Ho, Chin-Min (何金敏)
Assistant Research Fellow
- 2018.Jan – 2018.Jun PDF Department of Microbiology and immunology, Stanford University
- 2012-2017 PDF Department of Biology, Stanford University
- 2005-2011 PhD Biochemistry and Molecular Biology
- 1999-2003 BS Department of Botany, National Taiwan University
- Inter-organelle communication and cell division
- Lab Website
Inter-organelle communication and its related signaling
Organelles have been treated as individual compartments with defined composition and organization in a cell. However, there is emerging evidence showing that inter-organelle communication through membrane contact sites is important for cellular functions and organismal homeostasis.
By using forward genetic screen, I have found a link between ER-PM (endoplasmic reticulum-plasma membrane) contacts and the receptor like kinase (RLK)-mediated developmental signaling. VST proteins play a role in ER-PM tethering. vst mutants exhibit stomatal phenotype and have less lipid droplets compared to wild type plants. In my lab, we will use the VSTs as an anchor to identify protein and lipid components of the tethering sites. The goal is to understand the function/organization of membrane contact sites and inter-organelle communication in the context of development and physiology in plants.
Cellular machinery involved in symmetric and asymmetric cell divisions using stomatal development as a model
A stomatal lineage is initiated from asymmetric cell division to produce two sister cells. The small cell (red) undergoes symmetric division to form a stoma.
The stomatal lineage in Arabidopsis uses both asymmetric and symmetric cell divisions to produce a pair of guard cells. What are the players in cell division during stomatal development? Do plants use the same cellular machinery for cell divisions or specific machineries for asymmetric or symmetric division? By combining FACS-sorting, transcriptome analysis and comparison with other stomatal lineage cells, we will identify players involved in these transitions. In addition, we will further investigate the function and specificity of these cellular components during symmetric and asymmetric cell divisions in stomatal development.
Selected publication list
- Ho, C.-M.K., Paciorek, T., Abrash, E., and Bergmann, D.C. (2016). Modulators of stomatal lineage signal transduction alter membrane contact sites and reveal specialization among ERECTA Kinases. Dev. Cell 38, 345–357.
- Hotta, T., Kong, Z., Ho, C.M.K., Zeng, C.J.T., Horio, T., Fong, S., Vuong, T., Lee, Y.R.J., and Liu, B. (2012). Characterization of the Arabidopsis augmin complex uncovers its critical function in the assembly of the acentrosomal spindle and phragmoplast microtubule arrays. The Plant Cell 24:1494-1509
- Ho, C.M.K., Kiyama, L., Liu, B. (2012). The Arabidopsis MAP65-3 Protein Cross-links Anti-Parallel Microtubules toward Their Plus Ends in the Phragmoplast via Its Distinct C-termianl Microtubule-binding Domain. The Plant Cell. 24, 2071-2085.
- Ho, C.M.K., Hotta, T., Guo, F., Roberson, R.W., Lee, Y.R., and Liu, B. (2011). Interaction of anti-parallel microtubules in the phragmoplast is mediated by the microtubule-associated protein MAP65-3 in Arabidopsis. The Plant Cell. 23, 2909-2923.
- Ho, C.M.*, Hotta, T.*, Kong, Z.*, Zeng, C.J.*, Sun, J., Lee, Y.R., and Liu, B. (2011). Augmin Plays a Critical Role in Organizing the Spindle and Phragmoplast Microtubule Arrays in Arabidopsis. The Plant Cell. 23, 2606-2618 (* Equal contribution).