簡萬能 (Jane, Wann-Neng)


  • +886-2-2787-1012(Office)
  • wnjane@gate.sinica.edu.tw
  • 植物細胞學核心實驗室

我的工作是負責細胞學核心實驗室的運作。多年來我們建立各式電子顯微鏡的相關技術,包括超薄切片、負染色、金屬投影、冷凍置換、冷凍蝕刻、和免疫定位等等。近年來,更成功建立了冷凍掃描式電子顯微鏡和高壓冷凍結合冷凍置換兩套新技術。我的工作內容包括了:維持各項儀器的正常運作,並協助使用者使用; 發展與植物細胞學研究相關的技術,尤其是冷凍方面的技術;提供樣品製備、切片、染色、免疫定位等服務;提供諮詢,根據研究目的,幫助研究者選擇最適當的方法;訓練新的使用者使用各項儀器與技術。

Selected publication list

  • Wang HJ, Hsu YW, Guo CL, Jane WN, Wang H, Jiang L, Jauh GY* (2017) VPS36-Mediated MVB Formation is Critical for Plasma- membrane Protein Turnover and Vacuolar Biogenesis. Plant Physiol. 173:566-581.
  • Huang XC, Inoue-Aono Y, Moriyasu Y, Hsieh PY, Tu WM, Hsiao SC, Jane WN, Hsu HY* (2016) Plant Cell Wall-Penetrable, Redox-Responsive Silica Nanoprobe for the Imaging of Starvation-Induced Vesicle Trafficking. Anal. Chem. 88:10231-10236.
  • Wang WJ, Acehan D, Kao CH, Jane WN, Uryu K, Bryan Tsou MF* (2015) De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly. eLife 4:e10586 DOI: 10.7554.
  • Li, PS, Lee IL, Yu WL, Sun JS, Jane WN, Shen HH* (2014) A novel albumin-based tissue scaffold for autogenic tissue engineering applications. Scientific Reports 4:5600 DOI: 10.1038/srep05600
  • Liu, MC, Yang CS, Yeh FL, Wei CH, Jane WN, Chung MC, Wang CS* (2014) A novel lily anther-specific gene encodes adhesin-like proteins associated with exine formation during anther development. J. Exp. Bot. 65:2023-2037.
  • Chen, PJ, Senthilkumar R, Jane WN, Tian ZH, Yeh KW* (2014) Transplastomic Nicotiana benthamiana plants expressing multiple defense genes encoding protease inhibitors and chitinase display broad spectrum resistance against insects, pathogens, and abiotic stresses. Plant Biotechnol. J. 12:503-515.
  • Chang, DK, Li PC, Lu RM, Jane WN, Kuo YS, Wu HC* (2013) Peptide-mediated liposomal doxorubicin enhances drug delivery efficiency and therapeutic efficacy in animal models. PLOS ONE 8:e83239.
  • Huang, CY, Chen PY, Huang MD, Tsou CH, Jane WN, Huang Anthony HC* ( 2013) Tandem oleosin genes in a cluster acquired in Brassica- ceae created tapetosomes and conferred additive benefit of pollen vigor. PNAS 110:14480-14485.
  • Chang, FP, Kuang LY, Huang CA, Jane WN, Hung Y, Hsing CYI, Mou CY* (2013) A simple plant gene delivery system using mesoporous silica nanoparticles as carriers. J. Mater. Chem. B 1:5279-5287.
  • Su, LW, Chang SH, Li MY, Huang HY, Jane WN, Yang JY* (2013) Purification and biochemical characterization of Arabidopsis At-NEET, an ancient iron-sulfur protein, reveals a conserved cleavage motif forsubcellular localization. Plant Science 213:46-54.