Aug 31, 2026 5-min Highlight

[Xuan Lai] Programmable design of synthetic plant immune receptors for pathogen protein recognition

[Erh-Min Lai] BackBone Builder (B3): a modular Golden Gate standard with a compatible Agrobacterium parts library

Aug 24, 2026 Journal Club (IPMB-PEM)

[Jou-Chi Li] The RING-Type Ubiquitin Ligase XBAT35.2 Positively Modulates Plant Immunity by Promoting FLS2 Protein Accumulation

Aug 17, 2026 5-min Highlight

[Mao-Sen Liu] AgroGem: A Rapid and Scalable Transient Transformation System for Functional Genetics in Multiple Plant Species

[Jou-Chi Lin] Immune activation by a phloem-colonizing plant pathogen drives citrus Huanglongbing disease development

Aug 3, 2026 5-min Highlight

[Teng-Kuei Huang] Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana

[Hagos M. Juhar] Endogenous expression of the wound signal peptide ZmREF1 enhances Agrobacterium-mediated transformation of maize inbred B104

Jul 27, 2026 5-min Highlight

[Erh-Min Lai] Polθ‐mediated T‐DNA integration is a conserved mechanism across plant species

[Li-Kang Sung] Streptomyces produce a diphtheria toxin-like exotoxin that targets insects

Jun 26, 2026 5-min Highlight

[Xuan Lai] Plant immune dysregulation disrupts microbe-induced growth promotion and microbiome compatibility

[Yi-Chieh Wang] A regulatory network promotes apoplastic alkalinization to prime plant immunity in tissues distal to site of infection

[Yung-Hui Wen] Evolutionary Coupling of Flagellar Motility and Type VI Secretion Systems Across Bacteria

Jun 1, 2026 5-min Highlight

[Mao-Sen Liu] A rapid and efficient plant genetic transformation system based on direct delivery

[Jou-Chi Lin] The RING-Type Ubiquitin Ligase XBAT35.2 Positively Modulates Plant Immunity by Promoting FLS2 Protein Accumulation

May 4, 2026 5-min Highlight

[Hagos M. Juhar] Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid

[Teng-Kuei Huang] Synergistic engineering of Casδ nuclease for robust genome editing

Apr 20, 2026 5-min Highlight

[Yi-Chieh Wang] A new use of Agrobacterium plant growth regulator genes for plant bioengineering

Mar 23, 2026 5-min Highlight

[Xuan Lai] Effector conformational plasticity enables lineage-specific secretion via Hcp heterohexamers in gut symbiont

[Erh-Min Lai] The Ti Plasmid-Encoded VirJ Functions as a Lysyl-Phosphatidylglycerol Hydrolase in Agrobacterium tumefaciens

Mar 9, 2026 5-min Highlight

[Mao-Sen Liu] Improved rapid and efficient hairy root transformation using Rhizobium rhizogenes in legume crops

[Jou-Chi Lin] Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop an improved suite of strains

[Hsi-Ching Yen] Myosin VIII and XI isoforms interact with the Agrobacterium VirE2 protein and facilitate its transport from the plasma membrane to the perinuclear region during plant transformation

Mar 2, 2026 Journal Club (IPMB-PEM)

[Xuan Lai] A bacterial nutrition strategy for plant disease control

Feb 9, 2026 5-min Highlight

[Teng-Kuei Huang] Heritable, Tissue Culture-Independent and Transgene-Free Genome Editing in Plants via Viral Delivery of CRISPR/AsCas12f

[Hagos M. Juhar] Decoding plant defense signaling using the defenseless mutant

Jan 26, 2026 5-min Highlight

[Yi-Chieh Wang] Type IV Secretion System Drives Lipid Mixing

[Li-Kang Sung] Strain Displacement in Microbiomes via Ecological Competition

Jan 12, 2026 5-min Highlight

[Xuan Lai] Interkingdom sensing of fungal tyrosol promotes bacterial antifungal T6SS activity in the murine gut

[Yung-Hui Wen] A small periplasmic protein governs broad physiological adaptations in Vibrio cholerae via regulation of the DbfRS two-component system

[Erh-Min Lai] Binary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdoms

Dec 16, 2025 5-min Highlight

[Yu Wu] Dynamic transcriptome and GWAS uncover a hydroxyproline-rich glycoprotein that suppresses Agrobacterium-mediated transformation in maize

[Mao-Sen Liu] A single-cell, spatial transcriptomic atlas of the Arabidopsis life cycle

[Jou-Chi Lin] Trichome formation in Nicotiana benthamiana is induced by Agrobacterium

Dec 8, 2025 Journal Club (IPMB-PEM)

[Hagos M. Juhar] Chromosome architecture affects virulence and competitiveness in Agrobacterium tumefaciens C58

Dec 1, 2025 5-min Highlight

[Teng-Kuei Huang] SERK5 genetically compensates for the loss of BAK1 and BKK1 in regulating elf18-triggered immune signaling in Arabidopsis

[Hagos M. Juhar] Isolation and characterization of Agrobacterium vacciniicorymbosi sp. nov. originating from crown galls in blueberry (Vaccinium corymbosum) in Serbia

Nov 18, 2025 5-min Highlight

[Li-Kang Sung] Intraspecies warfare restricts strain coexistence in human skin microbiomes

[Yi-Chieh Wang] Extracellular pH sensing by plant cell-surface peptide-receptor complexes

[Hao-Wen Hsu] TssW-PpkA-Fha axis controls the positioning and initiation of the type VI secretion system in Acidovorax citrulli

Oct 21, 2025 5-min Highlight

[Xuan Lai] A ubiquitous mobile genetic element changes the antagonistic weaponry of a human gut symbiont

[Yung-Hui Wen] T6SS4 is heterogeneously expressed in Yersinia pseudotuberculosis and is a target for transcriptional and post-transcriptional regulation

Sep 8, 2025 Journal Club (IPMB-PEM)

[Li-Kang Sung] Functional replacement of ancestral antibacterial secretion system in a bacterial plant pathogen

Jun 23, 2025 5-min Highlight

[Erh-Min Lai] Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology

[Yi-Chieh Wang] The Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Agrobacterium fabrum Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors

[Yung-Hui Wen] Molecular bases for the loss of type VI secretion system activity during enteroaggregative E. coli experimental evolution

Jun 2, 2025 Journal Club (IPMB-PEM)

[Teng-Kuei Huang] Quantitative dissection of Agrobacterium T-DNA expression in single plant cells reveals density-dependent synergy and antagonism

May 26, 2025 5-min Highlight

[Xuan Lai] Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation

[Li-Kang Sung] Pseudomonas syringae subpopulations cooperate by coordinating flagellar and type III secretion spatiotemporal dynamics to facilitate plant infection

May 12, 2025 5-min Highlight

[Teng-Kuei Huang] Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis

[Yu Wu] Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens

Apr 28, 2025 5-min Highlight

[Hagos M. Juhar] Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation

Apr 14, 2025 5-min Highlight

[Erh-Min Lai] A rare PRIMER cell state in plant immunity

Mar 31, 2025 5-min Highlight

[Yi-Chieh Wang] Reversible ubiquitination conferred by domain shuffling controls paired NLR immune receptor complex homeostasis in plant immunity

Mar 17, 2025 5-min Highlight

[Xuan Lai] A WYL domain transcription factor regulates Lactiplantibacillus plantarum intestinal colonization via perceiving c-di-GMP

[Yung-Hui Wen] Distribution of the four type VI secretion systems in Pseudomonas aeruginosa and classification of their core and accessory effectors

Mar 10, 2025 Journal Club (IPMB-PEM)

[Yung-Hui Wen] A necrotizing toxin enables Pseudomonas syringae infection across evolutionarily divergent plants

Mar 3, 2025 5-min Highlight

[Mao-Sen Liu] The OXIDATIVE SIGNAL-INDUCIBLE1 kinase regulates plant immunity by linking microbial pattern–induced reactive oxygen species burst to MAP kinase activation

[Teng-Kuei Huang] Evolution of a vascular plant pathogen is associated with the loss of CRISPR-Cas and an increase in genome plasticity and virulence genes

Feb 17, 2025 5-min Highlight

[Hagos M. Juhar] Agrobacterium-mediated Cuscuta campestris transformation as a tool for understanding plant–plant interactions

[Yu Wu] Generation and Assessment of Soybean (Glycine max (L.) Merr.) Hybrids for High-Efficiency Agrobacterium-Mediated Transformation

Jan 13, 2025 5-min Highlight

[Li-Kang Sung] Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complex

[Erh-Min Lai] Lysozyme-coated nanoparticles for active uptake and delivery of synthetic RNA and plasmid-encoded genes in plants

Dec 30, 2024 5-min Highlight

[Xuan Lai] Acinetobacter baumannii represses type VI secretion system through a manganese-dependent small RNA-mediated regulation

[Mao-Sen Liu] The leucine-rich repeat receptor kinase QSK1 regulates PRR-RBOHD complexes targeted by the bacterial effector HopF2Pto

Dec 16, 2024 5-min Highlight

[Yung-Hui Wen] Mechanisms of P. aeruginosa resistance to Type VI Secretion System attacks

[Yu Wu] Atypical rhizobia trigger nodulation and pathogenesis on the same legume hosts

Dec 9, 2024 Journal Club (IPMB-PEM)

[Teng-Kuei Huang] Binary vector copy number engineering improves Agrobacterium-mediated transformation

Dec 2, 2024 5-min Highlight

[Yi-Chieh Wang] Capsular Polysaccharide Restrains Type VI Secretion in Acinetobacter baumannii

[Li-Kang Sung] Archaeal type six secretion system mediates contact-dependent antagonism

[Hagos M. Juhar] A simplified Agrobacterium tumefaciens–mediated transformation protocol accelerates plant molecular breeding

Sep 23, 2024 5-min Highlight

[Erh-Min Lai] WHIX is a T6SS secretion domain found in polymorphic double-edged sword effectors

[Teng-Kuei Huang] uantification of evolved DNA-editing enzymes at scale with DEQSeq

Sep 16, 2024 Journal Club (IPMB-PEM)

[Mao-Sen Liu] Enhancing Agrobacterium-mediated Plant Transformation Efficiency through Improved Ternary Vector Systems and Auxotrophic Strains

Aug 26, 2024 5-min Highlight

[Xuan Lai] A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri

[Yung-Hui Wen] A whole-cell platform for discovering synthetic cell adhesion molecules in bacteria

Jul 22, 2024 Journal Club (IPMB-PEM)

[Xuan Lai] Volatile methyl jasmonate from roots triggers host-beneficial soil microbiome biofilms

Jul 15, 2024 5-min Highlight

[Yi-Chieh Wang] OsSRF8 interacts with OsINP1 and OsDAF1 to regulate pollen aperture formation in rice

[Yu Wu] A dual RNA-seq analyses revealed dynamic arms race during the invasion of walnut by Colletotrichum gloeosporioides

May 20, 2024 5-min Highlight

[Yung-Hui Wen] Constitutive expression of the Type VI Secretion System carries no measurable fitness cost in Vibrio cholerae

[Li-Kang Sung] Generalized biomolecular modeling and design with RoseTTAFold All-Atom; Accurate structure prediction of biomolecular interactions with AlphaFold 3

[Erh-Min Lai] Streptomyces umbrella toxin particles block hyphal growth of competing species

[Sin-Sin Hsu] Type 1 fimbriae-mediated collective protection against type 6 secretion system attacks

May 6, 2024 5-min Highlight

[Xuan Lai] PIX is an N-terminal delivery domain that defines a class of polymorphic T6SS effectors in Enterobacterales

Apr 22, 2024 5-min Highlight

[Teng-Kuei Huang] Boosting genome editing in plants with single transcript unit surrogate reporter systems

[Teng-Kuei Huang] Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor root stocks

[Mao-Sen Liu] Molecular analysis of the role of polymerase theta in gene targeting in Arabidopsis thaliana

Apr 8, 2024 5-min Highlight

[Yi-Chieh Wang] Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana

[Yu Wu] Transcriptome profiling of type VI secretion system core gene tssM mutant of Xanthomonas perforans highlights regulators controlling diverse functions ranging from virulence to metabolism

[Hagos M. Juhar] An efficient genetic transformation system mediated by Rhizobium rhizogenes in fruit trees based on the transgenic hairy root to shoot conversion

Mar 25, 2024 5-min Highlight

[Xuan Lai] A PilZ domain protein interacts with the transcriptional regulator HinK to regulate type VI secretion system in Pseudomonas aeruginosa

[Erh-Min Lai] LLPS condensates of Fha initiate the inside-out assembly of the type VI secretion system

[Li-Kang Sung] Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors

Mar 18, 2024 Journal Club (IPMB-PEM)

[Li-Kang Sung] Leaf microbiome dysbiosis triggered by T2SS-dependent enzyme secretion from opportunistic Xanthomonas pathogens

Jan 29, 2024 5-min Highlight

[Yung-Hui Wen] Trojan horse-like T6SS effector TepC mediates both interference competition and exploitative competition

[Sin-Sin Hsu] Flagella are required to coordinately activate competition and host colonization factors in response to a mechanical signal

Jan 15, 2024 5-min Highlight

[Mao-Sen Liu] Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution

[Teng-Kuei Huang] Split complementation of base editors to minimize off-target edits

Dec 25, 2023 5-min Highlight

[Yu Wu] Transcriptome architecture of the three main lineages of agrobacteria

[Si-Chong Wang] Genetically refactored Agrobacterium-mediated transformation

[Yi-Chieh Wang] Green leaf volatile sensory calcium transduction in Arabidopsis

[Hagos M. Juhar] A rapid and highly efficient sorghum transformation strategy using GRF4‐GIF1/ternary vector system

Dec 18, 2023 5-min Highlight

[Erh-Min Lai] The mating pilus of E. coli pED208 acts as a conduit for ssDNA during horizontal gene transfer

[Sin-Sin Hsu] The F pilus serves as a conduit for the DNA during conjugation between physically distant bacteria

[Yung-Hui Wen] No fitness cost entailed by type VI secretion system synthesis, assembly, contraction, or disassembly in enteroaggregative Escherichia coli

Dec 6, 2023 5-min Highlight

[Xuan Lai] c-di-GMP inhibits the DNA binding activity of H-NS in Salmonella

[Li-Kang Sung] Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation

Nov 6, 2023 5-min Highlight

[Yi-Hsuan Hou] NLR immune receptor–nanobody fusions confer plant disease resistance

[Mao-Sen Liu] Blueprint for non-transgenic edited plants

[Teng-Kuei Huang] Deletions within intronic T-DNA lead to reversion of T-DNA mutant phenotypes

Oct 30, 2023 Journal Club (IPMB-PEM)

[Yi-Hsuan Hou] Functional diversification of a wild potato immune receptor at its center of origin

Sep 25, 2023 5-min Highlight

[Yu Wu] Time-Course Transcriptome Analysis of Bacillus subtilis DB104 during Growth

[Hagos M. Juhar] Functional diversification of a wild potato immune receptor at its center of origin

[Yi-Chieh Wang] Qualitative and Quantitative Methods to Measure Antibacterial Activity Resulting from Bacterial Competition

[Si-Chong Wang] Ligand-Displaying E. coli Cells and Minicells for Programmable Delivery of Toxic Payloads via Type IV Secretion Systems

Aug 28, 2023 5-min Highlight

[Xuan Lai] Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae

[Erh-Min Lai] Plant extracellular self-DNA inhibits growth and induces immunity via the jasmonate signaling pathway

[Yee-Wai Cheung] A role for the Gram-negative outer membrane in bacterial shape determination

Aug 14, 2023 5-min Highlight

[Sin-Sin Hsu] Elicitor-induced plant immunity relies on amino acids accumulation to delay the onset of bacterial virulence

Jul 24, 2023 Journal Club (IPMB-PEM)

[Hagos M. Juhar] A modified Agrobacterium-mediated transformation for two oomycete pathogens

Jul 3, 2023 5-min Highlight

[Mao-Sen Liu] Early detection of late blight in potato by whole-plant redox imaging

[Teng-Kuei Huang] An efficient CRISPR–Cas12a promoter editing system for crop improvement

Jun 5, 2023 5-min Highlight

[Yu Wu] Shifting microbial communities can enhance tree tolerance to changing climates

[Hagos M. Juhar] Plant commensal type VII secretion system causes iron leakage from roots to promote colonization

[Yi-Chieh Wang] Collective protection against the type VI secretion system in bacteria

[Si-Chong Wang] Specific modulation of the root immune system by a community of commensal bacteria

May 22, 2023 5-min Highlight

[Yee-Wai Cheung] Mechanism of secretion of TcpF by the Vibrio cholerae toxin-coregulated pilus

[Xuan Lai] Programmable protein delivery with a bacterial contractile injection system

[Li-Kang Sung] The accessory protein TagV is required for full Type VI secretion system activity in Serratia marcescens

[Erh-Min Lai] An E1–E2 fusion protein primes antiviral immune signalling in bacteria

[Erh-Min Lai] Ubiquitin-like conjugation by bacterial cGAS enhances anti-phage defence

May 15, 2023 Journal Club (IPMB-PEM)

[Yung-Hui Wen] Membrane-localized expression, production and assembly of Vibrio parahaemolyticus T3SS2 provides evidence for transertion

May 8, 2023 5-min Highlight

[Jemal Ali] The outer membrane and peptidoglycan layer form a single 2 mechanical device balancing turgor

[Sin-Sin Hsu] The Vibrio fischeri type VI secretion system incurs a fitness cost under host-like conditions

Apr 24, 2023 5-min Highlight

[Yu Wu] Back to the Roots: Agrobacterium-Specific Phages Show Potential to Disinfect Nutrient Solution from Hydroponic Greenhouses

[Hagos M. Juhar] The gene TaWOX5 overcomes genotype dependency in wheat genetic transformation

[Mao-Sen Liu] The Chloroplast Envelope of Angiosperms Contains a Peptidoglycan Layer

[Yi-Chieh Wang] Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture

[Teng-Kuei Huang] Induction of T-DNA amplification by retrotransposon-derived sequences

Apr 10, 2023 5-min Highlight

[Yung-Hui Wen] Paradoxical activation of a type VI secretion system (T6SS) phospholipase effector by its cognate immunity protein

Mar 27, 2023 5-min Highlight

[Erh-Min Lai] Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression

[Xuan Lai] A Library of Promoter-gfp Fusion Reporters for Studying Systematic Expression Pattern of Cyclic-di-GMP Metabolism-Related Genes in Pseudomonas aeruginosa

[Li-Kang Sung] Broader functions of TIR domains in Arabidopsis immunity

[Jie Yang] Imaging of Escherichia coli K5 and glycosaminoglycan precursors via targeted metabolic labeling of capsular polysaccharides in bacteria

[Yee-Wai Cheung] A c-di-GMP Binding Effector Controls Cell Size in a Cyanobacterium

Mar 13, 2023 5-min Highlight

[Si-Chong Wang] Community science designed ribosomes with beneficial phenotypes

[Jemal Ali] Specialized pathogenic cells release Tc toxins using a type 10 secretion system

[Yi-Hsuan Hou] Calcineurin B-like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice

[Sin-Sin Hsu] Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions

Mar 2, 2023 5-min Highlight

[Yi-Chieh Wang] Calcium Promotes T6SS-Mediated Killing and Aggregation between Competing Symbionts

[Yu Wu] Multiplicity of Agrobacterium infection of Nicotiana benthamiana for transient DNA delivery

[Teng-Kuei Hwang] evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library

[Mao-Sen Liou] Nanocarrier spray: a nontransgenic approach for crop engineering

Feb 1, 2023 5-min Highlight

[Yu Wu] Gene flow and introgression are pervasive forces shaping the evolution of bacterial species

[Si-Chong Wang] Contrasting patterns of microbial dominance in the Arabidopsis thaliana phyllosphere

Jan 17, 2023 5-min Highlight

[Erh-Min Lai] Activation of ChvG-ChvI regulon by cell wall stress confers resistance to β-lactam antibiotics and initiates surface spreading in Agrobacterium tumefaciens

[Xuan Lai] Bacterial volatile organic compounds attenuate pathogen virulence via evolutionary trade-offs

[Yee-Wai Cheung] A typical Legionella GTPase effector hijacks host vesicular transport factor p115 to regulate host lipid droplet

Jan 9, 2023 Journal Club (IPMB-PEM)

[Teng-Kuei Hwang] The translocated virulence protein VirD5 causes DNA damage and mutation during Agrobacterium-mediated transformation of yeast

Dec 22, 2022 5-min Highlight

[Yung-Hui Wen] The activated ClpP peptidase forcefully grips a protein substrate

[Sin-Sin Hsu] Cryo-EM structure of the Agrobacterium tumefaciens T-pilus reveals the importance of positive charges in the lumen

[Jemal Ali] A secreted effector with a dual role as a toxin and as a transcriptional factor

Dec 8, 2022 5-min Highlight

[Mao-Sen Liou] Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis

[Teng-Kuei Hwang] The translocated virulence protein VirD5 causes DNA damage and mutation during Agrobacterium-mediated transformation of yeast

[Yi-Chieh Wang] Heterologous Assembly of the Type VI Secretion System Empowers Laboratory Escherichia coli with Antimicrobial and Cell Penetration Capabilities

Nov 28, 2022 Journal Club (IPMB-PEM)

[Yu Wu] Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system

Nov 23, 2022 5-min Highlight

[Si-Chong Wang] PSKR1 balances the plant growth-defense trade-off in the rhizosphere microbiome

[Yu Wu] Xanthomonas Infection Transforms the Apoplast into an Accessible and Habitable Niche for Salmonella enterica

[Yi-Hsuan Hou] A conserved protein disulfide isomerase enhances plant resistance against herbivores

Nov 10, 2022 5-min Highlight

[Xuan Lai] Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system

[Erh-Min Lai] A Comprehensive Phenotypic Screening Strategy to Identify Modulators of Cargo Translocation by the Bacterial Type IVB Secretion System

[Li-Lang Sung] Engineering AraC to make it responsive to light instead of arabinose

[Jennifer A.] Mapping the relative abundance of soil microbiome biodiversity from eDNA and remote sensing

Oct 27, 2022 5-min Highlight

[Yung-Hui Wen] Antibacterial T6SS effectors with a VRR-Nuc domain are structure-specific nucleases

[Sin-Sin Hsu] Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context

Oct 17, 2022 Journal Club (IPMB-PEM)

[Mao-Sen Liou] An inducible potato (E,E)-farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco

Oct 5, 2022 5-min Highlight

[Yi-Chieh Wang] Germline transformation of Artemisia annua L. plant via in planta transformation technology “Floral dip”

[Mao-Sen Liou] Functional characterization of a defense-responsive bulnesol/elemol synthase from potato

[Teng-Kuei Hwang] CRISPR/Cas9-mediated generation of fls2 mutant in Nicotiana benthamiana for investigating the flagellin recognition spectrum of diverse FLS2 receptors

Sep 23, 2022 5-min Highlight

[Sin-Sin Hsu] A binary effector module secreted by a type VI secretion system

[Si-Chong Wang] Trade-offs constrain adaptive pathways to T6 survival

[Yu Wu] Trophic interactions between predatory protists and pathogen-suppressive bacteria impact plant health

Aug 22, 2022 5-min Highlight

[Li-Kang Sung] Prokaryotic innate immunity through pattern recognition of conserved viral proteins

[Erh-Min Lai] Mating pair stabilization mediates bacterial conjugation species specificity

Aug 15, 2022 Journal Club (IPMB-PEM)

[Si-Chong Wang] Mapping phyllosphere microbiota interactions in planta to establish genotype–phenotype relationships

Aug 8, 2022 5-min Highlight

[Teng-Kuei Huang] A multifunctional system for genome editing and large-scale interspecies gene transfer

[Jemal Ali] Bacterial Competition Systems Share a Domain Required for Inner Membrane Transport of the Bacteriocin Pyocin G from Pseudomonas aeruginosa

[Yun-Chen Cheng] An antibacterial T6SS in Pantoea agglomerans pv. betae delivers a lysozyme-like effector to antagonize competitors

Jul 25, 2022 5-min Highlight

[Yi-Hsuan Hou] Wheat Thioredoxin (TaTrxh1) Associates With RD19-Like Cysteine Protease TaCP1 to Defend Against Stripe Rust Fungus Through Modulation of Programmed Cell Death

[Mao-Sen Liu] Transformation of Rhodococcus Pigment Production Hydroxylase (PPH) gene into Camelina sativa: an alternative marker for the detection of transgenic plants

Jul 11, 2022 5-min Highlight

[Yu Wu] Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

[Li-Kang Sung] A broadly applicable, stress-mediated bacterial death pathway regulated by the phosphotransferase system (PTS) and the cAMP-Crp cascade

[Si-Chong Wang] Shared in planta population and transcriptomic features of nonpathogenic members of endophytic phyllosphere microbiota

Jul 4, 2022 Journal Club (IPMB-PEM)

[Jamal Ali] Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae

Jun 27, 2022 5-min Highlight

[Xuan Lai] Identification of small molecules that strongly inhibit bacterial quorum sensing using a high-throughput lipid vesicle lysis assay

[Erh-Min Lai] Cryo-EM structure of a type IV secretion system

[Yi-Chieh Wang] Subcellular localization of Type VI secretion system assembly in response to cell–cell contact

Jun 13, 2022 5-min Highlight

[Jemal Ali] The MIX domain is required for T6SS-mediated effector secretion

[Yi-Hsuan Hou] Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

[Yung-Hui Wen] Structural insights into ClpP protease side exit pore-opening by a pH drop coupled with substrate hydrolysis

May 30, 2022 5-min Highlight

[Teng-Kuei Huang] Boosting plant genome editing with a versatile CRISPR-Combo system

[Mao-Sen Liou] Simplified floral dip transformation method of Arabidopsis thaliana

May 16, 2022 5-min Highlight

[Yu Wu] YhdP, Skin cells undergo asynthetic fission to expand body surfaces in zebrafish

[Erh-Min Lai] Bacterial species rarely work together

[Erh-Min Lai] Cryo-EM structure of the Agrobacterium tumefaciens T-pilus reveals the importance of positive charges in the lumen

[May 9, 2022 Journal Club (IPMB-PEM)

[Xuan Lai] Motility control through an anti-activation mechanism in Agrobacterium tumefaciens

Apr 25, 2022 5-min Highlight

[Yi-Chieh Wang] Delivery of an Rhs‐family nuclease effector reveals direct penetration of the gram‐positive cell envelope by a type VI secretion system in Acidovorax citrulli

[Li-Kang Sung] T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer

[Xuan Lai] Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae

Apr 11, 2022 5-min Highlight

[Yun-Chen Cheng] YhdP, TamB, and YdbH Are Redundant but Essential for Growth and Lipid Homeostasis of the Gram-Negative Outer Membrane

[Jemal Ali] ClpP inhibitors are produced by a widespread family of bacterial gene clusters

Mar 21, 2022 5-min Highlight

[Teng-Kuei Huang] Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template

[Yi-Hsuan Hou] RGI-GOLVEN signaling promotes cell surface immune receptor abundance to regulate plant immunity

[Mao-Sen Liu] Buy one, get two

[Mar 14, 2022 Journal Club (IPMB-PEM)

[Li-Kang Sung] Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains

Mar 7, 2022 5-min Highlight

[Yu Wu] Microbiome profiling reveals that Pseudomonas antagonises parasitic nodule colonisation of cheater rhizobia in Lotus

[Si-Chong Wang] Rhizosphere bacteriome structure and functions

Feb 14, 2022 5-min Highlight

[Erh-Min Lai] MicroRNA sequence codes for small extracellular vesicle release and cellular retention

[Xuan Lai] The First FRET-Based RNA Aptamer NanoKit for Sensitively and Specifically Detecting c-di-GMP

[Li-Kang Sung] The ectomycorrhizal fungus Pisolithus microcarpus encodes a microRNA involved in cross-kingdom gene silencing during symbiosis

[Yi-Chieh Wang] Inhibiting Type VI Secretion System Activity with a Biomimetic Peptide Designed To Target the Baseplate Wedge Complex

Jan 10, 2022 Journal Club (IPMB-PEM)

[Yun-Chen Cheng] Protective role of the Arabidopsis leaf microbiota against a bacterial pathogen

Jan 3, 2022 5-min Highlight

[Jemal Ali] Mounting, structure and autocleavage of a type VI secretion-associated Rhs polymorphic toxin

[Yun-Chen Cheng] The cost of bacterial predation via Type VI Secretion System leads to predator extinction under environmental stress

Dec 20, 2021 5-min Highlight

[Xuan Lai] A donor-DNA-free CRISPR/Cas-based approach to gene knock-up in rice

[Yi-Chieh Wang] Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants

[Yi-Hsuan Hou] NLRs guard metabolism to coordinate pattern- and effector-triggered immunity

Nov 29, 2021 Journal Club (IPMB-PEM)

[Yi-Hsuan Hou] A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight is Regulated by a Pathogen Effector-Inducible miRNA

Nov 22nd 2021 5-min Highlight

[Mao-Sen Liu] A Single-nucleus RNA sequencing of plant tissues using a nanowell-based system

[Teng-Kuei Huang] Live-cell chromosome dynamics in Arabidopsis thaliana reveals increased chromatin mobility in response to DNA damage

[Yu Wu] Positive interactions are common among culturable bacteria

Nov 8, 2021 5-min Highlight

[Yee-Wai Cheung] A bacterial membrane sculpting protein with BAR domain-like activity

[Erh-Min Lai] Plant immune system activation is necessary for efficient root colonization by auxin-secreting beneficial bacteria

[Si-Chong Wang] Limited peptidoglycan synthesis activates a plant invasion pathway and an envelope stress response in Agrobacterium tumefaciens / The leaf microbiota: responses and impacts on plants

Oct 25, 2021 5-min Highlight

[Jemal Ali] Identification of type VI secretion system toxic effectors using adaptors as markers

[Novaria Sari] Salmonella effector SopD promotes plasma membrane scission by inhibiting Rab10

Oct 18, 2021 Journal Club (IPMB-PEM)

[Teng-Kuei Huang] Ca2+ sensor-mediated ROS scavenging suppresses rice immunity and is exploited by a fungal effector

Sep 27, 2021 5-min Highlight

[Yi-Chieh Wang] The Azospirillum brasilense type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana

[Xuan Lai] Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms

[Yi-Hsuan Hou] A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight Is Regulated by a Pathogen Effector-Inducible miRNA

Sep 13, 2021 5-min Highlight

[Yu Wu] The wasted chewing gum bacteriome

[Mao-Sen Liu] Single-cell RNA-seq analysis reveals ploidy-dependent and cell-specific transcriptome changes in Arabidopsis female gametophytes

[Teng-Kuei Huang] Improved plant cytosine base editors with high editing activity, purity, and specificity

Aug 30, 2021 5-min Highlight

[Yee-Wai Cheung] Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity

[Erh-Min Lai] AgroLux: bioluminescent Agrobacterium to improve molecular pharming and study plant immunity

[Yung-Hui Wen] Identification of Tse8 as a Type VI secretion system toxin from Pseudomonas aeruginosa that targets the bacterial transamidosome to inhibit protein synthesis in prey cells

[Si-Chong Wang] Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction

Aug 23rd 2021 Journal Club (IPMB-PEM)

[Yung-Hui Wen] Burkholderia multivorans requires species-specific GltJK for entry of a contact-dependent growth inhibition system protein

Aug 16, 2021 5-min Highlight

[Jemal Ali] Discovery of a Diverse Set of Bacteria That Build Their Cell Walls without the Canonical Peptidoglycan Polymerase aPBP

[Yun-Chen Cheng] Proteus mirabilis Employs a Contact-Dependent Killing System against Competing Enterobacteriaceae

[Novaria Sari] Host-emitted amino acid cues regulate bacterial chemokinesis to enhance colonization

[Teng-Kuei Huang] Efficient CRISPR-mediated base editing in Agrobacterium spp.

Aug 2nd 2021 5-min Highlight

[Yu Wu] Comparative Analysis of Host-Associated Variation in Phytophthora cactorum

[Mao-Sen Liu] A simple method for in planta tomato transformation by inoculating floral buds with a sticky Agrobacterium tumefaciens suspension

[Si-Chong Wang] A subcellular biochemical model for T6SS dynamics reveals winning competitive strategies

[Si-Chong Wang] Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili

Jul 19, 2021 5-min Highlight

[Teng-Kuei Huang] Agrobacterium VirE2 Protein Modulates Plant Gene Expression and Mediates Transformation From Its Location Outside the Nucleus

[Yi-Chieh Wang] Precise and Reliable Control of Gene Expression in Agrobacterium tumefaciens

Jul 5, 2021 5-min Highlight

[Jemal Ali] Transmembrane domains of type III-secreted proteins affect bacterial-host interactions in enteropathogenic E. coli

[Novaria Sari] Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae

[Erh-Min Lai] Engineered type six secretion systems deliver active exogenous effectors and Cre recombinase

Jun 21, 2021 5-min Highlight

[Yee-Wai Cheung] The small GTPase MglA together with the TPR domain protein SgmX stimulates type IV pili formation in M. xanthus

[Yung-Hui Wen] An Adaptor Hierarchy Regulates Proteolysis during a Bacterial Cell Cycle

[Xuan Lai] Cyclic diguanylate monophosphate directly binds to human siderocalin and inhibits its antibacterial activity

Jun 7, 2021 5-min Highlight

[Mao-Sen Liu] Agrobacterium T-DNA integration in somatic cells does not require the activity of DNA polymerase theta

[Yu Wu] Historical genomics reveals the evolutionary mechanisms behind multiple outbreaks of the host-specific coffee wilt pathogen Fusarium xylarioides

[Si-Chong Wang] Specific modulation of the root immune system by a community of commensal bacteria

May 31, 2021 Journal Club (IPMB-PEM)

[Yee-Wai Cheung] Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa

May 10, 2021 5-min Highlight

[Teng-Kuei Huang] Bacterial effector targeting of a plant iron sensor facilitates iron acquisitionand pathogen colonization

[Yi-Hsuan Hou] Identification of a Putative DNA-Binding Protein in Arabidopsis That Actsas a Susceptibility Hub and Interacts With Multiple Pseudomonas syringae Effectors

[Yun-Chen Cheng] Keeping in Touch with Type-III Secretion System Effectors: Mass Spectrometry-BasedProteomics to Study Effector–Host Protein–Protein Interactions

[Yi-Chieh Wang] Insect egg-killing: a new front on the evolutionary arms-race betweenbrassicaceous plants and pierid butterflies

Apr 26, 2021 5-min Highlight

[Erh-Min Lai] Ecotin and LamB in Escherichia coli influence the susceptibility to TypeVI secretion-mediated interbacterial competition and killing by Vibrio cholerae

[Keith Pintor] Pseudomonas aeruginosa T6SS-mediated molybdate transport contributes to bacterial competition during anaerobiosis

[Novaria Sari] Immunity proteins of dual nuclease T6SS effectors function as transcriptionalrepressors

Apr 19, 2021 Journal Club (IPMB-PEM)

[Novaria Sari] Contact-independent killing mediated by a T6SS effector with intrinsic cell-entryproperties

Apr 12, 2021 5-min Highlight

[Yee-Wai Cheung] A protein-independent fluorescent RNA aptamer reporter system for plant geneticengineering

[Yung-Hui Wen] Genetic Evidence for SecY Translocon-Mediated Import of Two Contact-DependentGrowth Inhibition (CDI) Toxins

[Xuan Lai] Role of the Type VI Secretion System in the Pathogenicity of Pseudomonas syringae pv. actinidiae, the Causative Agent of Kiwifruit Bacterial Canker

Mar 29, 2021 5-min Highlight

[Mao-Sen Liu] 3D imaging of lipid-guided vesicle trafficking along the cytoskeleton

[Si-Chong Wang] Pseudomonas fluorescens F113 type VI secretion systems mediate bacterialkilling and adaption to the rhizosphere microbiome

Mar 15, 2021 5-min Highlight

[Yi-Chieh Wang] The Arabidopsis pattern recognition receptor EFR enhances fire blightresistance in apple

[Yi-Hsuan Hou] Diverse Roles of the Salicylic Acid Receptors NPR1 and NPR3/NPR4 in Plant Immunity

[Yun-Chen Cheng] The chromatin-remodeling protein BAF60/SWP73A regulates the plant immune receptorNLRs

[Erh-Min Lai] Advanced In Vivo Cross-Linking Mass Spectrometry Platform to CharacterizeProteome-Wide Protein Interactions

[Erh-Min Lai] Glucose confers protection to Escherichia coli against contact killing byVibrio cholerae

Mar 5, 2021 5-min Highlight

[Jemal Ali] Phage infection and sub-lethal antibiotic exposure mediate Enterococcus faecalis type VII secretion system dependent inhibition of bystander bacteria

[Keith Pintor] The type VI secretion system of Xanthomonas phaseoli pv. manihotis is involved in virulence and in vitro motility

[Novaria Sari] A novel stabilization mechanism for the type VI secretion system sheath

Feb 22, 2021 Journal Club (IPMB-PEM)

[Keith Pintor] Peptidoglycan editing provides immunity to Acinetobacter baumannii duringbacterial warfare

Feb 1, 2021 5-min Highlight

[Keith Pintor] Characterization of Type VI Secretion System in Xanthomonas oryzae pv.oryzae and Its Role in Virulence to Rice

[Jemal Ali] Contact-independent killing mediated by a T6SS effector with intrinsic cell-entryproperties

[Si-Chong Wang] Highly multiplexed spatial mapping of microbial communities

[Yi-Chieh Wang] Root endophyte induced plant thermotolerance by constitutive chromatinmodification at heat stress memory gene loci

[Mao-Sen Liu] A reporter for noninvasively monitoring gene expression and plant transformation

Jan 18, 2021 5-min Highlight

[Yu Wu] Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virusrestriction factor that suppresses viral transcription

[Yi-Hsuan Hou] CRISPR/Cas9 mutagenesis by translocation of Cas9 protein into plant cells via theAgrobacterium type IV secretion system

[Erh-Min Lai] High-content imaging to phenotype antimicrobial effects on individual bacteria atscale

[Yung-Hui Wen] Modular and coordinated activity of AAA+ active sites in the double-ring ClpAunfoldase of the ClpAP protease

[Xuan Lai] Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer

Jan 11, 2021 Journal Club (IPMB-PEM)

[Mao-Sen Liu] (Progress) An overview of floral dip mediated plant transformation

Jan 7, 2021 5-min Highlight

[Jemal Ali] Structural basis for effector transmembrane domain recognition by type VIsecretion system chaperones

[Mao-Sen Liu] Differential Ubiquitination of BIK1 Fine-Tunes Plant Immunity

[Yun-Chen Cheng] Listeria Phages Induce Cas9 Degradation to Protect Lysogenic Genomes

[Novaria Sari] Endogenous membrane stress induces T6SS activity in Pseudomonas aeruginosa

[Yi-Chieh Wang] F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation

[Si-Chong Wang] Microbial single cell RNA sequencing by split pool barcoding

Dec 21, 2020 5-min Highlight

[Hagos M. Juhar] The MexE/MexF/AmeC Efflux Pump of Agrobacterium tumefaciens and Its Rolein Ti Plasmid Virulence Gene Expression

[Erh-Min Lai] CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genomeengineering

[Keith Pintor] An interbacterial DNA deaminase toxin directly mutagenizes surviving targetpopulations

[Xuan Lai] Unexpected conservation and global transmission of agrobacterial virulenceplasmids

[Novaria Sari] Ticks Resist Skin Commensals with Immune Factor of Bacterial Origin

Nov 30, 2020 5-min Highlight

[Yi-Chieh Wang] Mucin Glycans Signal through the Sensor Kinase RetS to InhibitVirulence-Associated Traits in Pseudomonas aeruginosa

[Mao-Sen Liu] Screening and identification of a non-peptide antagonist for the peptide hormonereceptor in Arabidopsis

[Yun-Chen Cheng] Vibrio cholerae-Symbiont Interactions Inhibit Intestinal Repair inDrosophila

[Si-Chong Wang] A single bacterial genus maintains root growth in a complex microbiome

[Yung-Hui Wen] The Intrinsically Disordered N-terminal Extension of the ClpS Adaptor ReprogramsIts Partner AAA + ClpAP Protease

Nov 16, 2020 5-min Highlight

[Erh-Min Lai] Repeated gain and loss of a single gene modulates the evolution of vascular plantpathogen lifestyles

[Keith Pintor] Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fattyacids and mediate cell egress

[Yu Wu] A single gene in Fusarium oxysporum limits host range

[Yung-Hui Wen] Structural insights into Pseudomonas aeruginosa Type six secretion systemexported effector 8

[Novaria Sari] Burkholderia pseudomallei as an Enteric Pathogen: Identification ofVirulence Factors Mediating Gastrointestinal Infection

[Xuan Lai] A Bacteriophytochrome Mediates Interplay between Light Sensing and the SecondMessenger Cyclic Di-GMP to Control Social Behavior and Virulence

Nov 2, 2020 5-min Highlight

[Jemal Ali] Bacterial nanotubes as a manifestation of cell death

[Mao-Sen Liu] Noninvasive Live ROS Imaging of Whole Plants Grown in Soil

[Yi-Chieh Wang] Blue-light perception by epiphytic Pseudomonas syringae driveschemoreceptor expression, enabling efficient plant infection

[Si-Chong Wang] DNA Uptake upon T6SS-Dependent Prey Cell Lysis Induces SOS Response and ReducesFitness of Acinetobacter baylyi

Oct 19, 2020 5-min Highlight

[Erh-Min Lai] An intrinsic mechanism for coordinated production of the contact-dependent andcontact-independent weapon systems in a soil bacterium

[Hagos M. Juhar] Agrobacterium-delivered VirE2 interacts with host nucleoporin CG1 tofacilitate the nuclear import of VirE2-coated T complex

[Yu Wu] Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI SecretionSystem-Mediated Predation by the Burkholderia cepacia Complex

[Keith Pintor] Targeted Depletion of Bacteria from Mixed Populations by Programmable Adhesionwith Antagonistic Competitor Cells

[Xuan Lai] One gene, multiple ecological strategies: A biofilm regulator is a capacitor forsustainable diversity

Sep 21, 2020 5-min Highlight

[Jemal Ali] Diverse enzymatic activities mediate antiviral immunity in prokaryotes

[Yi-Chieh Wang] The evolution of the type VI secretion system as a disintegration weapon

[Yun-Chen Cheng] Type VI secretion systems of plant‐pathogenic Burkholderia glumae BGR1play a functionally distinct role in interspecies interactions and virulence

Sep 14, 2020 Journal Club (IPMB-PEM)

[Si-Chong Wang] Competition for iron drives phytopathogen control by natural rhizosphere micrbiomes