[Wolfgang Schmidt] All Together Now!

Co-immunoprecipitation (Co-IP) is a widely used and powerful approach for studying protein-protein interactions in vivo. In a collaboration with Marta Riera and David Caparros-Ruiz from the Centre de Recerca en Agrigenòmica (CRAC) in Barcelona (Spain) initiated by Isabel Vélez-Bermúdez from Wolfgang Schmidt’s lab, a state-of-the-art guide for antibody purification and immobilization followed by immunoprecipitation from plant tissue extracts using magnetic beads has recently been published in the journal STAR Protocols. The protocol has been previously used to detect regulators in the Zea mays phenylpropanoid pathway and is amenable to a variety of downstream assays, including western blot and mass spectrometry.