Full publication list in google scholar
2024
Announcement
Complete genome sequence of Sphingomonas sp. strain R1, a bacterium isolated from tomato (Solanum lycopersicum)
Yen HC, Wang SC, Chen WM, Lai EM*, Kuo CH*.
Article
Agrobacteria deploy two classes of His-Me finger superfamily nuclease effectors exerting different antibacterial capacities against specific bacterial competitors.
Santos MN, Pintor KL, Hsieh PY, Cheung YW, Sung LK, Shih YL., & Lai EM*.
2023
Protocol
Protein–Protein Interactions: Co-immunoprecipitation.
Lin JS, Ali J, Lai EM*.
Review
Virulence and ecology of Agrobacteria in the context of evolutionary genomics.
Weisberg AJ, Wu Y, Chang JH, Lai EM, Kuo CH*.
Article
A glycine zipper motif is required for the translocation of a T6SS toxic effector into target cells.
Ali J, Yu M, Sung LK, Cheung YW, Lai EM*.
Article
Soil Inoculation and Blocker-Mediated Sequencing Show Effects of the Antibacterial T6SS on Agrobacterial Tumorigenesis and Gallobiome.
Wang SC, Chen AP, Chou SJ, Kuo CH, Lai EM*.
Protocol
In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography.
Lai L, Cheung YW, Martinez M, Kixmoeller K, Palao L 3rd, Steimle S, Ho MC, Black BE, Lai EM*, Chang YW.
2022
Idea Revisted
Distinct TssA proteins converge in coordinating tail biogenesis of the type VI secretion systems.
Ali J and Lai EM*.
Protocol
AGROBEST: a highly efficient Agrobacterium-mediated transient expression system in Arabidopsis seedlings.
Wu HY and Lai EM*.
Methods Mol Biol Plant Synthetic Biology: Methods and Protocols. 2022.
2020
Review
Role of recipient susceptibility factors during contact-dependent interbacterial competition.
Lin HH, Filloux A and Lai EM*.
Article
Agrobacterium tumefaciens deploys a versatile antibacterial strategy to increase its competitiveness.
Yu M, Wang YC, Huang CJ, Ma LS, and Lai EM*.
2019
Article
A high-throughput interbacterial competition screen identifies ClpAP in enhancing recipient susceptibility to type VI secretion system-mediated attack by Agrobacterium tumefaciens.
Lin HH, Yu M, Sriramoju MK, Hsu ST, Liu CT*, and Lai EM*.
Article
Redundancy and specificity of type VI secretion vgrG loci in antibacterial activity of Agrobacterium tumefaciens 1D1609 strain.
Santos MNM, Cho ST, Wu CF, Chang CJ, Kuo CH, and Lai EM*.
Article
Effector loading onto the VgrG carrier activates type VI secretion system assembly.
Wu CF, Lien YW, Bondage D, Lin JS, Pilhofer M, Shih YL, Chang JH, Lai EM*.
Article
Plant pathogenic Agrobacterium tumefaciens strains have diverse type VI effector-immunity pairs and vary in in planta competitiveness.
Wu CF, Santos Santos MNM, Cho ST, Chang HH, Tsai YM, Smith DA, Kuo CH*, Chang JH*, Lai EM*.
2018
Article
Stable pH suppresses defense signaling and is the Key to enhance Agrobacterium-mediated transient expression in Arabidopsis seedlings.
Wang YC, Yu M, Shih PY, Wu HY, Lai EM*.
Article
TagF-mediated repression of bacterial type VI secretion systems involves a direct interaction with the cytoplasmic protein Fha.
Lin JS, P Panayiota, Wu HH, Tsai MD, Filloux A, and Lai EM*.
Article
Differential roles of glucosinolates and camalexin at different stages of Agrobacterium-Mediated Transformation.
Shih PY, Chou SJ, Mueller C, Halkier BA, Deeken R, and Lai EM*.
2017
Review
Agrobacterium-mediated plant transformation: biology and applications.
Hwang HH*, Yu M, and Lai EM*.
Review
Type VI secretion effectors: methodologies and biology.
Lien YW and Lai EM*.
Preview
Warfare between host immunity and bacterial weapons.
Yu M and Lai EM*.
2016
Article
VgrG C-terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex.
Bondage D, Lin JS, Ma LS, Kuo CH, and Lai EM*.
2014
Article
Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta.
Ma LS, Hachani A, Lin JS, Filloux A*, and Lai EM*.
Article
Expression and functional characterization of the Agrobacterium VirB2 amino acid substitution variants in T-pilus biogenesis, virulence, and transient transformation efficiency.
Wu HY, Chen CY and Lai EM*.
Article
AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analysis in Arabidopsis seedlings.
Wu HY, Liu KH, Wang YC, Wu JF, Chiu WL, Chen CY, Wu SH, Sheen J, and Lai EM*.
Article
Fha interaction with phosphothreonine of TssL activates type VI secretion in Agrobacterium tumefaciens.
Lin JS, Wu HH, Hsu PH, Ma LS, Pang YY, Tsai MD, and Lai EM*.