loading

麥東倈 (Matzke, Antonius)

特聘研究員

  • Postdoctoral studies, plant molecular biology, Washington University, St. Louis, supervisor: Dr. Mary-Dell Chilton(1980-1982)
  • Ph.D. Biochemistry, Univ. of Vienna, Austria; Advisor: Dr. Ernst Küchler Dissertation title: Concerning the mechanism of ribosomal translocation(1977-1979)
  • M.S. Biochemistry, Univ. of Vienna, Austria; Advisor: Dr. Ernst Küchler Thesis title: UV-induced cross-linking between the anticodon region of tRNA and mRNA in E. coli ribosomes(1976-1977)
  • B.S. Biochemistry Univ. of Vienna, Austria(1972-1976)
  • +886-2-2787-1137(Lab)
  • +886-2-2787-1136(Office)
  • antoniusmatzke@gate.sinica.edu.tw
  • Interphase chromosome organization in living plants,Influence of aneuploidy on gene expression, chromosome structure and organization,Nuclear membrane proteomics and electrophysiology

研究主題:植物的基因表現調控

本實驗室是在研究調控植物基因表現的因子。不同的基因表現造成的各類型細胞的特化,也造就了生長發育階段的差異及植物對逆境的反應等等。早期的研究是著重在表觀遺傳調控、轉錄基因靜默以及一個特殊的、經由核醣核酸(RNA) 所引導的去氧核醣核酸(DNA)的甲基化過程。近期的研究結果開創了新的研究方向,也就是大家所熟知的「訊息核醣核酸前體的選擇性剪切」(alternative splicing of premRNA)。這個領域包含了RNA轉錄時及轉錄後的調控。

研究策略是由遺傳篩查來找出調控缺失的突變體,再用基因體定序的方式定位突變體中的基因缺失,這些有缺失的基因即為潛在的調控因子,後續再用分生或生化的方式分析這些因子在整個機制中的功能。此遺傳篩查是建立在實驗室已發展出的一套系統----帶有綠色螢光蛋白(GFP) 報導基因的阿拉伯芥轉殖株 ( 圖1) ,此報導基因上游帶有一段會和GFP同時被轉錄的片段,若它必須被正確剪切以轉譯出正常的GFP。在誘導突變之後,我們篩選螢光強度有改變( 變強或變弱) 的突變體。目前利用此策略已篩出先前未知的因子,其中有些可能和植物對逆境的反應相關,這些因子可能會是農業生物技術發展的標的。之後的篩選應當能夠發現更多選擇性剪切或逆境適應的未知分子,讓擴充我們對這些生理現象的知識庫。

細胞間期的染色體組織也是研究主題之一,因為它影響基因表現的機制尚未明瞭。目前階段是觀察非整倍體(aneuploids) 中的基因表現及DNA甲基化的分佈情形,以及多餘的染色體所造成的生物物理現象的改變( 比如細胞核膜電位的改變) 。我們利用神經科學家研發出的電
位指示螢光蛋白(genetically-encoded fluorescentvoltage indicators, GEVIs) 來設計以螢光為訊號的植物電生理研究。因為GEVIs能夠被表現在細胞的內膜細統裡( 圖2) ,這使得以往深藏在細胞裡、難以用傳統電生理儀器測量的內膜系統電位得以被研究。

Figure 1. GFP phenotypes of mutant seedlings: left, wild-type T line; middle, GFPweak mutant (reduced expression); right, HyperGFP mutant (enhanced expression)

Figure 2. GEVI localized to inner nuclear membrane of Arabidopsis root cells

All publication list

Selected publication list

  • Matzke AJM, Lin WD, (Matzke M*). Evidence That Ion-Based Signaling Initiating at the Cell Surface Can Potentially Influence Chromatin Dynamics and Chromatin-Bound Proteins in the Nucleus.. Frontiers in Plant Science. 10, 1267, 2019. [JCR] [WOS]
  • Kanno T, Lin WD, Chang CL, (Matzke M)*, Matzke AJM*. A Genetic Screen Identifies PRP18a, a Putative Second Step Splicing Factor Important for Alternative Splicing and a Normal Phenotype in Arabidopsis thaliana.. G3-Genes Genomes Genetics. 8(4), 1367-1377, 2018. [JCR] [WOS]
  • Hou J, Shi X, Chen C, Islam MS, Johnson AF, Kanno T, Huettel B, Yen MR, Hsu FM, Ji T, Chen PY, (Matzke M), Matzke AJM, Cheng J, Birchler JA. Global impacts of chromosomal imbalance on gene expression in Arabidopsis and other taxa.. Proceedings of the National Academy of Sciences of the United States of America. 115(48), E11321-E11330, 2018. [JCR] [WOS]
  • Kanno T, Venhuizen P, Wen TN, Lin WD, Chiou P, Kalyna M, Matzke AJM, (Matzke M). PRP4KA, a Putative Spliceosomal Protein Kinase, Is Important for Alternative Splicing and Development in Arabidopsis thaliana.. Genetics. 210(4), 1267-1285, 2018. [JCR] [WOS]
  • Kanno T, Lin WD, Fu JL, Wu MT, Yang HW, Lin SS, Matzke AJM*, and Matzke M* (2016) Identifi cation of coilin
    mutants in a screen for enhanced expression of an alternatively spliced GFP reporter gene in Arabidopsis thaliana.
    Genetics 203:1709 1720.
  • Matzke AJM, Matzke M* (2015) Expression and testing in plants of ArcLight, a genetically-encoded voltage indicator used
    in neuroscience research. BMC Plant Biol 15:245.
  • Sasaki T, Kanno T, Liang SC, Chen PY, Liao WW, Lin WD, Matzke AJM*, Matzke M*. (2015) An Rtf2 domain-containing
    protein influences pre-mRA splicing and is essential for embryonic development in Arabidopsis thalianaGenetics 200:
    523-535.
  • Matzke M, Kanno T, Matzke AJM*. (2015) RNA-directed DNA methylation: the evolution of a complex epigenetic pathway
    in flowering plants. Annu Rev Plant Biol 66: 243-267.
  • Sasaki T, Lee TF, Liao WW, Naumann U, Liao JL, Eun C, Huang YY, Fu JL, Chen PY, Meyers BC, Matzke AJ*, Matzke M*.
    (2014) Distinct and concurrent pathways of Pol II and Pol IV-dependent siRNA biogenesis at a repetitive trans-silencer
    locus in Arabidopsis thalianaPlant J 79: 127-138.
  • Huang YY, Lee CP, Fu JL, Chang BC, Matzke AJM*, Matzke M*. (2014) De novo transcriptome sequence assembly from
    coconut leaves and seeds with a focus on factors involved in RNA-directed DNA methylation. G3: Genes Genomes
    Genetics 4: 3147-2157.
  • Matzke MA*, Mosher RA. (2014) RNA-directed DNA methylation: an epigenetic pathway of increasing complexity. Nat Rev
    Genet 15: 394-408.
  • Matzke AJM*, Matzke M. (2013) Membrane ‘potential-omics’: towards voltage imaging at the cell population level in roots
    of living plants. Front Plant Sci 4: 311.
  • Lorkovic ZJ, Naumann U, Matzke AJM, Matzke M*. (2012) Involvement of a GHKL ATPase in RNA-directed DNA methylation
    in Arabidopsis thalianaCurr Biol 22: 933-938.
  • Kanno T, Bucher E, Daxinger L, Huettel B, Kreil DP, Breinig F, Lind M, Schmitt MJ, Simon SA, Gurazada SG, Meyers
    BC, Lorkovic ZJ, Matzke AJM, Matzke M*. (2010) RNA-directed DNA methylation and plant development require an
    IWR1-type transcription factor. EMBO Reports 11: 65-71.
  • Matzke AJM, Watanabe K, van der Winden J, Naumann U, Matzke M*. (2010) High frequency, cell type-specific visualization
    of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants. Plant Methods 6: 2
Tatuso Kanno
菅野達夫
Assistant Research Specialist
研究助技師
Chia-liang Chang
張家亮
Research Assistant
資深研究助理
Phebe Hui Chiou
邱蕙
Research Assistant
研究助理

 
Christine Ya-Ching Ying
應雅青
Lab Manager
實驗室助理