Wu, Shu-Hsing

吳素幸
Wu, Shu-Hsing
Research Fellow

Ph.D., Plant Biology, University of California, Davis, USA (1997)

TEL: 

+886-2-2787-1178 (Office)
+886-2-2787-1066 (Lab)

Light-mediated gene expression and signal transduction in Arabidopsis

“Light” regulates multiple aspects of growth and development in plants. Plants have a collection of photoreceptors to interpret and transduce the light signals. The light-mediated morphogenesis is the conclusion of gene expression and interactions. Light influences gene expression at multiple levels, from transcription to post-translational modification. When the expression and interactions of genes are harmonically coordinated, plants achieve optimal growth and development under ever-changing light environment. Our primary goal is to identify genes and regulatory mechanisms for gene expression associated with lightregulated plant growth and development.

Our studies have revealed that, in addition to the commonly investigated transcriptional regulation and selective protein degradation, post-transcriptional regulation plays an important role in Arabidopsis photomorphogenic development. Small RNAs function in maintaining adequate mRNA abundance in cells and alternative splicing increases transcript and proteome diversities. We have also observed a clear impact of light on selective protein translation.

Light also sets the circadian clock in plants for them to coordinate internal biochemical processes for the anticipation of the 24-h light-dark cycles. The circadian clock in Arabidopsis consists of several interlocked feedback loops, yet additional regulatory components remain to be discovered. We found that LWD1 and LWD2 are two clock proteins essential for the stabilization and robustness of Arabidopsis circadian clock.

Our laboratory will continue to discover hidden components in light signaling pathways. We will also dedicate our efforts in decoding the action mechanisms of the components identified.

 

All publication list

 

Selected publication list

 

  • Huang CF, Yu CP, Wu YH, Lu MJ, Tu SL, Wu SH, Shiu SH, Ku MSB and Li WH* (2017) Elevated auxin biosynthesis and transport underlie high vein density in C4 leaves. Proc Natl. Acad. Sci. USA 114: E6884-E6891

  • Lin MC, Tsai HL, Lim SL, Jeng ST and Wu SH* (2017) Unraveling multifaceted contributions of small regulatory RNAs to photomorphogenic development in Arabidopsis. BMC Genomics. 18: 559

  • Huang WY, Wu YC, Pu HY, Wang Y, Jang GJ and Wu SH* (2017) Plant dual-specificity tyrosine phosphorylation-regulated kinase optimizes light-regulated growth and development in Arabidopsis. Plant Cell Environ. 40: 1735-1747

  • Li C, Sako Y, Imai A, Nishiyama T, Thompson K, Kubo M, Hiwatashi Y, Kabeya Y, Karlson D, Wu SH, Ishikawa M, Murata T, Benfey P.N, Sato Y, Tamada Y, Hasebe M* (2017) A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens. Nat. Commun. 8: 14242

  • Wu J.-F., Tsai H.-L., Joanito I., Wu Y.-C., Chang C.-W., Li Y.-H., Wang Y., Hong J. C., Chu J.-W., Hsu C.-P., and Wu S.-H. (2016) LWD-TCP complex activates the morning gene CCA1 in Arabidopsis. Nat. Commun. 7, 13181 doi: 10.1038/ncomms13181

  • 2.Huang C.-F., Chang Y.-M., Lin J.-J., Yu C.-P., Lin H.-H., Liu W.-Y., Yeh S., Tu S.-L., Wu S.-H., Ku M. S.-B.*, and Li W.-H.* (2016) Insights into the regulation of C4 leaf development from comparative transcriptomic analysis. Curr. Opin. Plant Biol. 30: 1-10

  • Lee W.-C., Lu S.-H., Lu M.-H., Yang C.-J., Wu S.-H., Chen H.-M* (2015) Asymmetric bulges and mismatches determine 20-nucleotide microRNA formation in plants. RNA Biol. 12: 1054-66

  • Yu C.-P., Chen S. C.-C., Chang Y.-M., Liu W.-Y., Lin H.-H., Lin J.-J., Chen H.-J., Lu Y.-J., Wu Y.-H., Lu M.-Y. J., Lu C.-H., Shih A. C.-C., Ku M. S.-B., Shiu S.-H.*, Wu S.-H.*, Li W.-H.* (2015) Transcriptome dynamics of developing maize leaves and genome-wide prediction of cis elements and their cognate transcription factors. Proc. Natl. Acad. Sci. USA 112: E2477-86

  • Tsai H.-L., Li Y.-H., Hsieh W.-P., Lin M.-C., Ahn J. H., Wu S.-H.* (2014) HUA ENHANCER 1 is involved in post-transcriptional regulation of positive and negative regulators in Arabidopsis photomorphogenesis. Plant Cell 26: 2858-2872

  • Wu H.-Y., Liu K.-H., Wang Y.-C., Wu J.-F., Chiu W.-L., Chen C.-Y., Wu S.-H., Sheen J., Lai E.-M.* (2014) AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings. Plant Methods 10:19

  • Wu S.-H.* (2014) Gene expression regulation in photomorphogenesis: from the perspective of central dogma. Ann. Rev. Plant Biol. 65:311-333

  • Liu M.-J., Wu S.-H., Wu J.-F., Lin W.-D., Wu Y.-C., Tsai T.-Y, Tsai H.-L., Wu S.-H.* (2013) Translational landscape of photomorphogenic Arabidopsis. Plant Cell 10: 3699-710

  • Chen C.-E., Yeh K.-C., Wu S.-H., Wang H.-I., Yeh H.-H.* (2013) A vicilin-like seed storage protein, PAP85, is involved in tobacco mosaic virus replication. J Virol. 87:6888-68900

  • Chen YY, Wang Y, Shin LJ, Wu JF, Shanmugam V, Tsednee M, Lo JC, Chen CC, Wu SH, and Yeh KC.* (2013) Iron is involved in maintenance of circadian period length in Arabidopsis. Plant Physiol. 161:1409-1420.

  • Hsieh WP, Hsieh HL, Wu SH.* (2012) Arabidopsis bZIP16 transcription factor integrates light and hormone signaling pathways to regulate early seedling development. Plant Cell 24: 3997–4011

  • Liu MJ, Wu S, Chen HM, Wu SH.* (2012) Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis. Molecular Systems Biology 8:566.

  • Chang CS, Maloof JN, Wu SH.* (2011) COP1-mediated degradation of BBX22/LZF1 optimizes seedling development in Arabidopsis. Plant Physiol. 156:228-239.

  • Wang Y, Wu JF, Nakamichi N, Sakakibara H, Nam HG, and Wu SH.* (2011) LIGHT-REGULATED WD1 and PSEUDO-RESPONSE REGULATOR9 form a positive feedback regulatory loop in the Arabidopsis circadian clock. Plant Cell 23: 486–498.

  • Chen HM, Chen LT, Patel K, Li YH., Baulcombe D, and Wu SH* (2010) 22-nucleotide RNA triggers secondary siRNA biogenesis in plants.  Proc. Natl. Acad. Sci. USA 107:15269-15274.

  • Khanna R*, Kronmiller BA, Maszle DR, Coupland G, Holm M, Mizuno T, and Wu SH. (2009) The Arabidopsis B-box zinc finger family. Plant Cell 21:3416-3420.

  • Wu JF, Wang Y, and Wu SH.* (2008) Two new clock proteins, LWD1 and LWD2, regulate Arabidopsis photoperiodic flowering. Plant Physiol. 148:948-959

  • Chang CS, Li YH, Chen LT, Chen WC, Hsieh WP, Shin J, Jane WN, Chou SJ, Choi G, Hu JM, Somerville S, and Wu SH.* (2008) LZF1, a HY5-regulated transcriptional factor, functions in Arabidopsis de-etiolation. Plant J. 54:205-219.

  • Chen HM, Li YH, and Wu SH.* (2007) Bioinformatic prediction and experimental validation of a microRNA-directed tandem trans-acting siRNA cascade in Arabidopsis. Proc. Natl. Acad. Sci. USA 104:3318-3323.

 

     
  Jing-Fen Wu
Research assistant
fenny@gate.sinica.edu.tw
     
  Yi-Chen Wu
Research assistant
wyc9823@gate.sinica.edu.tw
     
  Guan-Hong Chen
Postdoc
ken1214@gate.sinica.edu.tw
     
  Chih-Ching Lin
Postdoc
f92b42009@ntu.edu.tw
     
 

Sim Lin Lim
Postdoc
simlinlim86@gate.sinica.edu.tw

     
 

Chun-Kai Huang
Postdoc
hck0903@gate.sinica.edu.tw

     
  徐思維 Ssu-Wei Hsu 
Postdoc
swh@gate.sinica.edu.tw
     
  Wen-Yu Huang
PhD student
wenyuhuang523@gmail.com
     
  Meng-Chun Lin
PhD student
f97b42012@ntu.edu.tw
     
  Geng-Jen Jang
PhD student
gang@gate.sinica.edu.tw
     
  Ho-Wei Wu
PhD student
b99b01053@ntu.edu.tw
     
  林岑穎 Tsen-Ying Lin 
MS student
crosswing@gmail.com