謝明勳

謝明勳
Hsieh, Ming-Hsiun
副研究員

Ph.D., Department of Biology, New York University, USA
M.S., Department of Botany, National Taiwan University
B.S., Department of Botany, National Taiwan University

TEL: 

+886-2-2787-1168 (Office)
+886-2-2787-1046 (Lab)

一、粒線體與葉綠體基因表現的調控

植物粒線體與葉綠體的基因在轉錄之後,必須經過複雜的RNA cleavage、editing與splicing之後,才能轉譯成具有功能的蛋白質,本實驗室即著重於研究PPR蛋白族群在這些後轉錄層次的調控。我們以阿拉伯芥為研究材料,篩選到了一些生長緩慢(slow growthslo)與白色或淡綠色的突變株。先前的研究顯示, SLO1 PPR是粒線體內nad4-449和nad9-328的editing factor,而最近的研究發現SLO3 PPR則是粒線體內nad7 intron 2的 splicing factor(圖一),我們將進一步研究SLO3如何在粒線體內參與nad7 intron 2的剪接。另外,我們從阿拉伯芥淡綠色突變株的研究中,找到了一個與粒線體或葉綠體內tRNA修飾有關的蛋白質,此蛋白質應該在粒線體或葉綠體內的轉譯作用中,扮演著重要的角色。

二、基酸的感應與訊息傳導

我們在阿拉伯芥發現了一群具有二至四個 ACT (aspartate kinase, chorismate mutase and TyrA) domains 的蛋白質,並將這群蛋白質命名為 ACR (ACT repeat) 蛋白。很多與胺基酸合成有關的酵素,都有 ACT domain 與 catalytic domain。當胺基酸過剩時,便會透過 ACT domain 來迴饋抑制 catalytic domain 的活性。然而 ACR 蛋白質除了 ACT domain 之外,並沒有任何的 catalytic domain。因此,我們推測 ACR 蛋白質可能具有感應胺基酸的功能。我們正以分子遺傳的研究方法,對這群蛋白質在阿拉伯芥裡 loss-of-function 與 gain-of-function 的植株進行研究,以釐清這群蛋白質的生理功能。另外,我們也以 forward genetics 的研究方法,篩選出一些在以胺基酸作為唯一氮源的培養基中,生長或發育會受到抑制的阿拉伯芥突變株(圖二),這些突變株可能對胺基酸無法正常地的吸收、代謝、運送或感應,我們將進一步研究這些突變株與胺基酸的感應與訊息傳導有什麼關係。

 


  Fig. 1 (A) 6-week-old Arabidopsis wild type (WT), slo3 and slo3 complemented by 35S:SLO3-GFP grown in soil.
            (B) Subcellular localization of SLO3-GFP in the root hair of a 35S:SLO3-GFP transgenic plant. The SLO3-GFP is localized to the mitochondrion.

 


Fig. 2
The growth of an Arabidopsis mutant (216-2A) was inhibited in the presence of amino acid.

 

All publication list

 

Selected publication list

 

  • Yang HC, Kan CC, Hung TH, Hsieh PH, Wang SY, Hsieh WY, Hsieh MH* (2017) Identification of early ammonium nitrate-responsive genes in rice roots. Sci Rep 7: 16885
     
  • Hsieh WY, Liao JC, Wang HT, HungTH, Tseng CC, Chung TY, MH Hsieh* (2017) The Arabidopsis thiamin deficient mutant pale green1 lacks thiamin monophosphate phosphatase of the vitamin B1 biosynthesis pathway. Plant J 91: 145-157
     
  • KanCC, ChungTY, Wu HY, Juo YA, Hsieh MH* (2017) Exogenous glutamate rapidly induces the expression of genes involved in metabolism and defense responses in rice roots. BMC Genomics 18:186
     
  • Liao JC, Hsieh WY, Tseng CC, Hsieh MH* (2016) Dysfunctional chloroplasts up-regulate the expression of mitochondrial genes in Arabidopsis seedlings. Photosynth Res127:151–159
     
  • Hsieh WY, Liao, JC, Hsieh MH* (2015) Dysfunctional mitochondria regulate the size of root apical meristem and leaf development in Arabidopsis. Plant Signal Behav 10: 10, e1071002-4
     
  • Kan CC, Chung TY, Juo YA, Hsieh MH* (2015) Glutamine rapidly induces the expression of key transcription factor genes involved in nitrogen and stress responses in rice roots. BMC Genomics 16: 731
     
  • Kan CC, Chung TY, Hsieh MH* (2015) Gene expression profiling of rice seedlings in response to glutamine treatment. Genomics Data 6:123-124
     
  • Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH* (2015) The SLOW GROWTH3 pentatricopeptide repeat protein is required for the splicing of mitochondrial NADH dehydrogenase subunit7 intron 2 in Arabidopsis. Plant Physiol 168: 490-501
     
  • Hsieh WY, Hsieh MH* (2015) The amino-terminal conserved domain of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase is critical for its function in oxygen-evolving photosynthetic organisms. Plant Signal Behav 10:2, e988072
     
  • Hsieh WY, Sung TY, Wang HT, Hsieh MH* (2014) Functional evidence for the critical amino-terminal conserved domain and key amino acids of Arabidopsis 4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE. Plant Physiol 166: 57-69

實驗室成員:

 

Lab Members

夏許 (博士後)

Shashi Kant Singh (Postdoc)

夏卡蜜 (博士後)

Camille Chardin (Postdoc)

王幸美 (博士後)

Hsin-Mei Wang (Postdoc)

桑竹 (研究助理)

San-Chu Lin (Research Assistant)

謝秉翰(研究助理)

Ping-Han Hsieh (Research Assistant)

陳映竹(研究助理)

Ying-Jhu Chen (Research Assistant)

王詩云(研究助理)

Shih-Yun Wang (Research Assistant)

徐崇研究助理)

Chung-En Hsu (Research Assistant)

廖紘陞(大學生)

Hong-Sheng Liao (college student)