IPMB | 中央研究院植物暨微生物學研究所

Lin, Na-Sheng (林納生)

Adjunct Research Fellow

Ph.D., Department of Plant Pathology, University of Nebraska -Lincoln, USA (1984)
+886-2-2787-1022(Lab)
+886-2-2787-1128(Office)
nslin@sinica.edu.tw
Molecular interactions of plant viruses, satellite RNA and plants

I. Molecular interactions between viruses and host plants

Viruses are obligate parasites that hijack the host cell’s machinery to complete their life cycles after infection. In contrast, plant hormones regulate the defense response, together with gene silencing, to combat virus invasion. In addition, mixed infection of viruses often results in viral synergism. Thus, comparing the defense and counter-defense interaction between viruses and plants is important. We use Bamboo mosaic potexvirus (BaMV) and the two prevalent orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV), as working materials. By integrating next-generation sequencing, genetics, biochemistry, molecular biology, and cellular biology, we aim to identify host factors and microRNAs involved in BaMV replication and movement, analyze the interaction of abscisic acid-induced resistance with gene silencing, and decipher the molecular mechanisms of viral synergism and anti-viral response in Phalaenopsis co-infected with CymMV and ORSV to generate virus-resistant transgenic plants.

II. Trilateral interactions among viruses, satellite RNAs and host plants

Satellite RNAs (satRNAs) are subviral parasites that are almost exclusively associated with plant viruses. Without appreciable sequence homology, satRNAs evolve similar RNA secondary structures to be dependent on their cognate helper viruses for replication and encapsidation. Some satRNAs can interfere in virus replication and virus-induced symptoms. We identified an satRNA (satBaMV) interfering in a BaMV isolate and mapped its key determinants to downregulate BaMV replication. For our long-term goal, we are interested in analyzing whether satBaMV and BaMV share unique or common host factors to complete their life cycles. Recently, we revealed the differential requirement of fibrillarin, a nucleolar protein, for systemic trafficking of satBaMV and BaMV. Our study will provide mechanistic insight into the replication and movement of satRNAs during virus–satRNA–plant interactions.

(A) BaMV atomic model showing N- and C-terminal extensions that allow flexibility of the virions, and left-handed helical with ~8.8 subunits per turn.
(B) Synergistic mixed infection of CymMV and ORSV enhanced symptom formation in Phalaenopsis.
(C) Our research interests focus on trilateral interaction among virus, satellite RNA, and host.

All publication list

Selected publication list

Lin, K. Y., Hsu, F. C., Wu, S. Y., Hsu, Y. H., Lin, N.S.* 2022. MiR398-regulated antioxidants contribute to Bamboo mosaic virus accumulation and symptom manifestation. Plant Physiology 188(1): 593-607.
Lee, S. C., Pai, H., He, M. H., Huang, Y. W., Song, Y. L., Kuo, S. Y., Chang, W. C., Hsu, Y. H., Lin, N. S.* 2021. Exploring the Multifunctional Roles of Odontoglossum Ringspot Virus P126 in Facilitating Cymbidium Mosaic Virus Cell-to-Cell Movement during Mixed Infection. Viruses 13(8): 1552-1567.
Alazem, M., He, M. H., Chang, C. H., Cheng, N., Lin, N.S.* 2021. Disrupting the Homeostasis of High Mobility Group Protein Promotes the Systemic Movement of Bamboo mosaic virus. Frontiers in Plant Science 11: 597665-597680
Alazem, M., Lin, N. S.* 2020. Interplay between ABA signaling and RNA silencing in plant viral resistance. Current Opinion in Virology 42: 1-7.
Pai, H., Jean, W. H., Lee, Y. S., Chang, Y. A., Lin, N. S.* 2020. Genome-wide analysis of small RNAs from Odontoglossum ringspot virus and Cymbidium mosaic virus synergistically infecting Phalaenopsis. Molecular Plant Pathology 21(2): 188-205.
Chen, T. Y., Pai, H., Hou, L. Y., Lee, S. C., Lin, T. T., Chang, C. H., Hsu, F. C., Hsu, Y. H., Lin, N. S.* 2019. Dual resistance of transgenic plants against Cymbidium mosaic virus and Odontoglossum ringspot virus. Scientific Reports 9(1):10230-10241.
Alazem, M., Kim, K. H.*, Lin, N. S.* 2019. Effects of Abscisic Acid and Salicylic Acid on Gene Expression in the Antiviral RNA Silencing Pathway in Arabidopsis. International Journal of Molecular Sciences 20(10): 2538-2553.
Hsu, Y. H.*, Tsai CH.*, Lin, N. S.* 2018. Editorial: Molecular Biology of Bamboo mosaic Virus-A Type Member of the Potexvirus Genus. Frontiers in Microbiology 9: 6-7.
Tseng K. C., Chiang-Hsieh, Y. F., Pai, H., Chow C. N., Lee S. C., Zheng H. Q., Kuo, P. L., Li, G. Z., Hung, Y. C., Lin N. S.* and Chang W. C.* 2018. MicroRPM: An effective model for novel microRNA prediction based only on small RNA sequencing data without reference genome. Bioinformatics 34:1108-1115.
Alazem, M, Lin, N. S.* 2017. Antiviral Roles of Abscisic Acid in Plants. Frontiers in Plant Science 8:1760.
. Alazem, M, He, M. H., Moffett, P., Lin, N. S.* 2017. Abscisic acid induces resistance against Bamboo mosaic virus through Argonaute 2 and 3. Plant Physiology 174: 339–355.
Lin, K. Y., Lin, N.S*. Interfering Satellite RNAs of Bamboo mosaic virus. Frontiers in Microbiology. 8(787): 1-9.
Wang, I. N., Yeh, W. B., Lin, N. S*. 2017. Phylogeography and Coevolution of Bamboo Mosaic Virus and Its Associated Satellite RNA. Frontiers in Microbiology. 8(886): 1-12.
Chang, C. H., Hsu, F. C., Lee, S. C., Lo, Y. S., Wang, J. D., Shaw, J., Taliansky, M., Chang, B. Y., Hsu, Y. H., Lin, N. S.* 2016. The nucleolar fibrillarin protein is required for helper virus-independent long-distance trafficking of a subviral satellite RNA in plants. Plant Cell 28(10): 2586-2602.
Cheng, N., Lo, Y. S., Ansari, M. l., Ho, K. C., Jeng, S. T., Lin, N. S.*, Dai, H.* 2016. Correlation between mtDNA complexity and mtDNA replication mode in developing cotyledon mitochondria during mung bean seed germination. New Phytologist 213 (2):751-763.
DiMaio, F., Chen, C. C., Yu, X., Frenz, B., Hsu, Y. H.*, Lin, N. S.*, Egelman, E. H.* 2015. The molecular basis for flexibility in the flexible filamentous plant viruses. Nature Structural & Molecular Biology 22:642-644.
Alazem, M., and Lin, N. S.* 2015. Roles of plant hormones in the regulation of host-virus interactions. Molecular Plant Pathology 16:529-540.
Wang, I. N., Hu, C. C., Lee, C. W., Yen, S. M., Yeh, W. B., Hsu, Y. H., and Lin, N. S.* 2014. Genetic Diversity and Evolution of Satellite RNAs Associated with the Bamboo Mosaic Virus. PLoS One 9(9): e108015.
Liou, M. R., Huang, Y. W., Hu, C. C., Lin, N. S.*, and Hsu, Y. Y.* 2014. A dual gene-silencing vector system for monocot and dicot plants. Plant Biotechnology Journal 12(3):330-43.
Alazem, M., Lin, K. Y., and Lin, N. S.* 2014. The abscisic acid pathway has multifaceted effects on the accumulation of Bamboo mosaic virus. Molecular Plant-Microbe Interaction 27(2): 177-89.
Lin, K. Y., Hsu, Y. Y., Chen, H. C. and Lin, N. S.* 2013. Transgenic resistance to Bamboo mosaic virus by expressing interfering satellite RNA. Molecular Plant Pathology 14: 693-707.
Chen, H. C., Kong, L. R., Yeh, T. Y., Cheng, C. P., Hsu, Y. H. and Lin, N. S.* 2012. The conserved 5' apical hairpin stem loops of bamboo mosaic virus and its satellite RNA contribute to replication competence. Nucleic Acids Research 40(10): 4641-4652.
Vijayapalani, P., Chen, C. F., Liou, M. R., Hsu, Y. H. and Lin, N. S.* 2012. Phosphorylation of Bamboo mosaic virus satellite RNA (satBaMV)-encoded protein P20 downregulates the formation of the satBaMV-P20 ribonucleoprotein complex. Nucleic Acids Research. 40(2): 638-649.