Small Molecule Metabolomics Core Lab

Committee Member:

Research Asssistant:

 

The Small Molecule Metabolomics core facility in the Institute of Plant and Microbial Biology was established in April, 2009. Ultra-performance liquid chromatography with Synapt High Definition MS™ (UPLC-HDMS) and Gas chromatography-mass spectrometry offers continuous fast acquisition rates ideal for small molecular metabolomic and derivative analysis. Ultra-performance liquid chromatography with mass detection (UPLC-HDMS) that can separates metabolites in short time (5 times faster than HPLC) is very useful in metabolomics study. Its highly resolution and accuracy can detect the metabolite, and provide the potential elemental composition of the target. The metabolome represents the collection of all metabolites resulting from a wide range of biochemical processes in a biological organism that characterization, identification, and quantitation of metabolites. We provided strategy consultant and technical support like training and workshops for facility users.

Services:

  • Multivariate statistical analysis
  • Accurate determination of the molecular mass of selected compounds.
  • Relative quantitative analysis
  • General unknown screening
  • GC-MS identification and training course
  • UPLC training course
  • HPAEC-PAD training course
  • HPLC training course

The Gas Chromatography-Mass Spectrometry Detector (GC-MSD), purchased at the end of 2009, is able to perform GC-MS identification. The facility also provides consultations for the application of mass- spectrometer in small molecule and the training course for UPLC. For more information, please refer to the AS Metabolomics

Other Link:

AS Metabolomics Core Facility

Facility Reservation system

MassBank
ChemSpider
HMDB Database
LipidMaps
METLIN

Instrument Service Fee Reservation Service mode
Waters SYNAPT G1- HDMS NT 350/30min Facility Reservation system (online) Sample deliver to Mass Facility
Agilent GC-PAL RTC120 /HS/Liq/SPME-MSD 5975C No charge Facility Reservation system User-Operated after training course
Waters ACQUITY UPLC –PDA/ELSD/FLR No charge Facility Reservation system

User-Operated after training course
DIONEX  HPAEC-PAD ICS-5000 No charge Facility Reservation system User-Operated after training course
Agilent 1260 Infinity II HPLC-DAD-FLD-FC No charge Facility Reservation system User-Operated after training course

 

Training Courses Registration schedule

 

Equipments:

•Waters UPLC-SYNAPT G1- HDMS (LC-MS; Ultra-Performance Liquid Chromatography/Mass Spectrometer)
•Agilent GC/MSD 5975C -PAL RTC120 (GC-MS; Gas chromatography -HS/Liq/SPME-mass spectrometry)
•Waters ACQUITY UPLC (UPLC; Ultra-Performance Liquid Chromatography/PDA/ELSD/FLD)
•DIONEX  HPAEC-PAD ICS-5000 (High performance anion exchange chromatography /PAD)
•Agilent 1260 Infinity II HPLC (HPLC; High Performance Liquid Chromatography-DAD)
•Nitrogen concentrator sample evaporator
•Organic solvents vacuum concentration systems