Apr 8, 2024 5-min Highlight

[Yi-Chieh Wang] Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana

[Yu Wu] Transcriptome profiling of type VI secretion system core gene tssM mutant of Xanthomonas perforans highlights regulators controlling diverse functions ranging from virulence to metabolism

[Hagos M. Juhar] An efficient genetic transformation system mediated by Rhizobium rhizogenes in fruit trees based on the transgenic hairy root to shoot conversion

Mar 25, 2024 5-min Highlight

[Xuan Lai] A PilZ domain protein interacts with the transcriptional regulator HinK to regulate type VI secretion system in Pseudomonas aeruginosa

[Erh-Min Lai] LLPS condensates of Fha initiate the inside-out assembly of the type VI secretion system

[Li-Kang Sung] Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors

Jan 29, 2024 5-min Highlight

[Yung-Hui Wen] Trojan horse-like T6SS effector TepC mediates both interference competition and exploitative competition

[Sin-Sin Hsu] Flagella are required to coordinately activate competition and host colonization factors in response to a mechanical signal

Jan 15, 2024 5-min Highlight

[Mao-Sen Liu] Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution

[Teng-Kuei Huang] Split complementation of base editors to minimize off-target edits

Dec 25, 2023 5-min Highlight

[Yu Wu] Transcriptome architecture of the three main lineages of agrobacteria

[Si-Chong Wang] Genetically refactored Agrobacterium-mediated transformation

[Yi-Chieh Wang] Green leaf volatile sensory calcium transduction in Arabidopsis

[Hagos M. Juhar] A rapid and highly efficient sorghum transformation strategy using GRF4‐GIF1/ternary vector system

Dec 18, 2023 5-min Highlight

[Erh-Min Lai] The mating pilus of E. coli pED208 acts as a conduit for ssDNA during horizontal gene transfer

[Sin-Sin Hsu] The F pilus serves as a conduit for the DNA during conjugation between physically distant bacteria

[Yung-Hui Wen] No fitness cost entailed by type VI secretion system synthesis, assembly, contraction, or disassembly in enteroaggregative Escherichia coli

Dec 6, 2023 5-min Highlight

[Xuan Lai] c-di-GMP inhibits the DNA binding activity of H-NS in Salmonella

[Li-Kang Sung] Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation

Nov 6, 2023 5-min Highlight

[Yi-Hsuan Hou] NLR immune receptor–nanobody fusions confer plant disease resistance

[Mao-Sen Liu] Blueprint for non-transgenic edited plants

[Teng-Kuei Huang] Deletions within intronic T-DNA lead to reversion of T-DNA mutant phenotypes

Oct 30, 2023 Journal Club (IPMB-PEM)

[Yi-Hsuan Hou] Functional diversification of a wild potato immune receptor at its center of origin

Sep 25, 2023 5-min Highlight

[Yu Wu] Time-Course Transcriptome Analysis of Bacillus subtilis DB104 during Growth

[Hagos M. Juhar] Functional diversification of a wild potato immune receptor at its center of origin

[Yi-Chieh Wang] Qualitative and Quantitative Methods to Measure Antibacterial Activity Resulting from Bacterial Competition

[Si-Chong Wang] Ligand-Displaying E. coli Cells and Minicells for Programmable Delivery of Toxic Payloads via Type IV Secretion Systems

Aug 28, 2023 5-min Highlight

[Xuan Lai] Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae

[Erh-Min Lai] Plant extracellular self-DNA inhibits growth and induces immunity via the jasmonate signaling pathway

[Yee-Wai Cheung] A role for the Gram-negative outer membrane in bacterial shape determination

Aug 14, 2023 5-min Highlight

[Sin-Sin Hsu] Elicitor-induced plant immunity relies on amino acids accumulation to delay the onset of bacterial virulence

Jul 24, 2023 Journal Club (IPMB-PEM)

[Hagos M. Juhar] A modified Agrobacterium-mediated transformation for two oomycete pathogens

Jul 3, 2023 5-min Highlight

[Mao-Sen Liu] Early detection of late blight in potato by whole-plant redox imaging

[Teng-Kuei Huang] An efficient CRISPR–Cas12a promoter editing system for crop improvement

Jun 5, 2023 5-min Highlight

[Yu Wu] Shifting microbial communities can enhance tree tolerance to changing climates

[Hagos M. Juhar] Plant commensal type VII secretion system causes iron leakage from roots to promote colonization

[Yi-Chieh Wang] Collective protection against the type VI secretion system in bacteria

[Si-Chong Wang] Specific modulation of the root immune system by a community of commensal bacteria

May 22, 2023 5-min Highlight

[Yee-Wai Cheung] Mechanism of secretion of TcpF by the Vibrio cholerae toxin-coregulated pilus

[Xuan Lai] Programmable protein delivery with a bacterial contractile injection system

[Li-Kang Sung] The accessory protein TagV is required for full Type VI secretion system activity in Serratia marcescens

[Erh-Min Lai] An E1–E2 fusion protein primes antiviral immune signalling in bacteria

[Erh-Min Lai] Ubiquitin-like conjugation by bacterial cGAS enhances anti-phage defence

May 15, 2023 Journal Club (IPMB-PEM)

[Yung-Hui Wen] Membrane-localized expression, production and assembly of Vibrio parahaemolyticus T3SS2 provides evidence for transertion

May 8, 2023 5-min Highlight

[Jemal Ali] The outer membrane and peptidoglycan layer form a single 2 mechanical device balancing turgor

[Sin-Sin Hsu] The Vibrio fischeri type VI secretion system incurs a fitness cost under host-like conditions

Apr 24, 2023 5-min Highlight

[Yu Wu] Back to the Roots: Agrobacterium-Specific Phages Show Potential to Disinfect Nutrient Solution from Hydroponic Greenhouses

[Hagos M. Juhar] The gene TaWOX5 overcomes genotype dependency in wheat genetic transformation

[Mao-Sen Liu] The Chloroplast Envelope of Angiosperms Contains a Peptidoglycan Layer

[Yi-Chieh Wang] Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture

[Teng-Kuei Huang] Induction of T-DNA amplification by retrotransposon-derived sequences

Apr 10, 2023 5-min Highlight

[Yung-Hui Wen] Paradoxical activation of a type VI secretion system (T6SS) phospholipase effector by its cognate immunity protein

Mar 27, 2023 5-min Highlight

[Erh-Min Lai] Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression

[Xuan Lai] A Library of Promoter-gfp Fusion Reporters for Studying Systematic Expression Pattern of Cyclic-di-GMP Metabolism-Related Genes in Pseudomonas aeruginosa

[Li-Kang Sung] Broader functions of TIR domains in Arabidopsis immunity

[Jie Yang] Imaging of Escherichia coli K5 and glycosaminoglycan precursors via targeted metabolic labeling of capsular polysaccharides in bacteria

[Yee-Wai Cheung] A c-di-GMP Binding Effector Controls Cell Size in a Cyanobacterium

Mar 13, 2023 5-min Highlight

[Si-Chong Wang] Community science designed ribosomes with beneficial phenotypes

[Jemal Ali] Specialized pathogenic cells release Tc toxins using a type 10 secretion system

[Yi-Hsuan Hou] Calcineurin B-like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice

[Sin-Sin Hsu] Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions

Mar 2, 2023 5-min Highlight

[Yi-Chieh Wang] Calcium Promotes T6SS-Mediated Killing and Aggregation between Competing Symbionts

[Yu Wu] Multiplicity of Agrobacterium infection of Nicotiana benthamiana for transient DNA delivery

[Teng-Kuei Hwang] evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library

[Mao-Sen Liou] Nanocarrier spray: a nontransgenic approach for crop engineering

Feb 1, 2023 5-min Highlight

[Yu Wu] Gene flow and introgression are pervasive forces shaping the evolution of bacterial species

[Si-Chong Wang] Contrasting patterns of microbial dominance in the Arabidopsis thaliana phyllosphere

Jan 17, 2023 5-min Highlight

[Erh-Min Lai] Activation of ChvG-ChvI regulon by cell wall stress confers resistance to β-lactam antibiotics and initiates surface spreading in Agrobacterium tumefaciens

[Xuan Lai] Bacterial volatile organic compounds attenuate pathogen virulence via evolutionary trade-offs

[Yee-Wai Cheung] A typical Legionella GTPase effector hijacks host vesicular transport factor p115 to regulate host lipid droplet

Jan 9, 2023 Journal Club (IPMB-PEM)

[Teng-Kuei Hwang] The translocated virulence protein VirD5 causes DNA damage and mutation during Agrobacterium-mediated transformation of yeast

Dec 22, 2022 5-min Highlight

[Yung-Hui Wen] The activated ClpP peptidase forcefully grips a protein substrate

[Sin-Sin Hsu] Cryo-EM structure of the Agrobacterium tumefaciens T-pilus reveals the importance of positive charges in the lumen

[Jemal Ali] A secreted effector with a dual role as a toxin and as a transcriptional factor

Dec 8, 2022 5-min Highlight

[Mao-Sen Liou] Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis

[Teng-Kuei Hwang] The translocated virulence protein VirD5 causes DNA damage and mutation during Agrobacterium-mediated transformation of yeast

[Yi-Chieh Wang] Heterologous Assembly of the Type VI Secretion System Empowers Laboratory Escherichia coli with Antimicrobial and Cell Penetration Capabilities

Nov 28, 2022 Journal Club (IPMB-PEM)

[Yu Wu] Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system

Nov 23, 2022 5-min Highlight

[Si-Chong Wang] PSKR1 balances the plant growth-defense trade-off in the rhizosphere microbiome

[Yu Wu] Xanthomonas Infection Transforms the Apoplast into an Accessible and Habitable Niche for Salmonella enterica

[Yi-Hsuan Hou] A conserved protein disulfide isomerase enhances plant resistance against herbivores

Nov 10, 2022 5-min Highlight

[Xuan Lai] Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system

[Erh-Min Lai] A Comprehensive Phenotypic Screening Strategy to Identify Modulators of Cargo Translocation by the Bacterial Type IVB Secretion System

[Li-Lang Sung] Engineering AraC to make it responsive to light instead of arabinose

[Jennifer A.] Mapping the relative abundance of soil microbiome biodiversity from eDNA and remote sensing

Oct 27, 2022 5-min Highlight

[Yung-Hui Wen] Antibacterial T6SS effectors with a VRR-Nuc domain are structure-specific nucleases

[Sin-Sin Hsu] Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context

Oct 17, 2022 Journal Club (IPMB-PEM)

[Mao-Sen Liou] An inducible potato (E,E)-farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco

Oct 5, 2022 5-min Highlight

[Yi-Chieh Wang] Germline transformation of Artemisia annua L. plant via in planta transformation technology “Floral dip”

[Mao-Sen Liou] Functional characterization of a defense-responsive bulnesol/elemol synthase from potato

[Teng-Kuei Hwang] CRISPR/Cas9-mediated generation of fls2 mutant in Nicotiana benthamiana for investigating the flagellin recognition spectrum of diverse FLS2 receptors

Sep 23, 2022 5-min Highlight

[Sin-Sin Hsu] A binary effector module secreted by a type VI secretion system

[Si-Chong Wang] Trade-offs constrain adaptive pathways to T6 survival

[Yu Wu] Trophic interactions between predatory protists and pathogen-suppressive bacteria impact plant health

Aug 22, 2022 5-min Highlight

[Li-Kang Sung] Prokaryotic innate immunity through pattern recognition of conserved viral proteins

[Erh-Min Lai] Mating pair stabilization mediates bacterial conjugation species specificity

Aug 15, 2022 Journal Club (IPMB-PEM)

[Si-Chong Wang] Mapping phyllosphere microbiota interactions in planta to establish genotype–phenotype relationships

Aug 8, 2022 5-min Highlight

[Teng-Kuei Huang] A multifunctional system for genome editing and large-scale interspecies gene transfer

[Jemal Ali] Bacterial Competition Systems Share a Domain Required for Inner Membrane Transport of the Bacteriocin Pyocin G from Pseudomonas aeruginosa

[Yun-Chen Cheng] An antibacterial T6SS in Pantoea agglomerans pv. betae delivers a lysozyme-like effector to antagonize competitors

Jul 25, 2022 5-min Highlight

[Yi-Hsuan Hou] Wheat Thioredoxin (TaTrxh1) Associates With RD19-Like Cysteine Protease TaCP1 to Defend Against Stripe Rust Fungus Through Modulation of Programmed Cell Death

[Mao-Sen Liu] Transformation of Rhodococcus Pigment Production Hydroxylase (PPH) gene into Camelina sativa: an alternative marker for the detection of transgenic plants

Jul 11, 2022 5-min Highlight

[Yu Wu] Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

[Li-Kang Sung] A broadly applicable, stress-mediated bacterial death pathway regulated by the phosphotransferase system (PTS) and the cAMP-Crp cascade

[Si-Chong Wang] Shared in planta population and transcriptomic features of nonpathogenic members of endophytic phyllosphere microbiota

Jul 4, 2022 Journal Club (IPMB-PEM)

[Jamal Ali] Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae

Jun 27, 2022 5-min Highlight

[Xuan Lai] Identification of small molecules that strongly inhibit bacterial quorum sensing using a high-throughput lipid vesicle lysis assay

[Erh-Min Lai] Cryo-EM structure of a type IV secretion system

[Yi-Chieh Wang] Subcellular localization of Type VI secretion system assembly in response to cell–cell contact

Jun 13, 2022 5-min Highlight

[Jemal Ali] The MIX domain is required for T6SS-mediated effector secretion

[Yi-Hsuan Hou] Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

[Yung-Hui Wen] Structural insights into ClpP protease side exit pore-opening by a pH drop coupled with substrate hydrolysis

May 30, 2022 5-min Highlight

[Teng-Kuei Huang] Boosting plant genome editing with a versatile CRISPR-Combo system

[Mao-Sen Liou] Simplified floral dip transformation method of Arabidopsis thaliana

May 16, 2022 5-min Highlight

[Yu Wu] YhdP, Skin cells undergo asynthetic fission to expand body surfaces in zebrafish

[Erh-Min Lai] Bacterial species rarely work together

[Erh-Min Lai] Cryo-EM structure of the Agrobacterium tumefaciens T-pilus reveals the importance of positive charges in the lumen

[May 9, 2022 Journal Club (IPMB-PEM)

[Xuan Lai] Motility control through an anti-activation mechanism in Agrobacterium tumefaciens

Apr 25, 2022 5-min Highlight

[Yi-Chieh Wang] Delivery of an Rhs‐family nuclease effector reveals direct penetration of the gram‐positive cell envelope by a type VI secretion system in Acidovorax citrulli

[Li-Kang Sung] T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer

[Xuan Lai] Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae

Apr 11, 2022 5-min Highlight

[Yun-Chen Cheng] YhdP, TamB, and YdbH Are Redundant but Essential for Growth and Lipid Homeostasis of the Gram-Negative Outer Membrane

[Jemal Ali] ClpP inhibitors are produced by a widespread family of bacterial gene clusters

Mar 21, 2022 5-min Highlight

[Teng-Kuei Huang] Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template

[Yi-Hsuan Hou] RGI-GOLVEN signaling promotes cell surface immune receptor abundance to regulate plant immunity

[Mao-Sen Liu] Buy one, get two

[Mar 14, 2022 Journal Club (IPMB-PEM)

[Li-Kang Sung] Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains

Mar 7, 2022 5-min Highlight

[Yu Wu] Microbiome profiling reveals that Pseudomonas antagonises parasitic nodule colonisation of cheater rhizobia in Lotus

[Si-Chong Wang] Rhizosphere bacteriome structure and functions

Feb 14, 2022 5-min Highlight

[Erh-Min Lai] MicroRNA sequence codes for small extracellular vesicle release and cellular retention

[Xuan Lai] The First FRET-Based RNA Aptamer NanoKit for Sensitively and Specifically Detecting c-di-GMP

[Li-Kang Sung] The ectomycorrhizal fungus Pisolithus microcarpus encodes a microRNA involved in cross-kingdom gene silencing during symbiosis

[Yi-Chieh Wang] Inhibiting Type VI Secretion System Activity with a Biomimetic Peptide Designed To Target the Baseplate Wedge Complex

Jan 10, 2022 Journal Club (IPMB-PEM)

[Yun-Chen Cheng] Protective role of the Arabidopsis leaf microbiota against a bacterial pathogen

Jan 3, 2022 5-min Highlight

[Jemal Ali] Mounting, structure and autocleavage of a type VI secretion-associated Rhs polymorphic toxin

[Yun-Chen Cheng] The cost of bacterial predation via Type VI Secretion System leads to predator extinction under environmental stress

Dec 20, 2021 5-min Highlight

[Xuan Lai] A donor-DNA-free CRISPR/Cas-based approach to gene knock-up in rice

[Yi-Chieh Wang] Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants

[Yi-Hsuan Hou] NLRs guard metabolism to coordinate pattern- and effector-triggered immunity

Nov 29, 2021 Journal Club (IPMB-PEM)

[Yi-Hsuan Hou] A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight is Regulated by a Pathogen Effector-Inducible miRNA

Nov 22nd 2021 5-min Highlight

[Mao-Sen Liu] A Single-nucleus RNA sequencing of plant tissues using a nanowell-based system

[Teng-Kuei Huang] Live-cell chromosome dynamics in Arabidopsis thaliana reveals increased chromatin mobility in response to DNA damage

[Yu Wu] Positive interactions are common among culturable bacteria

Nov 8, 2021 5-min Highlight

[Yee-Wai Cheung] A bacterial membrane sculpting protein with BAR domain-like activity

[Erh-Min Lai] Plant immune system activation is necessary for efficient root colonization by auxin-secreting beneficial bacteria

[Si-Chong Wang] Limited peptidoglycan synthesis activates a plant invasion pathway and an envelope stress response in Agrobacterium tumefaciens / The leaf microbiota: responses and impacts on plants

Oct 25, 2021 5-min Highlight

[Jemal Ali] Identification of type VI secretion system toxic effectors using adaptors as markers

[Novaria Sari] Salmonella effector SopD promotes plasma membrane scission by inhibiting Rab10

Oct 18, 2021 Journal Club (IPMB-PEM)

[Teng-Kuei Huang] Ca2+ sensor-mediated ROS scavenging suppresses rice immunity and is exploited by a fungal effector

Sep 27, 2021 5-min Highlight

[Yi-Chieh Wang] The Azospirillum brasilense type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana

[Xuan Lai] Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms

[Yi-Hsuan Hou] A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight Is Regulated by a Pathogen Effector-Inducible miRNA

Sep 13, 2021 5-min Highlight

[Yu Wu] The wasted chewing gum bacteriome

[Mao-Sen Liu] Single-cell RNA-seq analysis reveals ploidy-dependent and cell-specific transcriptome changes in Arabidopsis female gametophytes

[Teng-Kuei Huang] Improved plant cytosine base editors with high editing activity, purity, and specificity

Aug 30, 2021 5-min Highlight

[Yee-Wai Cheung] Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity

[Erh-Min Lai] AgroLux: bioluminescent Agrobacterium to improve molecular pharming and study plant immunity

[Yung-Hui Wen] Identification of Tse8 as a Type VI secretion system toxin from Pseudomonas aeruginosa that targets the bacterial transamidosome to inhibit protein synthesis in prey cells

[Si-Chong Wang] Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction

Aug 23rd 2021 Journal Club (IPMB-PEM)

[Yung-Hui Wen] Burkholderia multivorans requires species-specific GltJK for entry of a contact-dependent growth inhibition system protein

Aug 16, 2021 5-min Highlight

[Jemal Ali] Discovery of a Diverse Set of Bacteria That Build Their Cell Walls without the Canonical Peptidoglycan Polymerase aPBP

[Yun-Chen Cheng] Proteus mirabilis Employs a Contact-Dependent Killing System against Competing Enterobacteriaceae

[Novaria Sari] Host-emitted amino acid cues regulate bacterial chemokinesis to enhance colonization

[Teng-Kuei Huang] Efficient CRISPR-mediated base editing in Agrobacterium spp.

Aug 2nd 2021 5-min Highlight

[Yu Wu] Comparative Analysis of Host-Associated Variation in Phytophthora cactorum

[Mao-Sen Liu] A simple method for in planta tomato transformation by inoculating floral buds with a sticky Agrobacterium tumefaciens suspension

[Si-Chong Wang] A subcellular biochemical model for T6SS dynamics reveals winning competitive strategies

[Si-Chong Wang] Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili

Jul 19, 2021 5-min Highlight

[Teng-Kuei Huang] Agrobacterium VirE2 Protein Modulates Plant Gene Expression and Mediates Transformation From Its Location Outside the Nucleus

[Yi-Chieh Wang] Precise and Reliable Control of Gene Expression in Agrobacterium tumefaciens

Jul 5, 2021 5-min Highlight

[Jemal Ali] Transmembrane domains of type III-secreted proteins affect bacterial-host interactions in enteropathogenic E. coli

[Novaria Sari] Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae

[Erh-Min Lai] Engineered type six secretion systems deliver active exogenous effectors and Cre recombinase

Jun 21, 2021 5-min Highlight

[Yee-Wai Cheung] The small GTPase MglA together with the TPR domain protein SgmX stimulates type IV pili formation in M. xanthus

[Yung-Hui Wen] An Adaptor Hierarchy Regulates Proteolysis during a Bacterial Cell Cycle

[Xuan Lai] Cyclic diguanylate monophosphate directly binds to human siderocalin and inhibits its antibacterial activity

Jun 7, 2021 5-min Highlight

[Mao-Sen Liu] Agrobacterium T-DNA integration in somatic cells does not require the activity of DNA polymerase theta

[Yu Wu] Historical genomics reveals the evolutionary mechanisms behind multiple outbreaks of the host-specific coffee wilt pathogen Fusarium xylarioides

[Si-Chong Wang] Specific modulation of the root immune system by a community of commensal bacteria

May 31, 2021 Journal Club (IPMB-PEM)

[Yee-Wai Cheung] Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa

May 10, 2021 5-min Highlight

[Teng-Kuei Huang] Bacterial effector targeting of a plant iron sensor facilitates iron acquisitionand pathogen colonization

[Yi-Hsuan Hou] Identification of a Putative DNA-Binding Protein in Arabidopsis That Actsas a Susceptibility Hub and Interacts With Multiple Pseudomonas syringae Effectors

[Yun-Chen Cheng] Keeping in Touch with Type-III Secretion System Effectors: Mass Spectrometry-BasedProteomics to Study Effector–Host Protein–Protein Interactions

[Yi-Chieh Wang] Insect egg-killing: a new front on the evolutionary arms-race betweenbrassicaceous plants and pierid butterflies

Apr 26, 2021 5-min Highlight

[Erh-Min Lai] Ecotin and LamB in Escherichia coli influence the susceptibility to TypeVI secretion-mediated interbacterial competition and killing by Vibrio cholerae

[Keith Pintor] Pseudomonas aeruginosa T6SS-mediated molybdate transport contributes to bacterial competition during anaerobiosis

[Novaria Sari] Immunity proteins of dual nuclease T6SS effectors function as transcriptionalrepressors

Apr 19, 2021 Journal Club (IPMB-PEM)

[Novaria Sari] Contact-independent killing mediated by a T6SS effector with intrinsic cell-entryproperties

Apr 12, 2021 5-min Highlight

[Yee-Wai Cheung] A protein-independent fluorescent RNA aptamer reporter system for plant geneticengineering

[Yung-Hui Wen] Genetic Evidence for SecY Translocon-Mediated Import of Two Contact-DependentGrowth Inhibition (CDI) Toxins

[Xuan Lai] Role of the Type VI Secretion System in the Pathogenicity of Pseudomonas syringae pv. actinidiae, the Causative Agent of Kiwifruit Bacterial Canker

Mar 29, 2021 5-min Highlight

[Mao-Sen Liu] 3D imaging of lipid-guided vesicle trafficking along the cytoskeleton

[Si-Chong Wang] Pseudomonas fluorescens F113 type VI secretion systems mediate bacterialkilling and adaption to the rhizosphere microbiome

Mar 15, 2021 5-min Highlight

[Yi-Chieh Wang] The Arabidopsis pattern recognition receptor EFR enhances fire blightresistance in apple

[Yi-Hsuan Hou] Diverse Roles of the Salicylic Acid Receptors NPR1 and NPR3/NPR4 in Plant Immunity

[Yun-Chen Cheng] The chromatin-remodeling protein BAF60/SWP73A regulates the plant immune receptorNLRs

[Erh-Min Lai] Advanced In Vivo Cross-Linking Mass Spectrometry Platform to CharacterizeProteome-Wide Protein Interactions

[Erh-Min Lai] Glucose confers protection to Escherichia coli against contact killing byVibrio cholerae

Mar 5, 2021 5-min Highlight

[Jemal Ali] Phage infection and sub-lethal antibiotic exposure mediate Enterococcus faecalis type VII secretion system dependent inhibition of bystander bacteria

[Keith Pintor] The type VI secretion system of Xanthomonas phaseoli pv. manihotis is involved in virulence and in vitro motility

[Novaria Sari] A novel stabilization mechanism for the type VI secretion system sheath

Feb 22, 2021 Journal Club (IPMB-PEM)

[Keith Pintor] Peptidoglycan editing provides immunity to Acinetobacter baumannii duringbacterial warfare

Feb 1, 2021 5-min Highlight

[Keith Pintor] Characterization of Type VI Secretion System in Xanthomonas oryzae pv.oryzae and Its Role in Virulence to Rice

[Jemal Ali] Contact-independent killing mediated by a T6SS effector with intrinsic cell-entryproperties

[Si-Chong Wang] Highly multiplexed spatial mapping of microbial communities

[Yi-Chieh Wang] Root endophyte induced plant thermotolerance by constitutive chromatinmodification at heat stress memory gene loci

[Mao-Sen Liu] A reporter for noninvasively monitoring gene expression and plant transformation

Jan 18, 2021 5-min Highlight

[Yu Wu] Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virusrestriction factor that suppresses viral transcription

[Yi-Hsuan Hou] CRISPR/Cas9 mutagenesis by translocation of Cas9 protein into plant cells via theAgrobacterium type IV secretion system

[Erh-Min Lai] High-content imaging to phenotype antimicrobial effects on individual bacteria atscale

[Yung-Hui Wen] Modular and coordinated activity of AAA+ active sites in the double-ring ClpAunfoldase of the ClpAP protease

[Xuan Lai] Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer

Jan 11, 2021 Journal Club (IPMB-PEM)

[Mao-Sen Liu] (Progress) An overview of floral dip mediated plant transformation

Jan 7, 2021 5-min Highlight

[Jemal Ali] Structural basis for effector transmembrane domain recognition by type VIsecretion system chaperones

[Mao-Sen Liu] Differential Ubiquitination of BIK1 Fine-Tunes Plant Immunity

[Yun-Chen Cheng] Listeria Phages Induce Cas9 Degradation to Protect Lysogenic Genomes

[Novaria Sari] Endogenous membrane stress induces T6SS activity in Pseudomonas aeruginosa

[Yi-Chieh Wang] F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation

[Si-Chong Wang] Microbial single cell RNA sequencing by split pool barcoding

Dec 21, 2020 5-min Highlight

[Hagos M. Juhar] The MexE/MexF/AmeC Efflux Pump of Agrobacterium tumefaciens and Its Rolein Ti Plasmid Virulence Gene Expression

[Erh-Min Lai] CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genomeengineering

[Keith Pintor] An interbacterial DNA deaminase toxin directly mutagenizes surviving targetpopulations

[Xuan Lai] Unexpected conservation and global transmission of agrobacterial virulenceplasmids

[Novaria Sari] Ticks Resist Skin Commensals with Immune Factor of Bacterial Origin

Nov 30, 2020 5-min Highlight

[Yi-Chieh Wang] Mucin Glycans Signal through the Sensor Kinase RetS to InhibitVirulence-Associated Traits in Pseudomonas aeruginosa

[Mao-Sen Liu] Screening and identification of a non-peptide antagonist for the peptide hormonereceptor in Arabidopsis

[Yun-Chen Cheng] Vibrio cholerae-Symbiont Interactions Inhibit Intestinal Repair inDrosophila

[Si-Chong Wang] A single bacterial genus maintains root growth in a complex microbiome

[Yung-Hui Wen] The Intrinsically Disordered N-terminal Extension of the ClpS Adaptor ReprogramsIts Partner AAA + ClpAP Protease

Nov 16, 2020 5-min Highlight

[Erh-Min Lai] Repeated gain and loss of a single gene modulates the evolution of vascular plantpathogen lifestyles

[Keith Pintor] Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fattyacids and mediate cell egress

[Yu Wu] A single gene in Fusarium oxysporum limits host range

[Yung-Hui Wen] Structural insights into Pseudomonas aeruginosa Type six secretion systemexported effector 8

[Novaria Sari] Burkholderia pseudomallei as an Enteric Pathogen: Identification ofVirulence Factors Mediating Gastrointestinal Infection

[Xuan Lai] A Bacteriophytochrome Mediates Interplay between Light Sensing and the SecondMessenger Cyclic Di-GMP to Control Social Behavior and Virulence

Nov 2, 2020 5-min Highlight

[Jemal Ali] Bacterial nanotubes as a manifestation of cell death

[Mao-Sen Liu] Noninvasive Live ROS Imaging of Whole Plants Grown in Soil

[Yi-Chieh Wang] Blue-light perception by epiphytic Pseudomonas syringae driveschemoreceptor expression, enabling efficient plant infection

[Si-Chong Wang] DNA Uptake upon T6SS-Dependent Prey Cell Lysis Induces SOS Response and ReducesFitness of Acinetobacter baylyi

Oct 19, 2020 5-min Highlight

[Erh-Min Lai] An intrinsic mechanism for coordinated production of the contact-dependent andcontact-independent weapon systems in a soil bacterium

[Hagos M. Juhar] Agrobacterium-delivered VirE2 interacts with host nucleoporin CG1 tofacilitate the nuclear import of VirE2-coated T complex

[Yu Wu] Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI SecretionSystem-Mediated Predation by the Burkholderia cepacia Complex

[Keith Pintor] Targeted Depletion of Bacteria from Mixed Populations by Programmable Adhesionwith Antagonistic Competitor Cells

[Xuan Lai] One gene, multiple ecological strategies: A biofilm regulator is a capacitor forsustainable diversity

Sep 21, 2020 5-min Highlight

[Jemal Ali] Diverse enzymatic activities mediate antiviral immunity in prokaryotes

[Yi-Chieh Wang] The evolution of the type VI secretion system as a disintegration weapon

[Yun-Chen Cheng] Type VI secretion systems of plant‐pathogenic Burkholderia glumae BGR1play a functionally distinct role in interspecies interactions and virulence

Sep 14, 2020 Journal Club (IPMB-PEM)

[Si-Chong Wang] Competition for iron drives phytopathogen control by natural rhizosphere micrbiomes