. Comparative analysis of microbial pathogenic genes in single versus mixed infections
3. Bioinformatic and evolutionary analysis of antimicrobial genes in plant pathogens

Job Responsibilities

1. Lead and contribute to independent and collaborative research projects
2. Design and conduct experiments and/or computational data analyses
3. Develop and maintain standardized laboratory protocols or bioinformatic pipelines
4. Actively participate in manuscript preparation and scientific presentations
5. Mentor graduate and undergraduate researchers

• PhD in biology, microbiology, genomics, evolutionary biology, or a related discipline
• Experience in phage biology, plant immunity, comparative genomics, and/or working with plant fungal or bacterial pathogens is highly desirable
• Strong programming skills in Python and R, with experience analyzing NGS data, preferred
• Ability to work independently as well as collaboratively in a team environment
• Excellent English writing and communication skills
• Willingness to mentor students and develop grant-writing skills
• Strong organizational, prioritization, and time-management abilities
• Positive, adaptable, and self-motivated attitude

We are seeking 1-2 M.S.-level research associates to join us in dissecting the mechanisms and consequences of fungal-bacterial competition. We focus on the interaction between Pseudomonas species and fungal plant pathogens. Using ecological, functional genomic, and genetic approaches, we aim to determine whether bacterial defense mechanisms are specific or general against multiple fungi, and vice versa. Check our lab website: www.labofpie.org

Research topics

1. Functional genomic investigation of fungal or bacterial defense genes
2. Ecology and sampling of Pseudomonas species and interacting fungi in the wild
3. Co-evolutionary consequences of competing fungi and bacteria

Job description:

1. Lead and execute a small-scale independent research project.
2. Present data at the lab meeting and provide weekly research updates
3. Participate in joint lab meetings and journal clubs

This position is ideal for someone who seeks graduate-level training and have strong interest in microbial ecology and genetics. This position is open until filled

• M.S. in biology, microbiology, or a related discipline
• Experience in molecular cloning and microbial genetics is highly desirable
• Experience in working with plant-related pathogens is favored
• Ability to perform clean, contamination-free experiments
• Strong verbal and written communication skills
• Superior organizational, prioritization, and time management skills
• Accurate record-keeping and data documentation
• Ability to work as a team member
• Positive, adaptable, and self-motivated attitude

Preferred requirements:

• Highly organized and responsible
• Patience, independence, and strong interest in exploring novel biology

Our research team is seeking a Postdoctoral Researcher to join us in exploring the evolution, ecology, and biology of eukaryotic microbes with key ecological roles on Earth. We focus on the interactions between microbes and their environment and utilize cutting-edge omics and bioinformatics tools to uncover their evolutionary trajectories and regulatory mechanisms. Currently, we are conducting an NSTC-funded project on marine algae, population variation, and warming. This position is open until filled.

Research Topics
1. Population genomics and evolutionary biology of marine algae.
2. Effects of oceanographic and physico-chemical factors on algal distribution.
3. Genomic variation, transcriptomic regulation and physiological properties of lipid synthesis and host-virus interactions in marine algae.

Job description:
1. Lead and participate in research projects.
2. Design and conduct experiments or data analyses.
3. Establish standardized protocols or pipelines for all team members.
4. Actively participate in scientific writing and presentation
5. Mentor junior researchers

-PhD in genomics, bioinformatics, evolutionary biology, marine biology, microbiology, or a related field.
-Research experience in related topics.
-Strong programming skills in Python, R, or other relevant languages.
-Ability to lead research projects independently and collaborate effectively within a team.
-Proficient in English writing and communication skills.
-Experience in writing scientific papers and presenting at conferences.

Our institute is part of Academia Sinica, the Taiwanese academy of sciences. The working language in our lab and institute is English. Knowledge in Mandarin or other Taiwanese languages is not required, but foreign researchers are encouraged to take Mandarin classes if they want. More details can be found on our lab and institute websites. Inquiries are also welcome.

Our research team is seeking 1~2 Research Associates to join us in exploring the evolution, ecology, and biology of eukaryotic microbes with key ecological roles on Earth. We focus on the interactions between microbes and their environment and utilize cutting-edge omics and bioinformatics tools to uncover their evolutionary trajectories and regulatory mechanisms. Currently, we are conducting an NSTC-funded project on marine algae, population variation, and warming. This position is open until filled.

顧銓研究室現正招募 1 至 2 位研究助理，加入我們探索真核微生物之演化、生態與生物學上的關鍵生態角色。我們著重微生物與環境之間的互動，並利用尖端的多體學與生物資訊工具發掘演化軌跡與調控機制。目前我們正執行國科會(NSTC)資助的計畫，研究海藻族群變異與暖化相關議題。本職缺即日起開放申請，額滿為止。

Research Topics
1. Population genomics and evolutionary biology of marine algae.
2. Effects of oceanographic and physico-chemical factors on algal distribution.
3. Genomic variation, transcriptomic regulation and physiological properties of lipid synthesis and host-virus interactions in marine algae.

研究主題
1. 海洋微藻族群基因體學與演化生物學
2. 海洋科學及海洋物理化學因子對微藻分佈的影響
3. 海洋微藻基因體變異,、轉錄體調控、生理特性對脂質生合成機制影響 ，以及對宿主-病毒交互作用關係。

Job description (Tasks are assigned flexibly; not all are required.)
-Culture and maintain microalgal and protist strains.
-DNA/RNA extraction, NGS libraries preparation, and perform sequencing.
-Design and conduct genomic, transcriptomic, or metabolomics experiments and analyses.
-Perform bioinformatic analyses, including genome assembly, annotation, and phylogenomic analysis, etc..
-May participate in field sampling during research cruises.
-Manage routine laboratory tasks and other assigned duties.

工作內容（彈性分配，非所有項目皆為必須）
-培養與維護微藻與原生生物菌株
-DNA/RNA抽取、NGS library建置與定序
-設計並執行基因體、轉錄體或代謝體實驗與數據分析
-生物資料分析，包括基因體組裝、註解、系統基因組學分析等
-研究航次期間，可能需參與現場採樣
-實驗室例行管理與其他交辦事項

-Master’s degree in biology, bioinformatics, data science, oceanography, or a related field.
-Relevant research experience related to the described topics and tasks.
-Self-motivated, able to work independently and collaboratively, with strong communication skills.
-Advanced proficiency in English writing and communication, including report writing and presenting research findings.
-Familiarity with Linux and programming languages is a plus.

-擁有生物學、生物資訊學、數據科學、海洋學或相關領域碩士學位
-具備與上述主題/技術相關之研究經驗
-積極主動，能獨立作業並具團隊合作與溝通能力
-具備良好英文書寫與表達能力，包括撰寫報告及發表研究成果
-熟悉 Linux 系統與程式語言者尤佳

Our institute is part of Academia Sinica, the Taiwanese academy of sciences. The working language in our lab and institute is English. Knowledge in Mandarin or other Taiwanese languages is not required, but foreign researchers are encouraged to take Mandarin classes if they want. More details can be found on our lab and institute websites. Inquiries are also welcome.