Lin, Na-Sheng (林納生)
Distinguished Research Fellow
- Ph.D., Department of Plant Pathology, University of Nebraska -Lincoln, USA (1984)
- Molecular interactions of plant viruses, satellite RNA and plants
I. Molecular interactions between viruses and host plants
Viruses are obligate parasites that hijack the host cell’s machinery to complete their life cycles after infection. In contrast, plant hormones regulate the defense response, together with gene silencing, to combat virus invasion. In addition, mixed infection of viruses often results in viral synergism. Thus, comparing the defense and counter-defense interaction between viruses and plants is important. We use Bamboo mosaic potexvirus (BaMV) and the two prevalent orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV), as working materials. By integrating next-generation sequencing, genetics, biochemistry, molecular biology, and cellular biology, we aim to identify host factors and microRNAs involved in BaMV replication and movement, analyze the interaction of abscisic acid-induced resistance with gene silencing, and decipher the molecular mechanisms of viral synergism and anti-viral response in Phalaenopsis co-infected with CymMV and ORSV to generate virus-resistant transgenic plants.
II. Trilateral interactions among viruses, satellite RNAs and host plants
Satellite RNAs (satRNAs) are subviral parasites that are almost exclusively associated with plant viruses. Without appreciable sequence homology, satRNAs evolve similar RNA secondary structures to be dependent on their cognate helper viruses for replication and encapsidation. Some satRNAs can interfere in virus replication and virus-induced symptoms. We identified an satRNA (satBaMV) interfering in a BaMV isolate and mapped its key determinants to downregulate BaMV replication. For our long-term goal, we are interested in analyzing whether satBaMV and BaMV share unique or common host factors to complete their life cycles. Recently, we revealed the differential requirement of fibrillarin, a nucleolar protein, for systemic trafficking of satBaMV and BaMV. Our study will provide mechanistic insight into the replication and movement of satRNAs during virus–satRNA–plant interactions.
(A) BaMV atomic model showing N- and C-terminal extensions that allow flexibility of the virions, and left-handed helical with ~8.8 subunits per turn.
(B) Synergistic mixed infection of CymMV and ORSV enhanced symptom formation in Phalaenopsis.
(C) Our research interests focus on trilateral interaction among virus, satellite RNA, and host.
Selected publication list
- Chiang-Hsieh, Y. F., Pai, H., Tseng K. C., Lee S. C., Zheng H. Q., Lin N. S.,* and Chang W. C.* 2017. MicroRPM: An effective model for novel microRNA prediction based only on small RNA sequencing data without reference genome. Bioinformatics. 2017 Nov 9. doi: 10.1093/bioinformatics/btx725. [Epub ahead of print]
- Alazem M, Lin N. S.* 2017. Antiviral Roles of Abscisic Acid in Plants. Front Plant Sci. 8:1760.
- Huang, Y. W., Hu, C.C., Tsai, C.H., Lin, N. S., Hsu, Y.H. 2017. Chloroplast Hsp70 isoform is required for age-dependent tissue preference of Bamboo mosaic virus in mature Nicotiana benthamiana Leaves. Mol Plant Microbe Interact. 30(8): 631-645.
- Huang, Y. W., Hu, C. C., Hsu, Y. H. and Lin, N. S.* 2017. Replication of plant viral satellites. In “Viroids and Plant Viral Satellites” (edited by Drs. Hadidi, Flores, Randles, and Palukaitis), Academic Press (Invited).
- Liou, M. R., Hu, C. C., Lin, N. S. and Hsu, Y. H.* 2017. Development and application of satellite-based vectors. In “Viroids and Plant Viral Satellites” (edited by Drs. Hadidi, Flores, Randles, and Palukaitis), Academic Press (Invited).
- Chang, K. C., Chang, L. T., Huang, Y. W., Lai, Y. C., Lee, C. W., Liao, J. T., Lin, N. S., Hsu, Y. H., Hu, C.C. 2017. Transmission of Bamboo mosaic virus in bamboos mediated by insects in the Order Diptera. Front Microbiol.8:870.
- Wang, I. N.*, Yeh, W. B, Lin, N. S. 2017. Phylogeography and Coevolution of Bamboo Mosaic Virus and Its Associated Satellite RNA. Front Microbiol. 8: 886.
- Chen, T. H., Hu, C. C., Liao, T. L., Lee, Y. L., Huang, Y. W., Lin, N. S., Y. L., Hsu, Y. H.* 2017. Production of Japanese encephalitis virus antigens in plants using bamboo mosaic virus-based vector. Front Microbiol. 8: 788.
- Lin, K. Y.* and Lin, N. S. 2017. Interfering satellite RNAs of Bamboo mosaic virus. Front Microbiol. 8: 787.
- Alazem M, He, M. H., Moffett, P., Lin, N. S.* 2017. Abscisic acid induces resistance against Bamboo mosaic virus through Argonaute 2 and 3. Plant Physiology 174: 339–355.
- Alazem M., and Lin, N. S.* 2017. Large Satellite RNAs. In “Viroids and Plant Viral Satellites” (edited by Drs. Hadidi, Flores, Randles, and Palukaitis), Academic Press (Invited).
- Ho, T. L., Lee, H. C., Chou, Y. L., Tseng, Y. H., Huang ,W. C., Wung, C. H., Lin, N. S., Hsu, Y. H., Chang, B. Y.* 2017. The cysteine residues at the C-terminal tail of Bamboo mosaic virus triple gene block protein 2 are critical for efficient plasmodesmata localization of protein 1 in the same block. Virology 501:47-53.
- Chang, C. H., Hsu, F. C., Lee, S. C., Lo, Y. S., Wang, J. D., Shaw, J., Taliansky, M., Chang, B. Y., Hsu, Y. H., Lin, N. S.* 2016. The nucleolar fibrillarin protein is required for helper virus-independent long-distance trafficking of a subviral satellite RNA in plants. Plant Cell 28:2586-2602.
- Cheng, N., Lo Y. S., Ansari, M. l., Ho, K. C., Jeng, S. T., Lin, N. S.*, Dai, H.* 2016. Correlation between mtDNA complexity and mtDNA replication mode in developing cotyledon mitochondria during mung bean seed germination. New Phytologist doi: 10.1111/nph.14158.
- DiMaio F., Chen, C. C., Yu X., Frenz B., Hsu, Y. H.*, Lin, N. S.*, Egelman E. H.* 2015.The molecular basis for flexibility in the flexible filamentous plant viruses. Nat Struct Mol Biol. 22:642-644.
- Alazem, M., and Lin, N. S.* 2015. Roles of plant hormones in the regulation of host-virus interactions. Mol Plant Pathol. 16:529-540.
- Wang, I. N., Hu,C. C., Lee, C. W., Yen, S. M., Yeh, W. B., Hsu, Y. H., and Lin, N. S.* 2014. Genetic Diversity and Evolution of Satellite RNAs Associated with the Bamboo Mosaic Virus. PLoS ONE 9(9):e108015.
- Liou, M. R., Huang, Y. W., Hu, C. C., Lin, N. S.*, and Hsu, Y. Y.* 2014. A dual gene-silencing vector system for monocot and dicot plants. Plant Biotech. J. 12(3):330-43.
- Alazem, M., Lin, K. Y., and Lin, N. S.* 2014. The abscisic acid pathway has multifaceted effects on the accumulation of Bamboo mosaic virus. Mol Plant-Microbe Interact. 27(2):177-89.
- Lin, K. Y., Hsu, Y. Y., Chen, H. C. and Lin, N. S.* 2013. Transgenic resistance to Bamboo mosaic virus by expressing interfering satellite RNA. Mol. Plant Path. 14: 693-707.
- Chen, H. C., Kong, L. R., Yeh, T. Y., Cheng, C. P., Hsu, Y. H. and Lin, N. S.* 2012. The conserved 5' apical hairpin stem loops of bamboo mosaic virus and its satellite RNA contribute to replication competence. Nucleic Acids Res. 40(10): 4641-4652.
- Lin, N. S. 2012. Development of Bamboo mosaic virus as a plant expression vector for functional study of genes involved in bamboo flowering. In: Tandon, V. and Barik, S. K. Editors, Bamboo flowering and rodent control. pp. 119-123. Astral International Pvt. Ltd. New Delhi.
- Chen, T. Y. and Lin, N. S.* 2011. Transgenic protection against virus infection in orchids. Orchid Biotechnology II. Chapter 18. Edited by H. H. Chen. World Scientific Publishing Co. Pte. Ltd. (Invited)
- Vetten, H. J., Scholthof, K. B. G., Lin, N. S., Briddon, R. W., Ghabrial, S. A., and Palukaitis, P. 2011. Satellites and Other Virus-dependent Nucleic Acids. In: King, A. M. G., Adams, M. J., Carstens, E. B. and Lefkowitz, E. J. Editors, Virus Taxonomy. Oxford: Elsevier, pp. 1209-1220. ISBN: 978-0-12-384684-6. Elsevier Inc. Elsevier. (Invited).
- Vijayapalani, P., Chen, C. F., Liou, M. R., Hsu, Y. H. and Lin, N. S.* 2011. Phosphorylation of Bamboo mosaic virus satellite RNA (satBaMV)-encoded protein P20 downregulates the formation of the satBaMV-P20 ribonucleoprotein complex. Nucleic Acids Res.40(2): 638-649.
Kuan-yu Lin Postdoctoral Fellow
Shu-chuan Lee Postdoctoral Fellow
Jun-da Wang Postdoctoral Fellow
Ning Cheng Postdoctoral Fellow
Yi-chun Chen Postdoctoral Fellow
Alice Liao Research Assistant
Ting-yu Chen Research Assistant
Hsuan Pai Research Assistant
Meng-hsun He Research Assistant
Chih-wei Wang Research Assistant
Xiang-yu Chen Research Assistant
Heng Qian University Student
Yang-sheng Chen University Student