Matzke, Marjori (麥卓琍)
Distinguished Research Fellow
- NIH (National Institutes of Health, USA) Postdoctoral Fellow in Plant Molecular Biology, Washington University, St. Louis, MO, USA; Supervisor: Dr. Mary-Dell Chilton(1980-1982)
- Ph.D. (Honors) University of Kansas, Lawrence, KS, USA, Cell Biology Supervisor: Dr. Robert F. Weaver, Title of dissertation: Role of host RNA polymerases in the transcription of nuclear polyhedrosis virus DNA(1975-1980)
- Oklahoma State University, Bachelor of Science, Natural Sciences(1971-1975)
- Epigenetics: homology-dependent gene silencing, position effects, paramutation, somaclonal variation, RNAi, RNA-directed DNA methylation, transvection
Regulation of gene expression in plants
Our group investigates the regulation of gene expression in plants. Differential expression of gene subsets in specific cell types underpins proper growth, development, and stress responses. The lab has traditionally focused on epigenetic regulation, transcriptional gene silencing and the specific process of RNA-directed DNA methylation. Recently, our findings have led us into a new area, namely alternative splicing of pre-mRNA, which involves regulation at the co- and posttranscriptional levels.
The experimental pipeline employs classical genetic screens to identify putative regulatory proteins, followed by genomics approaches to identify their genomic targets, and molecular/biochemical methodologies to understand their mechanism of action. One current project centers on a system we developed that allows genetic analysis of pre-mRNA splicing in the model plant Arabidopsis thaliana. Mutants retrieved in this screen display either reduced or enhanced expression of an alternatively-spliced GFP reporter gene (Figure 1). So far, the screen has identified several previously uncharacterized factors, some of which may be important for stress responses and hence of interest for agricultural biotechnology. Further analysis of the mutants is likely to uncover additional novel factors that will expand knowledge of alternative splicing and its role in stress adaptation.
We also investigate interphase chromosome organization, which influences gene expression in ways not yet fully understood. Current work involves determining gene expression profiles and DNA methylation patterns in plants containing abnormal numbers of chromosomes (aneuploids), and examining biophysical organizing principles acting on chromosomes, particularly electrical gradients at nuclear membranes. We are presently adapting genetically-encoded fluorescent voltage indicators (GEVIs), developed by neurobiologists to map neural circuits in animals, for ‘light-based’ electrophysiology in plants. GEVIs are advantageous for electrophysiological studies because they can be targeted to internal membranes, such as the nuclear membrane (Figure 2), which are inaccessible in intact cells to classical tools for measuring membrane voltage, such as microelectrodes.
Figure 1. GFP phenotypes of mutant seedlings: left, wild-type T line; middle, GFPweak mutant (reduced expression); right, HyperGFP mutant (enhanced expression)
Figure 2. GEVI localized to inner nuclear membrane of Arabidopsis root cells
Selected publication list
- Matzke AJM, Lin WD, (Matzke M*). Evidence That Ion-Based Signaling Initiating at the Cell Surface Can Potentially Influence Chromatin Dynamics and Chromatin-Bound Proteins in the Nucleus.. Frontiers in Plant Science. 10, 1267, 2019. [JCR] [WOS]
- Kanno T, Lin WD, Chang CL, (Matzke M)*, Matzke AJM*. A Genetic Screen Identifies PRP18a, a Putative Second Step Splicing Factor Important for Alternative Splicing and a Normal Phenotype in Arabidopsis thaliana.. G3-Genes Genomes Genetics. 8(4), 1367-1377, 2018. [JCR] [WOS]
- Hou J, Shi X, Chen C, Islam MS, Johnson AF, Kanno T, Huettel B, Yen MR, Hsu FM, Ji T, Chen PY, (Matzke M), Matzke AJM, Cheng J, Birchler JA. Global impacts of chromosomal imbalance on gene expression in Arabidopsis and other taxa.. Proceedings of the National Academy of Sciences of the United States of America. 115(48), E11321-E11330, 2018. [JCR] [WOS]
- Kanno T, Venhuizen P, Wen TN, Lin WD, Chiou P, Kalyna M, Matzke AJM, (Matzke M). PRP4KA, a Putative Spliceosomal Protein Kinase, Is Important for Alternative Splicing and Development in Arabidopsis thaliana.. Genetics. 210(4), 1267-1285, 2018. [JCR] [WOS]
- Kanno T, Lin WD, Fu JL, Wu MT, Yang HW, Lin SS, Matzke AJM*, and Matzke M* (2016) Identifi cation of coilin
mutants in a screen for enhanced expression of an alternatively spliced GFP reporter gene in Arabidopsis thaliana.
Genetics 203:1709 1720.
- Matzke AJM, Matzke M* (2015) Expression and testing in plants of ArcLight, a genetically-encoded voltage indicator used
in neuroscience research. BMC Plant Biol 15:245.
- Sasaki T, Kanno T, Liang SC, Chen PY, Liao WW, Lin WD, Matzke AJM*, Matzke M*. (2015) An Rtf2 domain-containing
protein influences pre-mRA splicing and is essential for embryonic development in Arabidopsis thaliana. Genetics 200:
- Matzke M, Kanno T, Matzke AJM*. (2015) RNA-directed DNA methylation: the evolution of a complex epigenetic pathway
in flowering plants. Annu Rev Plant Biol 66: 243-267.
- Sasaki T, Lee TF, Liao WW, Naumann U, Liao JL, Eun C, Huang YY, Fu JL, Chen PY, Meyers BC, Matzke AJ*, Matzke M*.
(2014) Distinct and concurrent pathways of Pol II and Pol IV-dependent siRNA biogenesis at a repetitive trans-silencer
locus in Arabidopsis thaliana. Plant J 79: 127-138.
- Huang YY, Lee CP, Fu JL, Chang BC, Matzke AJM*, Matzke M*. (2014) De novo transcriptome sequence assembly from
coconut leaves and seeds with a focus on factors involved in RNA-directed DNA methylation. G3: Genes Genomes
Genetics 4: 3147-2157.
- Matzke MA*, Mosher RA. (2014) RNA-directed DNA methylation: an epigenetic pathway of increasing complexity. Nat Rev
Genet 15: 394-408.
- Matzke AJM*, Matzke M. (2013) Membrane ‘potential-omics’: towards voltage imaging at the cell population level in roots
of living plants. Front Plant Sci 4: 311.
- Lorkovic ZJ, Naumann U, Matzke AJM, Matzke M*. (2012) Involvement of a GHKL ATPase in RNA-directed DNA methylation
in Arabidopsis thaliana. Curr Biol 22: 933-938.
- Kanno T, Bucher E, Daxinger L, Huettel B, Kreil DP, Breinig F, Lind M, Schmitt MJ, Simon SA, Gurazada SG, Meyers
BC, Lorkovic ZJ, Matzke AJM, Matzke M*. (2010) RNA-directed DNA methylation and plant development require an
IWR1-type transcription factor. EMBO Reports 11: 65-71.
- Matzke AJM, Watanabe K, van der Winden J, Naumann U, Matzke M*. (2010) High frequency, cell type-specific visualization
of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants. Plant Methods 6: 2