loading

  1. 首頁
  2. 人員
  3. 專任研究人員
  4. 馬麗珊

馬麗珊 (Ma, Lay-Sun)

副研究員

植物-真菌交互作用中的分泌效應蛋白

UMAydis@IPMB

玉米黑穗菌Ustilago maydis

玉米黑穗菌是一種雙型態真菌，會感染玉米地上部分的組織並形成大腫瘤，引發黑穗病。在玉米宿主體外，它以單倍體酵母狀細胞生長。一旦感應到植物宿主的環境線索，它就會轉變為寄生性的菌絲。這一發育過程涉及形成具有感染功能的入侵結構、繁殖、腫瘤形成及最終孢子生成。此過程複雜且不可逆，黑穗病原菌需要具備感知宿主環境的能力，並進行精準調控，以利於其在宿主體內生長。

感知環境信號並抑制宿主質外體免疫反應

感知環境信號的能力使黑穗菌能夠從營養生長轉變為絲狀生長，並將其方向導向宿主的養分來源，通過連續波次分泌大量效應蛋白來迅速應對植物的防禦，從而在宿主內完成其生活週期。

我們的研究焦點：黑穗菌效應蛋白

基於效應蛋白在疾病過程中扮演的重要角色，深入研究其生化功能對於理解真菌的致病機制至關重要。全面分析效應蛋白將有助於揭示真菌的致病性和植物防禦機制，進一步加深我們對植物病原擔子菌相互作用的理解，有助於加強作物病害管理策略。

我們的研究主旨

為了揭示黑穗菌的入侵策略並預防初期感染的發生，我們專注於研究位於玉米與 U. maydis 互動界面的效應蛋白，旨在揭示以下機制：

  • 真菌的感知和適應機制
  • 調節效應蛋白的分泌機制
  • 真菌細胞抑制質外體免疫的機制

All publications

Selected Publications

  • John E, Chau MQ, Hoang CV, Chandrasekhar N, Bhaskar CK, and Ma LS*(2023). Fungal cell wall-associated effectors: Sensing, Integration, Suppression, and Protection. MPMI. Mar;37(3):196-210.
  • Lin YH#, Xu MY#, Hsu CC#, Damei FA, Lee HC, Tsai WL, Hoang CV, Chiang YR, and Ma LS* (2023). Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities. Nat Commun. 14, 5755.
  • Ma LS*, Tsai WL, Kalunke MR, Xu MY, Lin YH, Damei AF, and Lee HC (2023). Maize Antifungal Protein AFP1 Elevates Fungal Chitin Levels by Targeting Chitin Deacetylases and Other Glycoproteins. mBio. Mar 22; e0009323.
  • Hoang VC, Bhaskar CK, and Ma LS* (2021). A Novel Effector Vp1 Promotes Fungal Colonization and Virulence of Ustilago maydis. J. Fungi, 7(8): 589.
  • Ludwig N, Reissmann S, Schipper K, Gonzalez C, Assmann D, Glatter T, Moretti M, Ma LS, Rexer KH, Snetselaar K, and Kahmann R* (2021). A Cell Surface-Exposed Protein Complex with an Essential Virulence Function in Ustilago maydis. Nat. Microbiol. 6: 722–730.
  • Yu M, Wang YC, Huang CJ, Ma LS, and Lai EM* (2021). Agrobacterium tumefaciens Deploys a Versatile Antibacterial Strategy to Increase Its Competitiveness. J. Bacteriol. 203(3): e00490-20.
  • Ma LS, Wang L, Trippel C, Mendoza-Mendoza A, Ullman S, Moretti M, Carsten A, Kahnt J, Reissmann S, Zechmann B, Bange G, and Kahmann R* (2018). The Ustilago maydis repetitive effector Rsp3 blocks the antifungal activity of mannose-binding maize proteins. Nat. Commun. 9(1):1711.
  • Ma LS#, Pellegrin C #, and Kahmann R* (2018). Repeat-containing effectors of filamentous pathogens and symbionts. Curr. Opin. Microbiol. 46:123-130 (#equally contributed).
  • Lanver D, Tollot M, Schweizer G, Lo Presti L, Reissmann S, Ma LS, Schuster M, Tanaka S, Liang L, Ludwig N, and Kahmann R* (2017) Ustilago maydis effectors and their impact on virulence. Nat. Rev. Microbiol. 15:409–421.
  • Bondage D, Lin JS, Ma LS, Kuo CH, and Lai EM* (2016). VgrG C-terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex. Proc. Natl. Acad. Sci. USA 113(27): E3931-40.
  • Ma LS, Hachani A, Lin JS, Filloux A*, and Lai EM* (2014). Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta. Cell Host Microbe 16(1): 94–104.
  • Lin JS, Wu HH, Hsu PH, Ma LS, Pang YY, Tsai MD, and Lai EM* (2014). Fha interaction with phosphothreonine of TssL activates type VI secretion in Agrobacterium tumefaciens. PLoS Pathogen 10(3): e1003991.
  • Lin JS, Ma LS, and Lai EM* (2013). Systematic dissection of the Agrobacterium type VI secretion system reveals machinery and secreted components for subcomplex formation. PLoS One. Jul 5; 8(7): e67647.
  • Ma LS, Narberhaus F, and Lai EM* (2012). IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion. J. Biol Chem. 287(19):15610-15621.
  • Ma LS, Lin JS, and Lai EM* (2009). An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker A motif is required for Type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J. Bacteriol. 191: 4316-4329.