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鍾美珠 (Chung, Mei-Chu)

研究員

  • 博士 國立台灣大學 (1988)
  • 碩士 國立臺灣大學農藝研究所 (1980)
  • 學士 國立臺灣大學農藝系 (1978)
  • +886-2-2787-1010(Lab)
  • +886-2-2787-1153(Office)
  • bomchung@gate.sinica.edu.tw
  • 分子細胞遺傳

 

稻屬(Oryza)中約有24個物種,其中有兩個是栽培稻,其餘為野生稻。利用遠緣雜交及後續回交多次可以將野生稻的抗病蟲害和抗逆境等重要的農藝性狀導入栽培品種,但是育種成效往往受限於雜交後代的低稔性甚至於不稔性。在稻屬內,各物種的染色體數目和染色體結構都相當保守,但rDNA-FISH的結果顯示45S rDNA基因座的數目和分佈在物種間頗有差異。因此推論在演化過程中,某些rDNA基因座曾發生大規模的基因擴增或刪除,甚至基因座移位或其所在的染色體片段發生反轉。此外,在兩個遠緣且基因組大小懸殊的二倍體野生稻Oryza australiensis (EE)和O. brachyantha (FF)的45S rDNA之基因間非轉錄區(IGS)之序列和編組與稻屬內其他物種迥異,顯然經歷不同的演化途徑。利用基因組原位雜合技術(genomic in situ hybridization, GISH)標記來自不同水稻物種的染色體,發現種間雜交後代的來自不同物種的染色體在花粉母細胞減數分裂時鮮少配對,多數染色體為單價體形式,偶爾可見異源配對或同源配對所形成的二價體或多價體。減數分裂所產生的配子體所包含的染色體數目和組成極為不均,說明水稻遠緣種間雜交F1高度不稔的原由。遠緣雜交種在減數分裂時染色體的配對行為可說明來自兩親的染色體是否有聯會、互換、發生基因組重組的機會。觀察水稻遠緣種間雜交F1之減數分裂時近同源染色體間的配對行為並探討水稻物種間的雜交親和性,為育種工作重要的參考資訊,藉此也可以瞭解物種間基因組的編組和結構差異,亦可探討稻屬物種之基因組多樣性和演化親緣關係。

All publication list

Selected publication list

  • Lee YI, Chen MC, Lin L, Chung MC*, Leu WM* (2018) Increased expression of 9-cis-epoxycarotenoid dioxygenase, PtNCED1, associated with inhibited seed germination in a terrestrial orchid, Phaius tankervilliae. Front. Plant Sci. 9:1043. doi: 10.3389/fpls.2018.01043
  • Chang LY, Li KT, Yang WJ, Chung MC, Chang JC, Chang MW (2018) Ploidy level and their relationship with vegetative traits of mulberry (Morus spp.) species in Taiwan. Scientia Horticulturae 235 (2018) 78–85
  • Lee YI, Chung MC, Kuo HC, Wang CN, Lee YC, Lin CY, Jiang H, Yeh CH (2017) The evolution of genome size and distinct distribution patterns of rDNA in Phalaenopsis (Orchidaceae). Bot J Linn Soc 185: 65–80.
  • Lee YI, Chung MC, Sydara K, Souliya O, Aphay SL (2017) Taxonomic placement of Paphiopedilum rungsuriyanum (Cypripedioideae; Orchidaceae) based on morphological, cytological and molecular analyses. Bot Stud 58:16
  • Lee YI, Chang FC, Chung MC (2016) Distinct distribution patterns of 45S rDNA and 5SrDNA-NTS-related repeats display diverse karyotypes in Paphiopedilum. In: Orchid Biotechnology III, WH Chen and HH Chen (eds.), New Jersey, World Scientific, 2016. Pp23-50.
  • Chung MC* (2015) Chromosome techniques and FISH. (eds.) In: Plant Microtechniques and Protocols, ECT Yeung et al., (eds.), Springer International Publishing Switzerland. pp. 287-309.
  • Lee YI, Chung MC (2015) Abscisic acid immunostaining. In: Plant Microtechniques and Protocols, ECT Yeung et al., (eds.), Springer International Publishing Switzerland. pp. 251-261.
  • Lee YI, Chung MC, Yeung E, Lee N (2015) Dynamic distribution and the role of abscisic acid during seed development of a lady's slipper orchid, Cypripedium formosanum. Annals of Botany 116: 403–411.
  • Lo KL, Wang LC, Chen J, Liu YC, Chung MC, Lo WS (2014) Transcriptional Consequence and Impaired Gametogenesis with High-Grade Aneuploidy in Arabidopsis thaliana. PLoS ONE 9: e114617.
  • Liu MC, Yang CS, Yeh Fl, Wei CH, Jane WN, Chung MC, Wang CS (2014) A novel lily anther-specific gene encodes adhesin-like proteins associated with exine formation during anther development. Journal of Experimental Botany. 65:2023-2037.
  • Chang YC, Shii CT, Lee YC, Chung MC* (2013) Diverse chromosome complements in the functional gametes of interspecific hybrids of MT- and A-karyotype Lycoris spp. Plant Systematics and Evolution. 299:1141–1155.
  • Fang SA, Eu TI, Chung MC* (2011) Isolation and characterization of genome-specific markers in Oryza species with the BB genome. Plant Science 181:300–308
  • Lee YI, Chang FC, Chung MC* (2011) Chromosome paring affinities in interspecific hybrids reflecting the phylogenetic distances among the lady’s slipper orchids. Annals of Botany 108:113–121.

博士後研究 Postdoctoral Fellow

劉茂森 Mao-Sen Liu

研究助理 Research Assistant

曾詩軒 Shih-Hsuan Tseng