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[Wolfgang Schmidt] A Proteomic View on the Chromatin Landscape

Chromatin enrichment for proteomics (ChEP) is a technique that allows for unbiased proteomic profiling of the chromatin landscape using mass spectrometry. While the method has been successfully employed to survey chromatin-associated proteins in various organisms and cell types, ChEP has not been applied to plant materials - yet.

As part of a ‘Plant Proteostasis’ issue of ‘Methods in Molecular Biology’ book series, Isabel Cristina Vélez-Bermúdez and Wolfgang Schmidt provide a detailed ChEP protocol, which has been modified for plants and designated ChEP-P (ChEP in Plants). The protocol includes all necessary steps to perform a label-free quantitative ChEP-P experiment, supporting the identification of more than 3500 proteins in Arabidopsis thaliana.

https://link.springer.com/protocol/10.1007/978-1-0716-2784-6_20