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Institute
of Plant and Microbial Biology, Academia Sinica
DNA Microarray
Core Facility
The DNA microarray core laboratory in the Institute of
Plant and Microbial Biology, Academia Sinica (IPMB), was established in
2001.The core laboratory is dedicated to meeting the needs of genomics
research conducted in IPMB by providing high-throughput, miniature
technologies for plant functional genomics studies. This core laboratory
has also extended its services to the research community at large in
Taiwan. A GeneMachines OmniGrid microarray robot is available for
printing custom-designed DNA microarrays. This instrument will deposit
oligomers or PCR amplified probes onto treated microscope slides using
split pins. Microarray hybridization and data acquisition have been
optimized in the core laboratory with the aid of an automatic
hybridization station and an Axon GenePix scanner. The relative
fluorescent intensity of each spot reveals the expression variation of
the corresponding gene between samples to be compared. The large amount
of data generated from a microarray experiment is analyzed with
statistical techniques then mined using bioinformatics tools. The core
laboratory has to date provide assistance in generating DNA microarrays
for Arabidopsis, rice, lily, redwood, papaya fruit, broccoli,
Phalaenopsis, Hz-1 virus, bamboo, Oncidium,
Antrodia
camphorata ("niu-chang-chih"), yeast, zebrafish,
tomato and human miRNA. In the
post-genomics era, with the aid of this core laboratory, researchers in IPMB and more are exploring the developmental programs in multiple
organisms and extrapolating the results to model building and
biotechnology application.
Committee member:
Dr.
Tuan-hua, Ho
(Chair)
Dr. Long-Fang, Chen
Dr.
Wan-Sheng, Lo
Specialist :
Shu-Jen, Chou
Research assistant:
Ching-I,
Kuo
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