DNA Analysis Division, Genomic Technology Core
Research Assistant
- Mei-Jane Fang
- Ming-Ling Cheng
Contact Information
- 02-27871028 (Office)
- 02-27871029 (Lab)
Location
- Room 208,Institute of Plant and Microbial Biology
We provide routine DNA sequencing (using ABI 3730 DNA Analyzer) and Real-time quantitative PCR (Applied Biosystems QuantStudio 12K and QuantStudio 3 real-time PCR systems). The goal of this division of G-TeC is to provide cost effective solutions for routine DNA sequencing and quantitative PCR, including centralized purchasing of consumables and troubleshooting for quantitative PCR and DNA sequencing. The facility also maintains an eGENE HAD-GT12 Genetic Analyzer for common use.
Samples for routine DNA sequencing can be submitted daily to 11:00 A.M. The typical turnaround time from submission of DNA to availability of sequence is 1 day. Cost per sample is NT 80. PeakTrace: post-run analysis of sequence traces by PeakTrace software can increase useable read length for sequences over 700-800 bp. PeakTrace is typically used for plasmid DNA templates. There is an additional charge of NT 3 per sample for PeakTrace analysis; however, this extra charge is only applied if PeakTrace successfully extends the useable read length. The QuantStudio 12K Flex Real-Time PCR System could complete the HRM experiment.
Scheduling of qPCR instrument usage is available through reservation.
► Sample submission form for DNA sequencing
Instruments
