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馬麗珊 (Ma, Lay-Sun)

助研究員

  • 2018- Assistant Research Fellow, IPMB, Academia Sinica, Taiwan
  • 2014 PDF, Max Planck Institute for Terrestrial Microbiology, Germany
  • 2012 PDF, IPMB, Academia Sinica, Taiwan
  • 2011 Ph.D., Academia Sinica and National Chung Hsing University
  • +886-2-2787-1146(Lab)
  • +886-2-2787-1145(Office)
  • laysunma@gate.sinica.edu.tw
  • 植物與真菌交互作用之分子機制

Ustilago maydis-maize pathosystem

玉米瘤黑粉病是由Ustilago maydis對玉米的活體寄生所引起的真菌病害。該病原菌需寄生於玉米植株來完成其有性生殖過程。U. maydis對寄主植物的寄生過程,包括形成致病性雙核菌絲體、附著寄主植物表面、穿透寄主表皮、在寄主體內菌絲增殖、使寄主瘤變和生成厚垣孢子。病原真菌與寄主植物之間的互作主要由真菌分泌的效應蛋白所介導。這些效應蛋白根據發揮作用的位置可以分為兩類,有的效應蛋白存在於植物的質外體發揮作用,稱為質外體效應蛋白,有的效應蛋白需要轉運到植物細胞內發揮作用,稱為胞內效應蛋白。植物病原真菌通過分泌大量的效應蛋白調節侵染過程,抑制植物的防禦反應,並從植物組織獲取營養以在植物體定殖。經生物信息學預測,U. maydis的基因組可能編碼大約467個效應蛋白。其中約300個蛋白缺少已知的功能結構域,氨基酸序列十分新穎,同源蛋白僅發現於少數與U. maydis的親緣關係較近的真菌中。但只有僅少數U. maydis效應蛋白在侵染宿主植物過程中的功能得到闡釋。U. maydis通過分泌兩個質外體效應蛋白Pep1和Pit2分別抑制植物防禦反應中產生的過氧化物酶和半胱氨酸蛋白酶。三個胞內效應蛋白Tin2,Cmu1和See1分別通過誘導花青素產生而抑制植物細胞的木質化,改變植物代謝途徑而抑制植物防禦激素水楊酸的產生和刺激細胞分裂而進一步誘導植物腫瘤組織的形成而增強U. maydis在宿主玉米體內的致病性。到目前為止, 大部分分泌的效應蛋白功能未知。我們旨在解析植物病原真菌效應蛋白的致病機制,加強對植物病菌互作的的認識,對防治植物真菌病害具有重要意義。

硏究主旨

我們的研究著重於了解在植物與真菌相互作用中,真菌如何控制疾病和植物抗病性的潛在機制。藉由玉米瘤黑粉病菌為模型系統及其效應蛋白作為分子探針,我們旨在揭示效應蛋白在疾病進展中的生物學功能,及效應蛋白之靶標在植物免疫中扮演的角色,並從中了解植物與此真菌病原之間的相互關聯性。探索效應蛋白功能將提供對植物-微生物相互作用的見解,以達到利用此知識來尋找用於疾病控制和預防的植物抗性基因。

● 研究效應蛋白如何促進真菌在宿主中定植
● 探索植物如何對微生物入侵者採取的防禦行動

Selected publication list

  • Lay-Sun Ma, Lei Wang, Christine Trippel, Artemio Mendoza-Mendoza, Steffen Ullmann, Marino Moretti, Alexander Carsten, Jörg Kahnt, Stefanie Reissmann, Bernd Zechmann, Gert Bange, and Regine Kahmann. (2018) The Ustilago maydis repetitive effector Rsp3 blocks the antifungal activity of mannose-binding maize proteins. Nat. Commun. 2018 Apr 27;9(1):1711.
  • Lay-Sun Ma*, Clément Pellegrin*, and Regine Kahmann. (2018) Repeat-containing effectors of filamentous pathogens and symbionts. Curr. Opin. Microbiol. https://doi.org/10.1016/j.mib.2018.01.007 (*Equal contribution).
  • Daniel Lanver, Marie Tollot, Gabriel Schweizer, Libera Lo Presti, Stefanie Reissmann, Lay-Sun Ma, Mariana Schuster, Shigeyuki Tanaka, Liang Liang, Nicole Ludwig, and Regine Kahmann. (2017) Ustilago maydis effectors and their impact on virulence. Nature Rev. Microbiol. doi:10.1038/nrmicro.2017.33.
  • Devanand Bondage, Jer-Sheng Lin, Lay-Sun Ma, Chih-Horng Kuo, and Erh-Min Lai. (2016) VgrG C-terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex. Proc Natl Acad Sci USA Jul 5; 113(27): E3931-40.
  • Lay-Sun Ma, Abderrahman Hachani, Jer-Sheng Lin, Alain Filloux, and Erh-Min Lai. (2014) Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta. Cell Host Microbe Jul 9; 16(1): 94–104.
  • Jer-Sheng Lin, Hsin-Hui Wu, Pang-Hung Hsu, Lay-Sun Ma, Yin-Yuin Pang, Ming-Daw Tsai, and Erh-Min Lai. (2014) Fha interaction with phosphothreonine of TssL activates type VI secretion in Agrobacterium tumefaciens. PLoS Pathogen. March 10 (3): e1003991.
  • Jer-Sheng Lin, Lay-Sun Ma, and Erh-Min Lai. (2013) Systematic dissection of the Agrobacterium type VI secretion system reveals machinery and secreted components for subcomplex formation. PLoS One. Jul 5; 8(7): e67647.
  • Lay-Sun Ma, Franz Narberhaus, and Erh-Min Lai. (2012) IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion. J. Biol Chem. 287(19):15610-15621.
  • Lay-Sun Ma, Jer-Sheng Lin, and Erh-Min Lai. (2009) An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker A motif is required for Type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J. Bacteriol, 191, 4316-4329.

Li, Hui-Chun (Lab manager)
ayamama@gate.sinica.sinica.tw

Xu, Meng-Yun(Research technician)
mengyun02@gate.sinica.sinica.tw

Raviraj M. Kalunke (Postdoctoral research fellow)
rkalunke@gmail.com

Chibbhi K B (Ph.D. student, TIGP-MBAS program)
chibbhibhaskar@gmail.com

Cuong Hoang Van (Ph.D. student, TIGP-MBAS program)
hvcuong88@gmail.com