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  4. 林納生

林納生 (Lin, Na-Sheng)

兼任研究員

  • 美國內不拉斯加大學博士
  • 病毒、衛星核酸與宿主植物間之交互作用
  • nslin@sinica.edu.tw
  • +886-2-2787-1022 (Lab: R125)
  • +886-2-2787-1128 (Office: R513)
  • Academia Sinica Archive

I. 病毒與宿主間之分子交互作用

病毒為宿主細胞的絕對寄生物，會挾持受感染之宿主細胞的生理活動迫使其致力於病毒的複製或移動以完成其生活史。然而，植物亦會激發植物賀爾蒙傳導路徑來活化抗病毒基因表現，並利用基因靜默機制來抵禦病毒的感染。此外，當兩種以上不同病毒感染同一植物，常會引起協力作用而加速病毒的複製，是故病毒與植物宿主間之攻防戰存在複雜且緊密之交互關係。我們以感染竹類之竹嵌紋病毒(BaMV)和蘭花最常見之兩種病毒─東亞蘭嵌紋病毒(CymMV)及齒舌蘭輪斑病毒(ORSV)─為研究材料。結合次世代定序分析、遺傳、生化、分生及細胞生物學等方法探討BaMV如何挾持宿主因子或內生性微小RNA，參與其複製及移動；也探討植物離層酸如何活化基因靜默重要因子ARGONAUTE蛋白而抑制病毒之累積。我們以蘭花全方位轉錄體解析CymMV和ORSV之病毒協力作用與蝴蝶蘭的免疫抗性之分子機制，並開發具病毒抗性之轉殖植物。

II. 病毒、衛星核酸與宿主之交互作用

病毒雖為宿主寄生物，然有些病毒會攜帶次病毒分子，例如衛星核酸(satellite RNA)。它與協助病毒雖無序列同源性，但演化出相似之二級RNA結構，以仰賴病毒進行複製及包被。有趣的是，部分干擾型衛星核酸會抑制協助病毒之複製及病徵表現。過去，我們篩選出干擾型之竹嵌紋病毒衛星核酸(satBaMV)，並定位出其抑制BaMV複製之主要決定因子及其分子機制。長期目標上我們想了解衛星核酸是否利用與病毒相同或不同之宿主因子以完成其生活史。近年來我們發現satBaMV藉由fibrillarin核仁蛋白之幫助可在植物體中移動，但fibrillarin並不為病毒移動所需。因此，我們的研究將可提供病毒、衛星核酸與宿主三者交互作用分子機制更深入的探討。

(A) BaMV atomic model showing N- and C-terminal extensions that allow flexibility of the virions, and left-handed helical with ~8.8 subunits per turn.
(B) Synergistic mixed infection of CymMV and ORSV enhanced symptom formation in Phalaenopsis.
(C) Our research interests focus on trilateral interaction among virus, satellite RNA, and host.

All publications

Selected Publications

  • Lin, K. Y., Hsu, F. C., Wu, S. Y., Hsu, Y. H., Lin, N.S.* 2022. MiR398-regulated antioxidants contribute to Bamboo mosaic virus accumulation and symptom manifestation. Plant Physiology 188(1): 593-607.
  • Lee, S. C., Pai, H., He, M. H., Huang, Y. W., Song, Y. L., Kuo, S. Y., Chang, W. C., Hsu, Y. H., Lin, N. S.* 2021. Exploring the Multifunctional Roles of Odontoglossum Ringspot Virus P126 in Facilitating Cymbidium Mosaic Virus Cell-to-Cell Movement during Mixed Infection. Viruses 13(8): 1552-1567.
  • Alazem, M., He, M. H., Chang, C. H., Cheng, N., Lin, N.S.* 2021. Disrupting the Homeostasis of High Mobility Group Protein Promotes the Systemic Movement of Bamboo mosaic virus. Frontiers in Plant Science 11: 597665-597680
  • Alazem, M., Lin, N. S.* 2020. Interplay between ABA signaling and RNA silencing in plant viral resistance. Current Opinion in Virology 42: 1-7.
  • Pai, H., Jean, W. H., Lee, Y. S., Chang, Y. A., Lin, N. S.* 2020. Genome-wide analysis of small RNAs from Odontoglossum ringspot virus and Cymbidium mosaic virus synergistically infecting Phalaenopsis. Molecular Plant Pathology 21(2): 188-205.
  • Chen, T. Y., Pai, H., Hou, L. Y., Lee, S. C., Lin, T. T., Chang, C. H., Hsu, F. C., Hsu, Y. H., Lin, N. S.* 2019. Dual resistance of transgenic plants against Cymbidium mosaic virus and Odontoglossum ringspot virus. Scientific Reports 9(1):10230-10241.
  • Alazem, M., Kim, K. H.*, Lin, N. S.* 2019. Effects of Abscisic Acid and Salicylic Acid on Gene Expression in the Antiviral RNA Silencing Pathway in Arabidopsis. International Journal of Molecular Sciences 20(10): 2538-2553.
  • Hsu, Y. H.*, Tsai CH.*, Lin, N. S.* 2018. Editorial: Molecular Biology of Bamboo mosaic Virus-A Type Member of the Potexvirus Genus. Frontiers in Microbiology 9: 6-7.
  • Tseng K. C., Chiang-Hsieh, Y. F., Pai, H., Chow C. N., Lee S. C., Zheng H. Q., Kuo, P. L., Li, G. Z., Hung, Y. C., Lin N. S.* and Chang W. C.* 2018. MicroRPM: An effective model for novel microRNA prediction based only on small RNA sequencing data without reference genome. Bioinformatics 34:1108-1115.
  • Alazem, M, Lin, N. S.* 2017. Antiviral Roles of Abscisic Acid in Plants. Frontiers in Plant Science 8:1760.
  • . Alazem, M, He, M. H., Moffett, P., Lin, N. S.* 2017. Abscisic acid induces resistance against Bamboo mosaic virus through Argonaute 2 and 3. Plant Physiology 174: 339–355.
  • Lin, K. Y., Lin, N.S*. Interfering Satellite RNAs of Bamboo mosaic virus. Frontiers in Microbiology. 8(787): 1-9.
  • Wang, I. N., Yeh, W. B., Lin, N. S*. 2017. Phylogeography and Coevolution of Bamboo Mosaic Virus and Its Associated Satellite RNA. Frontiers in Microbiology. 8(886): 1-12.
  • Chang, C. H., Hsu, F. C., Lee, S. C., Lo, Y. S., Wang, J. D., Shaw, J., Taliansky, M., Chang, B. Y., Hsu, Y. H., Lin, N. S.* 2016. The nucleolar fibrillarin protein is required for helper virus-independent long-distance trafficking of a subviral satellite RNA in plants. Plant Cell 28(10): 2586-2602.
  • Cheng, N., Lo, Y. S., Ansari, M. l., Ho, K. C., Jeng, S. T., Lin, N. S.*, Dai, H.* 2016. Correlation between mtDNA complexity and mtDNA replication mode in developing cotyledon mitochondria during mung bean seed germination. New Phytologist 213 (2):751-763.
  • DiMaio, F., Chen, C. C., Yu, X., Frenz, B., Hsu, Y. H.*, Lin, N. S.*, Egelman, E. H.* 2015. The molecular basis for flexibility in the flexible filamentous plant viruses. Nature Structural & Molecular Biology 22:642-644.
  • Alazem, M., and Lin, N. S.* 2015. Roles of plant hormones in the regulation of host-virus interactions. Molecular Plant Pathology 16:529-540.
  • Wang, I. N., Hu, C. C., Lee, C. W., Yen, S. M., Yeh, W. B., Hsu, Y. H., and Lin, N. S.* 2014. Genetic Diversity and Evolution of Satellite RNAs Associated with the Bamboo Mosaic Virus. PLoS One 9(9): e108015.
  • Liou, M. R., Huang, Y. W., Hu, C. C., Lin, N. S.*, and Hsu, Y. Y.* 2014. A dual gene-silencing vector system for monocot and dicot plants. Plant Biotechnology Journal 12(3):330-43.
  • Alazem, M., Lin, K. Y., and Lin, N. S.* 2014. The abscisic acid pathway has multifaceted effects on the accumulation of Bamboo mosaic virus. Molecular Plant-Microbe Interaction 27(2): 177-89.
  • Lin, K. Y., Hsu, Y. Y., Chen, H. C. and Lin, N. S.* 2013. Transgenic resistance to Bamboo mosaic virus by expressing interfering satellite RNA. Molecular Plant Pathology 14: 693-707.
  • Chen, H. C., Kong, L. R., Yeh, T. Y., Cheng, C. P., Hsu, Y. H. and Lin, N. S.* 2012. The conserved 5' apical hairpin stem loops of bamboo mosaic virus and its satellite RNA contribute to replication competence. Nucleic Acids Research 40(10): 4641-4652.
  • Vijayapalani, P., Chen, C. F., Liou, M. R., Hsu, Y. H. and Lin, N. S.* 2012. Phosphorylation of Bamboo mosaic virus satellite RNA (satBaMV)-encoded protein P20 downregulates the formation of the satBaMV-P20 ribonucleoprotein complex. Nucleic Acids Research. 40(2): 638-649.
 李淑娟 Chuan-Shu Lee
博士後研究員

林冠妤 Kuan-Yu Lin
博士後研究員

王俊達 Jun-da Wang
博士後研究員

侯良諭 Liang-Yu Hou
博士後研究員

陳婷玉 Ting-yu Chen
研究助理

廖麗施 Alice Liao
研究助理

洪爾慈 Ei-Tsi Hung
研究助理

郭芃妤Peng-Yu Kuo
研究助理

國際

  • 2005 美國科學促進學會會士 - 美國科學促進學會
  • 2001 亞太分子生物學會國際會員 - 亞太分子生物學會

國內

  • 2023 終身成就獎 - 臺灣植物學會
  • 2019 深耕計畫 - 中央研究院
  • 2014 深耕計畫 - 中央研究院
  • 2008 傑出特約研究獎 - 科技部
  • 2006 深耕計畫 - 中央研究院
  • 2005 特約研究獎 - 科技部
  • 2003 教育部學術獎 - 教育部
  • 2003 特約研究獎 - 科技部
  • 2001 尖端研究獎 - 科技部