[Wolfgang Schmidt] An imperfect dogma
POST:
In contrast to the straightforwardness of transcriptional surveys, genome-wide profiles of the translational landscape of plant cells remain technically challenging and are thus less well explored. Protein-coding genes are expressed at a variable degree of efficiency, resulting in pronounced discordance among the regulatory levels that govern gene activity. Ribo-Seq is a tremendously useful tool for estimating translation efficiency, but the data sets available for plants are limited. In this Data Descriptor, Isabel Cristina Vélez-Bermúdez (corrsponding author), Wen-Dar Lin, Shu-Jen Chou, Ai-Ping Chen, and Wolfgang Schmidt compare inventories of expressed and translated RNA populations, generated by mRNA sequencing (RNA-Seq) and ribosome footprinting (Ribo-Seq) from shoots and roots of Arabidopsis seedlings. The data set provides information on the translational fitness of protein-coding mRNAs and aid in obtaining a comprehensive picture of the regulatory levels governing genes activity across the genome.