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鄭萬興 (Cheng, Wan-Hsing)

副研究員

  • Ph.D. Plant Molecular and Cellular Biology Program University of Florida
  • M.S. Horticulture National Taiwan University
  • B.S. Horticulture National Chung-Hsing University
  • +886-2-2787-1065(Lab)
  • +886-2-2787-1177(Office)
  • whcheng@gate.sinica.edu.tw
  • 植物荷爾蒙離層酸、生物逆境、和它們的反應

研究主題:植物荷爾蒙離層酸、生物逆境、和它們的反應

植物荷爾蒙離層酸(abscisic acid, ABA)擔任重要角色在調控種子發育、休眠、氣孔關閉、和逆境抗性(圖A)。此外,ABA-調控百合異形葉型(heterophylly) 的產生,此與差異性表現ABA生合成基因NCED3 有關。甚者,離層酸vs. 乙烯(ethylene) 或離層酸vs. 吉貝酸(GA) 具有相互拮抗功能而影響種子發芽,幼苗生長和葉片發育;兩者相互調控對方生合成、代謝、或信息傳遞,進而增強彼此的相互拮抗。

在非生物逆境的研究方面,我們用正向遺傳的方法,播種含T-DNA插入的阿拉伯芥種子於高鹽下,篩選出10個鹽類過度敏感株(salt hypersensitive mutant, sahy)(圖B), 其中,sahy1sahy9具有很強的鹽類過度敏感、植株小、短根、和葉型態等生長缺陷。這兩個蛋白質主要表現於核仁(nucleolus),它們參與核醣體組成(ribosomal biogenesis/assembly)之功能;可能由改變核醣體內蛋白的組成,而造成對特定蛋白質轉譯的效率有所差異。sahy1sahy9顯現出對植物生長激素(auxin)所調控植物生長發育產生缺陷,如突變株的葉脈、葉肉細胞的組織結構、和對植物生長激素(auxin)的反應,皆與正常株不同。而對鹽類過度敏感的機制,部分出自ABA依賴型(ABA-dependent)、ABA非依賴型、和鹽類反應相關基因表現有關。

雖然離層酸生合成和它的receptor基因大部分已被選殖和定性,但是ABA和逆境信息傳遞間之交互反應,仍然被了解不多。為了更進一步了解ABA和逆境信息傳遞間之交互反應,我們利用遺傳的方法,篩選出幾個aba2 suppressor(abs) 突變株(圖C)。這些abs對葡萄糖或鹽類之感應度與正常株相同;不過,若生長在土壤上,這些abs性狀則與aba2和正常株不同,但它們皆缺乏ABA含量。相信此研究對於ABA和養份或鹽類逆境之交互反應機制,會有重大之新發現。

All publication list

Selected publication list

  • Chiang MH, Shen HL, Cheng WH* (2015) Genetic analyses of the interaction between abscisic acid and gibberellins in the control of leaf development in Arabidopsis thaliana. Plant Sci. 236:260-271.
  • Chen YH, Shen HL, Hsu PJ, Hwang SG, Cheng WH* (2014) N-acetylglucosamine-1-P uridylytransferase 1 and 2 are required for gametogenesis and embryo development in Arabidopsis thaliana. Plant Cell Physiol. 55:1977-1993.
  • Hsu YF, Chen YC, Hsiao YC, Wang BJ, Lin SY, Cheng WH, Jauh GY, Harada JJ, Wang CS* (2014) AtRH57, a DEAD RNA helicase, is involved in feedback inhibition of   glucose-mediated abscisic acid accumulation during seedling development and additively affects pre-ribosomal RNA processing with high glucose. Plant J. 77:119-135.
  • Hwang SG, Lin NC, Hsiao YY, Kuo CH, Chang PF, Deng WL, Chiang MH, Shen HL, Chen CY, Cheng WH* (2012) The Arabidopsis short-chain dehydrogenase/reductase 3, an ABSCISIC ACID DEFFICIENT 2 homolog, is involved in plant defense responses but not in ABA biosynthesis. Plant Physiol. Biochem. 51:63-73.
  • Chen HC, Hwang SG, Chen SM, Shii CT, Cheng WH* (2011) ABA-mediated heterophylly is regulated by differential expression of 9-cis-expoxycarotenoid dioxygenase 3 in lilies. Plant Cell Physiol. 52:1806-1821.

研究助理 Research Assistant

沈惠齡 Hwei-Ling Shen

古金格 Grimer A. Perez

李佳芸 Chia-Yun Lee

博士班研究生 Doctoral Student

陳雅惠 Ya-Huei Chen

黃楷超 Kai-Chau Huang

林威至 Wei-Chih Lin