賴爾珉

賴爾珉
Lai, Erh-Min
研究員

副所長

美國加州大學戴維斯分校博士

TEL: 

+886-2-2787-1158 (Office)
+886-2-2787-1031 (Lab)

本實驗室研究方向主要為探討植物病原細菌-農桿菌與宿主間相互作用之分子機制。農桿菌為植物腫瘤病之病原菌,可感染許多雙子葉植物。農桿菌會利用第四型蛋白質分泌系統(T4SS)將T-DNA 轉移至植物細胞中進而使植物細胞轉型為腫瘤。這種”跨界”的DNA移轉使農桿菌成為研究植物分子生物學最炙手可熱的轉基因工具。雖然多年來對於農桿菌致病基因及其分子功能的研究已有相當程度的瞭解; 然而, 對於農桿菌如何利用T4SS運送T-DNA穿過植物細胞壁及細胞膜以及克服宿主植物防禦系統進而成功感染的相關機制仍然不清楚。

蛋白質分泌系統在病原性細菌的致病能力及生存上扮演重要角色。我們目前主要研究農桿菌的兩個主要蛋白質分泌系統(T4SS及T6SS)其生理及生化功能。我們研究 T線毛在T4SS DNA轉移的角色及T6SS如何調節,組裝,及其分泌蛋白質在農桿菌與宿主植物交互作用中所扮演的角色。目前的研究成果發現農桿菌T6SS主要由14個蛋白質所組成,農桿菌其T6SS會在酸性環境中透過ExoR-ChvG/ChvI訊息傳導路徑及磷酸化TssL而誘導T6SS的表現,組裝及分泌。近期研究發現農桿菌在特定時機會分泌一種名為Tde ( type VI DNase effectors) 的DNA分解酶蛋白質,利用第六型分泌系統將Tde注射到所接觸到的其他細菌競爭對手的細胞內,以分解競爭對手的DNA,達到確保自己在植物宿主內的生存優勢,展現細菌生存攻防戰的新策略,此研究成果未來或可應用於醫藥物開發及農業改良 。

藉由研究農桿菌的蛋白質分泌系統其生理及生化功能,我們期望所獲得之研究成果及新知除可用於提高植物基因轉殖效率外,更可運用於相關藥物開發進而控制動植物細菌病原菌之感染。

圖一、農桿菌為一種植物病原細菌,可藉由第四型蛋白質分泌系統(type IV secretion system, T4SS) 將 T-DNA 傳送進入植物基因體中而造成癌腫病(美國加州大學戴維斯分校的榆樹癌腫病, 胡哲明先生拍攝)。藉由農桿菌傳送DNA來進行植物的短暫基因表達能夠提供一種簡單又快速的方法來分析基因的功能。因此我們發展出一種利用農桿菌於阿拉伯芥小苗進行高效率的基因短暫表達系統,稱為AGROBEST,可應用在基因功能性的分析 (Wu et al., 2014. Plant Methods)。

 

圖二、組成農桿菌T6SS及分泌蛋白質之基因主要位於農桿菌之線性染色體上約20-kb片斷之基因群。在酸性環境中,農桿菌透過ExoR-ChvG/ChvI訊息傳導路徑及磷酸化TssL而誘導T6SS的表現,組裝及分泌 (Wu, Lin et al., PLoS Pathogens, 2012; Lin et al., PLoS Pathogens, 2014)。

 

圖三、農桿菌利用第六型分泌系統DNA分解酶以增進其在植物寄主中的生存優勢 (Ma et al., Cell Host Microbe, 2014)。

All publication list

 

Selected publication list

 

  • Bondage D, Lin JS, Ma LS, Kuo CH, and Lai EM* (2016) VgrG C-terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex. Proc Natl Acad Sci U S A 2016 Jul 5;113(27):E3931-40. doi: 10.1073/pnas.1600428113.

  • Hwang HH, Gelvin SB, Lai EM*. (2015) Editorial: Agrobacterium biology and its application to transgenic plant production.Front Plant Sci. 6:265. doi: 10.3389/fpls.2015.00265.

  • Wu HY, Chen CY, and Lai EM.* (2014) Molecular functions of VirB2 in T-pilus assembly and virulence of Agrobacterium tumefaciens. PLoS One 9(6):e101142. doi: 10.1371/journal.pone.0101142.

  • Ma LS, Hachani A, Lin JS, Filloux A*, and Lai EM.* (2014) A superfamily of type VI secretion DNases provides Agrobacterium tumefaciens with a competitive advantage in planta. Cell Host & Microbe. 16(1):94-104. doi: 10.1016/j.chom.2014.06.002.

  • Wu HY, Liu KH, Wang YC, Wu JF, Chiu WL, Chen CY, Wu SH, Sheen J, and Lai EM.* (2014) AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analysis in Arabidopsis seedlings. Plant Methods. 10: 19. doi: 10.1186/1746-4811-10-19.

  • Lin, J. S., Wu, H. H., Hsu, P. H., Ma, L. S., Pang, Y. Y., Tsai, M. D., and Lai, E. M.* (2014) Fha interaction with phosphothreonine of TssL activates type VI secretion in Agrobacterium tumefaciens. PLoS Pathog.10(3):e1003991.

  • Lin, J. S., Ma, L. S., and Lai, E. M.* (2013) Systematic dissection of the Agrobacterium type VI secretion system reveals machinery and secreted components for subcomplex formation. PLoS One 8(7):e67647. doi: 10.1371/journal.pone.0067647.

  • Tsai, Y. L, Chiang, Y. R, Wu, C. F, Narberhaus, F, and Lai, E. M.*(2012) One out of four: HspL but no other small heat shock protein of Agrobacterium tumefaciens acts as efficient virulence-promoting VirB8 chaperone. PLoS One 7(11): e49685. (The first two authors contributed equally to this work).

  • Wu, C. F., Lin, J. S., G. C. Shaw, * and Lai, E. M.* (2012) Acid-induced type VI secretion system is regulated by ExoR-ChvG/ChvI signaling cascade in Agrobacterium tumefaciens.PLoS Pathog. 8(9): e1002938. (The first two authors contributed equally to this work)

  • Ma, L. S., Narberhaus F., and Lai, E. M.* (2012) The IcmF-family protein TssM exhibits ATPase activity and energizes type VI secretion. J. Biol. Chem. 287 (19):15610-15621.

  • Tsai, Y. L., Chiang, Y. R., Baron, C., Narberhaus, F., and Lai, E. M.* (2010) Small heat-shock protein HspL is a VirB8 chaperone promoting Type IV secretion-mediated DNA transfer. J. Biol. Chem. 285 (26):19757-19766.

  • Hwang, H. H.,* Wang, M. H., Lee, Y. L., Li, Y. H., Yang, F. J., Liao, Y. C., Tsai, Y. L., Lin, S. K., and Lai, E. M.*(2010) Agrobacteirum-produced and exogenous  cytokinins modulate Agrobacterium­-mediated plant transformation. Mol. Plant Path. 11(5), 677–690.

  • Ma, L., Lin, J. S., and Lai, E. M.* (2009) An IcmF-family protein ImpLM is an integral inner membrane protein interacting with ImpKL and its Walker A motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J. Bacteriol. 191:4316-4329. (selected by Faculty 1000)

  • Wu, H. Y., Chung, P. C., Shih, H. W., Wen, S. R., and Lai, E. M.* (2008) Secretome analysis uncovers an Hcp-family protein secreted via a type VI secretion system in Agrobacterium tumefaciens. J. Bacteriol. 190:2841-2850. (selected by Faculty 1000)

  • Lin, Y. Z., Chen, H. Y., Chang, S. P., Kao, R., Chang, S. J., and Lai, E. M.* (2008) Proteomic analysis of rice defense response induced by probenazole. Phytochemistry69: 715-728.

  • Lai, E. M.*, Shih, H. W., Wen, S. R., Cheng, M. W., Hwang, H. H., and Chiu, S. H. (2006) Proteomic analysis of Agrobacterium tumefaciens response to the vir gene inducer acetosyringone. Proteomics6: 4130-4136.

無標題文件

 

賴老師的另外一面
Life of Dr. Lai outside of the lab

余文迪 Manda Yu
博士後研究
Postdoctoral Research Fellow
mandayu@gate.sinica.edu.tw
 
帝凡內 Devanand D Bondage
博士後研究
Postdoctoral Research Fellow
dev@gate.sinica.edu.tw
 
施博原 Po-Yuan Shih
博士生 Ph.D. student
spuchris@gmail.com
 
妮亞 Nia Santos
博士生 Ph.D. student
maryniasantos@gmail.com
 

林筱涵 Hsiao-Han Lin
博士生 Ph.D. student
d01642010@ntu.edu.tw

 

連允薇 Yun-Wei Lien
碩士生 Master student
b02613001@ntu.edu.tw

 
王奕杰 Yi-Chieh Wang
研究助理 Research Assistant
apowerled@yahoo.com.tw