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馮騰永 (Feng, Teng-Yung)

退休研究員

  • natfarm@sinica.edu.tw
  • 植物抗病性之研究發展與應用

植物抗病性之研究發展與應用

本實驗室長期從事茄科植物與植物病原微生物交互作用的研究,發現在甜椒中有兩種會影響植物過敏反應發生的植物蛋白,分別命名為PFLP與HRAP。這兩種蛋白質在與細菌的致敏蛋白harpinPss混合接種之後,均能在接種植物的細胞間隙中促進harpinPss所引發的過敏反應。在經過基因選殖與許多的定性分析之後,我們發現這兩種蛋白及其基因的重要性質如下:

PFLP (Plant Ferredoxin-Like Protein)
PFLP是屬於Ferredoxin(硫鐵蛋白)一類的蛋白質,位於植物細胞內的葉綠體中。植物病原細菌的接種會壓抑植物光合作用硫鐵蛋白的產生(圖一),並減弱植物的抗病力。在轉殖菸草的實驗中顯示,當致敏原刺激時(如harpinPss接種),增加PFLP的表現量能增加致敏原所引發的過氧化物,進而降低植物細胞表現過敏反應之細胞死亡的門檻。藉由這種機制,許多原本會在菸草上致病的病原菌(包括Pseudomonas屬與Erwinia屬之細菌性病原菌,以及Botrytis屬之真菌性病原菌),在pflp的轉殖菸草上卻引起抗病的過敏反應,而使其無法寄生,造成轉基因植物對植物病害更具抗性。這項發現提供農作物在選育抗病品種時一項有效策略之應用。根據此項發現我們已獲得抗白葉枯病的水稻、抗軟腐病的青花菜、抗青枯病與軟腐病的蕃茄(圖二)以及抗軟腐病的彩色海芋(圖三)等。該基因並於2006年8月11日,本著人道協助非洲農民解決香蕉萎凋病之精神,由中央研究院與非洲農業技術基金會簽署授權合約,期能協助非洲農民解決香蕉生產困境,改善糧食問題。

HRAP (Hypersensitive Response Assisting Protein)
HRAP的基因hrap是一個新發現的基因,與已知功能的蛋白或基因序列均不相似。依序列的分析顯示,HRAP極可能位於植物細胞表面,而有機會與病原菌所分泌的分子發生交互作用。在生化實驗中,HRAP蛋白能解離harpinPss的多聚體,使得harpin蛋白在植物細胞上更容易引起過敏反應。另一方面,HRAP蛋白也可在植物細胞間隙中降低細菌性病原菌的致病力。在天然甜椒植株中,hrap是要在非親合性病原菌的感染之下才會被誘發表現。但在hrap轉殖菸草中,HRAP的持續表現,造成轉殖植物對Pseudomonas屬,Xanthomonas屬與Erwinia屬等細菌性病原菌都具有效的抗性。 


pflp
hrap兩個基因在多種植物抗病性上的應用以及在逆境下的反應,正在多方面的進行中。而pflp在抗病上的應用,已獲得歐美與本地的專利。

Selected publication list

  • Hsiang-En Huang, Chien-An Liu, Mei-Jiuan Lee, C-George Kuo, Huei-Mei Chen, Mang-Jye Ger, Yu-Chih Tsai, Yen-Ru Chen, Ming-Kun Lin, and T-Y Feng 2007. Resistance enhancement of transgenic tomato to bacterial pathogens by the heterologous expression of sweet pepper ferredoxin-I protein (PFLP). Phytopathology 97:900-906.
  • Yip MK,Huang HE,Ger MJ, Chen CY, Chiu SH, Tsai YC, Lin CI and TY. Feng. 2007. Production of soft rot resistant cally lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants. Plant Cell Report 26:449-457.
  • Huang HE,Ger MJ, Chen CY, Pandey AK, Yip MK, Chou HW and TY. Feng. 2007. Disease resisitance to bacterial pathogen effected by the qunatity of ferredoxin-I protein in plants. Molecular Plant pathology 8:129-137.
  • Tang, K.X., Sun, X.F., Hu, Q.N., Wu, A.Z., Lin, C.H., Lin, H.-J., Twyman, R.M., Christou, P., and Feng, T.-Y.(2001).
    Trangenic rice plants expressing the ferredoxin-like proein (AP1) from sweet pepper show enhanced resistance to Xanthomonas oryzae pv. oryzae. Plant Sci. 160, 1035-1042.
  • Podile, A.R., Lin, H.J., Stainforth, V., Sripriya, P., Chen, L.-F.O., and Feng, T.-Y.(2001).
    Conditional expression of harpinpss causes yeast cell death that shares features of cell death pathway with harpinpss-mediated hypersensitive response (HR). Physiol. Mol. Plant Pathol. 58, 267-276.
  • Dayakar, B.V., Lin, H.-J., Chen, C.-H., Ger, M.-J., Lee, B.-H., Pai, C.-H., Chow, D., Huang, H.-E., Hwang, S.-Y., Chung, M.-C., and Feng, T.-Y.(2003).
    Ferredoxin from sweet pepper (Capsicum annuum L.) intensifying harpinpss-mediated hypersensitive response shows an enhanced production of active oxygen species (AOS). Plant Mol. Biol. 51, 913-924.
  • Ger, M.-J., Chen, C.-H., Hwang, S.-Y., Huang, H.-E., Podile, A.R., Dayakar, B.V., and Feng, T.-Y.(2003).
    Constitutive expression of hrap gene in transgenic tobacco plant enhances resistance against virulent bacterial pathogens by induction of a hypersensitive response. Mol. Plant-Microbe Interact. 15, 764-773.
  • Liau, C.-H., Lu, J.-C., Prasad, V., Hsiao, H.-H., You, S.-J., Lee, J.-T., Yang, N.-S., Huang, H.-E., Feng, T.-Y., Chen, W.-H., and Chan, M.-T.(2003).
    The sweet pepper ferredoxin-like protein (pflp) conferred resistance against soft rot disease in Oncidium orchid. Transgenic Res. 12, 329-336
  • Lu, J.-C., Liau, C.-H., Prasad, V., Lee, J.-T., Hsiao, H.-H., You, S.-J., Yang, N.-S., Huang, S.-E., Feng, T.-Y., Chen, W.-H., and Chan, M.-T.(2003).
    Oncidium orchid ectopically expressing the sweet pepper ferredoxin-like protein (AP1) conferred resistance against soft rot disease. Transgenic Res. 12, 329-336.
  • You, S.-J., Liau, C.-H., Huang, H.-E., Feng, T.-Y., Prasad, V., Hsiao, H.-H., Lu, J.-C., and Chan, M.-T.(2003).
    Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation. Planta 217, 60-65.
  • Huang, H.-E., Ger, M.-J., Yip, M.-K., Chen, C.-Y., Pandeya, A.-K., and Feng, T.-Y.(2004).
    A hypersensitive response was induced by virulent bacteria in transgenic tobacco plants overexpressing a plant ferredoxin-like protein (PFLP). Physiol. Mol. Plant Pathol. 64, 103-110.
  • Pandey, A.-K., Ger, M.-J., Huang, H.-E., Yip, M.-K., Zeng, J., and Feng, T.-Y.(2005).
    Expression of the hypersensitive response-assisting protein in Arabidopsis results in harpin-dependent hypersensitive cell death in response to Erwinia carotovora. Plant Mol. Biol. 59, 771-780.
  • Huang, H.-E., Ger, M.-J., Chen, C.-Y., Yip, M.-K., Chung, M.-C., and Feng, T.-Y.(2006).
    Plant ferredoxin-like protein (PFLP) exhibits an anti-microbial ability against soft-rot pathogen Erwinia carotovora subsp. carotovora in vitro and in vivo. Plant Sci. 171, 17-23.
  • Huang, H.-E., Ger, M.-J., Chen, C.-Y., Pandey, A.-K., Yip, M.-K., Chou, H.-W., and Feng, T.-Y.(2007).
    Disease resistance to bacterial pathogens affected by the amount of ferredoxin-I protein in plants. Mol. Plant Pathol. 8, 129-137.
  • Huang, H.-E., Liu, C.-A., Lee, M.-J., Kuo, C.-G., Chen, H.-M., Ger, M.-J., Tsai, Y.-C., Chen, Y.-R., Lin, M.-K., and Feng, T.-Y.(2007).
    Resistance enhancement of transgenic tomato to bacterial pathogens by the heterologous expression of sweet pepper ferredoxin-I protein. Phytopathol. 97,900-906.
  • Yip, M.-K., Huang, H.-E., Ger, M.-J., Chiu, S.-H., Tsai, Y.-C., Lin, C.-I., and Feng, T.-Y. (2007).
    Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants. . Plant Cell Rep. 26, 449-457.

Patents

  • Lin, H.-J., and FENG, T.-Y. (1999. Oct.).
    The use of a ferredoxin-like gene to protect plants against bacterial diseases. (USA patent) patent no. 5968804
  • Lin, H.-J., and FENG, T.-Y.(2001. Oct 2.).
    The use of a ferredoxin-like gene to protect plants against bacterial diseases. (USA patent) patent no. 6297360.
  • Lin, H.-J., and Feng, T.-Y. (2002. Sep. 13.).
    Amphipathic protein-1. (Netherlands patent) patent no. 1011688.