賴爾珉

賴爾珉
Lai, Erh-Min
研究員

副所長

美國加州大學戴維斯分校博士

TEL: 

+886-2-2787-1158 (Office)
+886-2-2787-1031 (Lab)

微生物無所不在目前科學研究證據充分顯示微生物可說是攸關地球上所有動植物生存或健康的必要元素因此研究微生物之間或與真核寄主的互動關係對人類和農作物的生長發育及其健康是非常重要及熱門的研究課題我實驗室的主要研究方向為探討病原細菌( 以農桿菌為模式生物) 如何運用它的蛋白質分泌機制來增進其在環境中與其他微生物競爭或在寄主中的生存優勢農桿菌為一種普遍存在於土壤中的植物病原細菌可藉由第四型蛋白質分泌系統 (type IV secretion system, T4SS) T-DNA傳送進入植物基因體中而造成癌腫病由於其跨界的DNA轉移能力農桿菌有遺傳工程師之稱透過它的基因轉殖能力人類得以將外來基因導入植物細胞內研發出各種基因轉殖作物 本實驗室主要為研究第四型及第六型蛋白質分泌系統 (type IV and typeVI secretion systems, T4SS andT6SS) 的生化及生理功能進而探討農桿菌如何運用蛋白質分泌系統來增進其感染力植物的基因轉殖效率及在環境中的生存競爭力 我們期望所獲得之研究成果及新知除可用於提高植物基因轉殖效率外更可運用於相關藥物開發進而控制動植物細菌病原菌之感染

圖一、農桿菌為一種植物病原細菌,可藉由第四型蛋白質分泌系統(type IV secretion system, T4SS) 將 T-DNA 傳送進入植物基因體中而造成癌腫病(美國加州大學戴維斯分校的榆樹癌腫病, 胡哲明先生拍攝)。藉由農桿菌傳送DNA來進行植物的短暫基因表達能夠提供一種簡單又快速的方法來分析基因的功能。因此我們發展出一種利用農桿菌於阿拉伯芥小苗進行高效率的基因短暫表達系統,稱為AGROBEST,可應用在基因功能性的分析 (Wu et al., 2014. Plant Methods)。

 

圖二、組成農桿菌T6SS及分泌蛋白質之基因主要位於農桿菌之線性染色體上約20-kb片斷之基因群。在酸性環境中,農桿菌透過ExoR-ChvG/ChvI訊息傳導路徑及磷酸化TssL而誘導T6SS的表現,組裝及分泌 (Wu, Lin et al., PLoS Pathogens, 2012; Lin et al., PLoS Pathogens, 2014)。

 

圖三、農桿菌利用第六型分泌系統DNA分解酶以增進其在植物寄主中的生存優勢 (Ma et al., Cell Host Microbe, 2014)。

All publication list

 

Selected publication list

 

  • Yu M and Lai EM* (2017) Warfare between Host Immunity and Bacterial Weapons. Cell Host & Microbe Jan 11;21(1):3-4.
    doi: 10.1016/j.chom.2016.12.012.

  • Bondage D, Lin JS, Ma LS, Kuo CH, and Lai EM* (2016) VgrG C-terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex. Proc Natl Acad Sci U S A 2016 Jul 5;113(27):E3931-40. doi: 10.1073/pnas.1600428113.

  • Hwang HH, Gelvin SB, Lai EM*. (2015) Editorial: Agrobacterium biology and its application to transgenic plant production.Front Plant Sci. 6:265. doi: 10.3389/fpls.2015.00265.

  • Wu HY, Chen CY, and Lai EM.* (2014) Molecular functions of VirB2 in T-pilus assembly and virulence of Agrobacterium tumefaciens. PLoS One 9(6):e101142. doi: 10.1371/journal.pone.0101142.

  • Ma LS, Hachani A, Lin JS, Filloux A*, and Lai EM.* (2014) A superfamily of type VI secretion DNases provides Agrobacterium tumefaciens with a competitive advantage in planta. Cell Host & Microbe. 16(1):94-104. doi: 10.1016/j.chom.2014.06.002.

  • Wu HY, Liu KH, Wang YC, Wu JF, Chiu WL, Chen CY, Wu SH, Sheen J, and Lai EM.* (2014) AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analysis in Arabidopsis seedlings. Plant Methods. 10: 19. doi: 10.1186/1746-4811-10-19.

  • Lin, J. S., Wu, H. H., Hsu, P. H., Ma, L. S., Pang, Y. Y., Tsai, M. D., and Lai, E. M.* (2014) Fha interaction with phosphothreonine of TssL activates type VI secretion in Agrobacterium tumefaciens. PLoS Pathog.10(3):e1003991.

  • Lin, J. S., Ma, L. S., and Lai, E. M.* (2013) Systematic dissection of the Agrobacterium type VI secretion system reveals machinery and secreted components for subcomplex formation. PLoS One 8(7):e67647. doi: 10.1371/journal.pone.0067647.

  • Tsai, Y. L, Chiang, Y. R, Wu, C. F, Narberhaus, F, and Lai, E. M.*(2012) One out of four: HspL but no other small heat shock protein of Agrobacterium tumefaciens acts as efficient virulence-promoting VirB8 chaperone. PLoS One 7(11): e49685. (The first two authors contributed equally to this work).

  • Wu, C. F., Lin, J. S., G. C. Shaw, * and Lai, E. M.* (2012) Acid-induced type VI secretion system is regulated by ExoR-ChvG/ChvI signaling cascade in Agrobacterium tumefaciens.PLoS Pathog. 8(9): e1002938. (The first two authors contributed equally to this work)

  • Ma, L. S., Narberhaus F., and Lai, E. M.* (2012) The IcmF-family protein TssM exhibits ATPase activity and energizes type VI secretion. J. Biol. Chem. 287 (19):15610-15621.

  • Tsai, Y. L., Chiang, Y. R., Baron, C., Narberhaus, F., and Lai, E. M.* (2010) Small heat-shock protein HspL is a VirB8 chaperone promoting Type IV secretion-mediated DNA transfer. J. Biol. Chem. 285 (26):19757-19766.

  • Hwang, H. H.,* Wang, M. H., Lee, Y. L., Li, Y. H., Yang, F. J., Liao, Y. C., Tsai, Y. L., Lin, S. K., and Lai, E. M.*(2010) Agrobacteirum-produced and exogenous  cytokinins modulate Agrobacterium­-mediated plant transformation. Mol. Plant Path. 11(5), 677–690.

  • Ma, L., Lin, J. S., and Lai, E. M.* (2009) An IcmF-family protein ImpLM is an integral inner membrane protein interacting with ImpKL and its Walker A motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J. Bacteriol. 191:4316-4329. (selected by Faculty 1000)

  • Wu, H. Y., Chung, P. C., Shih, H. W., Wen, S. R., and Lai, E. M.* (2008) Secretome analysis uncovers an Hcp-family protein secreted via a type VI secretion system in Agrobacterium tumefaciens. J. Bacteriol. 190:2841-2850. (selected by Faculty 1000)

  • Lin, Y. Z., Chen, H. Y., Chang, S. P., Kao, R., Chang, S. J., and Lai, E. M.* (2008) Proteomic analysis of rice defense response induced by probenazole. Phytochemistry69: 715-728.

  • Lai, E. M.*, Shih, H. W., Wen, S. R., Cheng, M. W., Hwang, H. H., and Chiu, S. H. (2006) Proteomic analysis of Agrobacterium tumefaciens response to the vir gene inducer acetosyringone. Proteomics6: 4130-4136.

 

   

楊慶鴻 Ching-Hong Yang
chyang@uwm.edu
訪問教授 Visiting Professor
Professor, Department of 
Biological Sciences, University
of Wisconsin-Milwaukee, USA

   
余文迪 Manda Yu
博士後研究
Postdoctoral Research Fellow
mandayu@gate.sinica.edu.tw
 

簡靖芳 Ching-Fang Chien
博士後研究
Postdoctoral Research Fellow
d99623004@ntu.edu.tw

 
施博原 Po-Yuan Shih
博士生 Ph.D. student
spuchris@gmail.com
 
妮亞 Nia Santos
博士生 Ph.D. student
maryniasantos@gmail.com
 

林筱涵 Hsiao-Han Lin
博士生 Ph.D. student
d01642010@ntu.edu.tw

 

狄杰莫 Jemal Ali
博士生 Ph.D. student

jamesbiot@yahoo.com

   

連允薇 Yun-Wei Lien
碩士生 Master student
b02613001@ntu.edu.tw

 
王奕杰 Yi-Chieh Wang
研究助理 Research Assistant
apowerled@yahoo.com.tw
   

張君如 Chun-Ju Chang
研究助理 Research Assistant
chunju77527@gmail.com

  
         

李佳 Chia Lee
研究助理 Research Assistant
b01406021@ntu.edu.tw