謝明勳

謝明勳
Hsieh, Ming-Hsiun
副研究員

Ph.D., Department of Biology, New York University, USA
M.S., Department of Botany, National Taiwan University
B.S., Department of Botany, National Taiwan University

TEL: 

+886-2-2787-1168 (Office)
+886-2-2787-1046 (Lab)

Website: 

植物分子營養與訊息傳導

氮是植物最需要的養分之一,植物主要的氮源是土壤裡的硝酸或阿莫尼亞,這些無機氮必須在植物體內經過氮同化作用,以及一系列的轉氨作用,合成胺基酸以及其它含氮的有機化合物,才能供應植物生長與發育之所需。Glutamine是植物體內由無機氮轉化為有機氮的第一個產物,除了胺基酸、蛋白質與核酸的合成,glutamine在植物營養與訊息傳導可能也扮演著重要的角色。我們初步研究發現水稻可以很有效地利用glutamine做為氮源,促進植株的生長與發育(圖一)。此外,利用基因體學的研究方法,我們在水稻找到了一些對glutamine、glutamate及ammonium nitrate反應特別敏感的基因(圖一),這些基因可能與氮的代謝、感應與訊息傳導有關,我們正在研究這些基因的功能。

 

圖一、水稻可以有效地吸收利用Gln(左),水稻根部受到Gln, Glu及 NH4NO3 快速誘導基因之分析 (右)。

 

除了水稻,我們也以阿拉伯芥做為研究材料,利用forward genetics 的研究方法,篩選出一些在以glutamine作為唯一氮源時,生長或發育會受到抑制的阿拉伯芥突變株(圖二),這些突變株可能對glutamine無法正常地的吸收、運送、代謝或感應,我們將進一步研究這些突變株與glutamine的感應與訊息傳導有什麼關係。另外,我們先前的研究在阿拉伯芥發現了一群具有二至四個 ACT domain 的蛋白質,並將這群蛋白質命名為 ACR (ACT repeat)。很多與胺基酸合成有關的酵素,都有 ACT domain 與 catalytic domain。當胺基酸過剩時,便會透過 ACT domain 來迴饋抑制 catalytic domain 的活性。然而 ACR 蛋白質除了 ACT domain 之外,並沒有任何的 catalytic domain。因此,我們推測 ACR 蛋白質可能具有感應胺基酸的功能。阿拉伯芥共有十二個ACR基因(ACR1-ACR12),我們已獲得大部分ACR基因的突變株,例如圖三即為acr11的突變株,我們將利用這群突變株,來研究阿拉伯芥ACR基因的功能。

 

 

圖二、阿拉伯芥dig1 (defective in glutamine1) 的性狀分析。

 

圖三、阿拉伯芥acr11突變株的性狀分析。

 

All publication list

 

Selected publication list

 

  1. Yang HC, Kan CC, Hung TH, Hsieh PH, Wang SY, Hsieh WY, Hsieh MH* (2017) Identification of early ammonium nitrate-responsive genes in rice roots. Sci Rep 7: 16885
  2. Hsieh WY, Liao JC, Wang HT, HungTH, Tseng CC, Chung TY, MH Hsieh* (2017) The Arabidopsis thiamin deficient mutant pale green1 lacks thiamin monophosphate phosphatase of the vitamin B1 biosynthesis pathway. Plant J 91: 145-157
  3. KanCC, ChungTY, Wu HY, Juo YA, Hsieh MH* (2017) Exogenous glutamate rapidly induces the expression of genes involved in metabolism and defense responses in rice roots. BMC Genomics 18:186
  4. Leu KC, Hsieh MH, Hsieh HL, Wang HJ, Jauh GY (2016) Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing. RNA Biol 13: 593-604
  5. Liao JC, Hsieh WY, Tseng CC, Hsieh MH* (2016) Dysfunctional chloroplasts up-regulate the expression of mitochondrial genes in Arabidopsis seedlings. Photosynth Res127:151–159
  6. Hsieh WY, Liao, JC, Hsieh MH* (2015) Dysfunctional mitochondria regulate the size of root apical meristem and leaf development in Arabidopsis. Plant Signal Behav 10: 10, e1071002-4
  7. Kan CC, Chung TY, Juo YA, Hsieh MH* (2015) Glutamine rapidly induces the expression of key transcription factor genes involved in nitrogen and stress responses in rice roots. BMC Genomics 16: 731
  8. Kan CC, Chung TY, Hsieh MH* (2015) Gene expression profiling of rice seedlings in response to glutamine treatment. Genomics Data 6:123-124
  9. Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH* (2015) The SLOW GROWTH3 pentatricopeptide repeat protein is required for the splicing of mitochondrial NADH dehydrogenase subunit7 intron 2 in Arabidopsis. Plant Physiol 168: 490-501
  10. Hsieh WY, Hsieh MH* (2015) The amino-terminal conserved domain of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase is critical for its function in oxygen-evolving photosynthetic organisms. Plant Signal Behav 10:2, e988072
  11. Hsu YW, Wang HJ, Hsieh MH, Hsieh HL, Jauh GY (2014) Arabidopsis mTERF15 is required for mitochondrial nad2 intron 3 splicing and functional complex I activity. PLoS One 9: e112360
  12. Hsieh WY, Sung TY, Wang HT, Hsieh MH* (2014) Functional evidence for the critical amino-terminal conserved domain and key amino acids of Arabidopsis 4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE. Plant Physiol 166: 57-69
  13. Tseng CC, Lee CJ, Chung YT, Sung TY, Hsieh MH* (2013) Differential regulation of Arabidopsis plastid gene expression and RNA editing in non-photosynthetic tissues. Plant Mol Biol 82: 375-392
  14. Sung TY, Chung TY, Hsu CP, Hsieh MH* (2011) The ACR11 encodes a novel type of chloroplastic ACT domain repeat protein that is coordinately expressed with GLN2 in Arabidopsis. BMC Plant Biol 11:118

實驗室成員:

 

Lab Members

夏許 (博士後)

Shashi Kant Singh (Postdoc)

夏卡蜜 (博士後)

Camille Chardin (Postdoc)

王幸美 (博士後)

Hsin-Mei Wang (Postdoc)

桑竹 (研究助理)

San-Chu Lin (Research Assistant)

謝秉翰(研究助理)

Ping-Han Hsieh (Research Assistant)

陳映竹(研究助理)

Ying-Jhu Chen (Research Assistant)

王詩云(研究助理)

Shih-Yun Wang (Research Assistant)

徐崇研究助理)

Chung-En Hsu (Research Assistant)

廖紘陞(大學生)

Hong-Sheng Liao (college student)