[Wolfgang Schmidt] Translation caught in the actPOST:
Translation caught in the act
Ribosome profiling (Ribo-seq) is a tool to estimate the ‘translational fitness’ of a given messenger RNA (mRNA) in plants in response to environmental or developmental cues with high-resolution. This technique is based on the isolation of mRNA fragments protected by ribosomes followed by massively parallel sequencing of the footprints and allows the measurement of ribosome density along mRNA transcripts. Together with Shu-Jen Chou and Ai-Ping Chen from the Genomic Technology core lab and Wendar Lin (Bioinformatic core lab), Wolfgang Schmidt’s group has developed a detailed step-by-step Ribo-seq protocol adapted for the reference plant Arabidopsis thaliana (STAR Protocols). The corresponding authors are Isabel Vélez-Bermúdez (technical contact) and Wolfgang Schmidt (lead contact).