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Chen, Chung-Mong (陳忠謀)

Emeriti Faculty

  • chen922@gate.sinica.edu.tw
  • Structural and functional analysis of double-stranded telomeric repeat-binding proteins(TRPs) in Arabidopsis

Search for genes affecting the length of telomere at the right end of chromosome III in Arabidopsis thaliana

Telomere-associated sequence (TAS) at the right end of chromosome III (3RTAS) in the ecotype Columbia (Col) of Arabidopsis thaliana differs from that in the ecotype Wassilewskija (Ws) (Wang et al. 2010; Fig 1). Using the difference in 3RTAS as a marker, telomeres 3R in Col (Col-type) and that in Ws ecotype (Ws-type ) can be distinguished from each other in a sample containing genomic DNA from both ecotypes and the length of the corresponding telomere3R can be measured by “single telomere length analysis (STELA) ” technique (Fig 1). The length of Col-type and Ws-type telomere3R in the Arabidopsis population maintained by self-pollination ranges, respectively, from 2 to 4 kb and 4 to 9 kb (Fig 1). To study genes affecting the length of telomere3R in both ecotypes, a single plant of Col was cross-fertilized with that of Ws to generate F1 and F2 and the length of telomere3R was traced from the parents to F2 by STELA (Fig 2). Despite the genetic variation among F1 plants, most of the Col-type and Ws-type telomere3R in F1 plants display a similar length to that of the corresponding parents. It suggests that each one of the Col-type and Ws-type telomere3R may itself carry some information for the maintenance of self-length. Few Col-type or Ws-type telomere3R in the F1 display a length only observed for the telomere3R in the counterpart parents, indicating that some genes recombined between Col and WS genomes can affect the length of both types of telomere3R. In four of the F2 plants carrying both types of telomere3R, all of the Col-type telomere3R and two of the Ws-type telomere3R remain unchanged in length to the corresponding parental telomere3R, while the remaining Ws-type telomere3R display a length as short as the Col-type telomere3R (Fig. 2). It implies that the mechanism for length regulation of Col-type telomere3R differs from that of Ws-type telomere3R. Our data suggests that each telomere in Arabidopsis in the same genome may have its own mechanism to control self-length.

Selected publication list

  • Wang CT, Ho CH, Hseu MJ, and Chen CM.* (2010). The subtelomeric region of the Arabidopsis thaliana chromosome IIIR contains potential genes and duplicated fragments from other chromosomes. Plant Mol. Biol. 74: 155-166.
  • Chen, C. M., Wang, C. T., and Ho, C. H. 2001. A plant gene encoding a Myb-like protein that binds telomeric GGTTTAG repeats in vitro. J. Biol. Chem. 276: 16511-16519.
  • Lin, T. Y., Kao, Y. Y., Lin, S., Lin, R. F., Chen, C. M., Huang, C. H., Wang, C. K., Lin, Y. Z., and Chen, C. C. 2001. Construction of a genetic map for Nicotiana plumbaginifolia and comparison of it with the synteny groups of N. sylvestris. Theor. Appl. Genet. 103: 905-911.
  • Hsu, F. C.,Wang, C. J., Chen, C. M., Hu, H. Y., and Chen, C. C. 2003. Molecular characterization of a family of tandemly repeated DNA sequences, TR-1, in heterochromatic knobs of maize and its relatives. Genetics 164: 1087-1097.
  • Chen, C. M., Wang, C. T., Kao, Y. H., Chang, G. D., Ho, C. H., Lee, F. M., and Hseu, M. J. 2005. Functional reududancy of the duplex telomeric DNA-binding proteins in Arabidopsis. Bot. Bull. Acad. Sinica 46:315-324.
  • Sue, S. C., Hsiao, H. H., Chung, C. P. B., Cheng, Y. H., Hsueh, K. L., Chen, C. M., Ho, C. H., and Huang, T. H. 2006. Solution structure of the DNA binding domain of the telomeric repeat-binding protein of Arabidopsis thaliana: a new fold with an additional C-terminal helix. J. Mol. Biol. 356: 72-85.
  • P.V. Palani, M. Chiu, W. Chen, C.C. Wang, C.C. Lin, C.C. Hsu, C.P. Cheng, Chen. C.M. , Y.H. Hsu, N.S. Lin.. Subcellular localization and expression of bamboo mosaic virus satellite RNA-encoded protein. JOURNAL OF GENERAL VIROLOGY. 90, 507-518., 2009.
  • Wang, C. T., Ho, C. H., Hseu, M. J., and Chen, C. M. 2010. The subtelomeric region of the Arabidopsis thaliana chromosome IIIR contains potential genes and duplicated fragments from other chromosomes. Plant Mol. Biol. 74: 155-166.