With the rapid development of High-Throughput Sequencing (HTS) technologies, Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) have effectively replaced traditional microarrays, becoming the gold standard for genomics and transcriptomics research. Currently, they are widely applied in applications including gene expression profiling, small RNA analysis, ChIP sequencing, SNP detection, and whole-genome sequencing of novel species.

Recognizing that “a high-quality sequencing library is the absolute key to obtaining reliable sequencing data,” our division provides standardized and customized library preparation services across both NGS and TGS platforms to suit diverse applications, while continuously investing in the development of novel library construction methodologies.

To maximize research efficiency, G-TeC has established stable collaborations with the NGS core in Academia Sinica and leading commercial sequencing companies. The high-quality libraries generated by our division can be directly submitted to these facilities for sequencing. This not only effectively shortens data turnaround time but also allows researchers to benefit from economical, convenient, and highly efficient services that keep pace with the latest sequencing technologies.

Current Services

1. High-Throughput Sequencing (HTS) Library Preparation Services

► Illumina Sequencing Platform (Library Preparation)

• Genomic DNA-seq
• ChIP-seq
• DAP-seq (DNA Affinity Purification sequencing)
• EM-seq (Enzymatic Methyl-seq)
• Microbiome DNA enrichment DNA-seq
• RNA-seq (poly-A enrichment or rRNA depletion)
• Small/miRNA-seq
• Parallel Analysis of RNA Ends (PARE)
• Ribosome Footprinting / Ribo-seq
• PAT-seq (PolyA-Tag seq.)
• 16S/ITS amplicon
• Ultra-low input RNA-seq (> 10 pg RNA)
• Custom NGS library design and construction
• Library QC and multiplex pooling

► Oxford Nanopore Technologies (ONT) Platform (Library Construction & Sequencing)

• Genomic DNA and native barcoding sequencing
• Rapid barcoding small genome sequencing
• Direct RNA sequencing
• Direct cDNA barcoding sequencing
• cDNA-PCR barcoding sequencing

► 10x Genomics Chromium X Platform (Single-Cell / Single-Nucleus Library Preparation)

• Single-cell / Single-nucleus RNA-seq
• Single-nucleus ATAC-seq

2. Droplet Digital PCR & Nucleic Acid QC

► Droplet Digital PCR (ddPCR): Utilizing the QX600 System for absolute nucleic acid quantification, this service is highly ideal for low-abundance gene expression detection, copy number variation (CNV) analysis, and rare mutation detection.

► Nucleic Acid Quality Analysis: Providing Agilent Bioanalyzer, Agilent Fragment Analyzer, and Qubit assay services for precise evaluation of nucleic acid concentration and integrity.

Key Equipment

• Agilent Bioanalyzer
• Agilent Fragment Analyzer
• NanoPhotometer NP80
• Qubit 4.0
• Covaris M220 Focused ultrasonicator
• Sage Science BluePippin DNA size selection
• 10x Genomics Chromium X
• BioRad droplet digital PCR QX600 system
• PCR machines

Sample Submission & Inquiries

To ensure the highest quality of sample processing and optimal analysis results, please contact us in advance to schedule your experiments prior to submitting samples. For service submission forms, please refer to the following:

• IPMB Users: Please log in to the IPMB Intranet to download the required submission forms.
• Non-IPMB Users: For service details, form requests, and project collaborations, please directly contact our Core Facility Manager, Shu-Jen Chou (Email: sjchou@gate.sinica.edu.tw; Tel.: 02-27871072).

Instruments