|
MICROARRAY SERVICE |
|
|
users provide |
core lab provides |
Note |
|
|
|
instruments available |
technical support
and
consultation |
consumables and reagents |
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Preparation of DNA fragments to be printed:
PCR amplification of ESTs, or DNA clones, PCR product purification,
quality control and re-racking
|
ˇ |
PCR
machines
Liquid handling system
typhoon scanner
microplate
centrifuge
rapid
analysis
gel
electrophoresis
system |
ˇ |
96 well plate tips for liquid handling system
96 well plasmid purification system
Multiscreen PCR
purification plate
3X SSC solution
384 well plate |
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Slide coating：
Home-made poly-lysine slides |
ˇ |
speedvac |
ˇ |
poly-lysine
solution |
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Microarray printing
Calibration printing , test run and microarray printing |
|
GeneMachine
omnigrid
sonicator |
|
home-made polylysine slides
GAPS II coated slides
UltraGAPS slides
Epoxide slides
|
core facility provides microarray printing service |
|
Post-processing of microarray slide |
ˇ |
speedvac
heating
block
oven |
ˇ |
BSA
10% SDS
20X SSC |
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|
Purification and quality control of mRNA |
ˇ |
Agilent bioanalyzer
spectrophotometer
typhoon scanner
Axon
scanner
electrophoresis
system |
ˇ |
RNA Nano-chip 6000
Cy3-dUTP
Cy5-dUTP
mRNA purification
kit
Oligo-dTV primer |
|
|
Cy-dye labeling of mRNA |
ˇ |
|
ˇ |
Cy3-dUTP
Cy5-dUTP
Microcon YM3
column
Cy3 mono-Reactive
Dye
Cy5 mono-Reactive
Dye
Aminoallyl-dUTP
|
|
|
Microarray hybridization |
ˇ |
Hybridization
chamber/station
j GeneTAC
hybridization
system
kMAUI
hybridization
system
lAgilent
hybridization oven
mGeneMachines
HybChambers
water bath
speedvac |
ˇ |
Hybridslip
j
22 X 60
mm
k 22 X 40 mm
l
22 X 22
mm
Agilent Gasket slide
In-situ hybridization kit
(for Agilent DNA chip) |
|
|
Data analysis |
ˇ |
GenePix
pro
GeneSpring |
ˇ |
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