loading

Huang, Nien-Chen (黃念貞)

Research Scientist

  • Ph.D. Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica; Graduate institute of Biotechnology, National Chung-Hsing University
  • +886-2-2787-1041
  • nienchen@gate.sinica.edu.tw

RESEARCH EXPERTISE

RNA biology by use of RNA imaging systems and plant grafting techniques

RNA imaging:

  • in situ hybridization
  • in situ RT-PCR
  • fluorescent-based mRNA live-imaging systems to visualize plant mRNAs

Plant grafting techniques:

  • Arabidopsis inflorescence and non-sterile micro-grafting
  • Homo- or hetero-species grafting

Selected publication list

  • Huang NC, Yu TS (2021) Non-sterile grafting methods for Arabidopsis. Methods Mol Biol. 2200:113-119.
  • Luo KR, Huang NC, Yu TS (2020) In vivo imaging of mobile mRNAs in plants using MS2. Methods Mol Biol. 2166:145-155.
  • Huang NC, Luo KR, Yu TS (2018) Mobility of antiflorigen and PEBP mRNAs in tomato-tobacco heterografts. Plant Physiol. 178(2):783-794.
  • Luo KR, Huang NC, Yu TS (2018) Selective targeting of mobile mRNAs to plasmodesmata fro cell-to-cell movement. Plant Physiol. 177(2):604-614.
  • Huang NC, Yu TS (2015) A pin-fasten grafting method provides a non-sterile and highly efficient method for grafting Arabidopsis at diverse developmental stages.Plant Methods 11:38.
  • Huang NC, Jane WN, Chen J, Yu TS (2012) Arabidopsis thaliana CENTRORADIALIS homologues (ATC) acts systemically to inhibit floral initiation in Arabidopsis. Plant J. 72(2):175-84.
  • Lu KJ, Huang NC, Liu YS, Lu CA, Yu TS (2012) Long-distance movement of Arabidopsis FLOWERING LOCUS T RNA participates in systemic floral regulation. RNA Biol. 9(5):653-62.